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AB70110

Anti-CK1 epsilon antibody

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(2 Publications)

Rabbit Polyclonal CK1 epsilon antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Casein kinase I isoform epsilon.

View Alternative Names

Casein kinase I isoform epsilon, CKI-epsilon, CKIe, CSNK1E

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CK1 epsilon antibody (AB70110)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CK1 epsilon antibody (AB70110)

ab70110 at 1/50 dilution staining CK1 epsilon in human placenta by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide

Immunocytochemistry/ Immunofluorescence - Anti-CK1 epsilon antibody (AB70110)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CK1 epsilon antibody (AB70110)

ab70110 at 1/500 dilution staining CK1 epsilon in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Western blot - Anti-CK1 epsilon antibody (AB70110)
  • WB

Unknown

Western blot - Anti-CK1 epsilon antibody (AB70110)

All lanes:

Western blot - Anti-CK1 epsilon antibody (ab70110) at 1/500 dilution

Lane 1:

LOVO cell extracts at 30 µg

Lane 2:

LOVO cell extracts at 30 µg with immunising peptide

Predicted band size: 47 kDa

Observed band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Casein kinase I isoform epsilon. The exact immunogen used to generate this antibody is proprietary information.

P49674

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab70110 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates (Probable). Participates in Wnt signaling (PubMed : 12556519, PubMed : 23413191). Phosphorylates DVL1 (PubMed : 12556519). Phosphorylates DVL2 (PubMed : 23413191). Phosphorylates NEDD9/HEF1 (By similarity). Central component of the circadian clock (PubMed : 16790549). In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation (PubMed : 15917222, PubMed : 16790549). Controls PER1 and PER2 nuclear transport and degradation (By similarity). Inhibits cytokine-induced granuloytic differentiation (PubMed : 15070676).
See full target information Casein kinase I isoform epsilon
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 6:30343-56 PubMed26327509

2015

High casein kinase 1 epsilon levels are correlated with better prognosis in subsets of patients with breast cancer.

Applications

IHC

Species

Unspecified reactive species

Jose Luis Lopez-Guerra,Eva M Verdugo-Sivianes,Daniel Otero-Albiol,Begoña Vieites,Maria J Ortiz-Gordillo,Jose M De León,Juan M Praena-Fernandez,Juan J Marin,Amancio Carnero

PloS one 6:e25382 PubMed21966515

2011

The de-ubiquitinylating enzyme, USP2, is associated with the circadian clockwork and regulates its sensitivity to light.

Applications

WB

Species

Human

Heather Dehlin Scoma,Monica Humby,Geetha Yadav,Qingjiong Zhang,Joseph Fogerty,Joseph C Besharse
View all publications
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