Anti-CK1 epsilon antibody [EPR24332-25] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-CK1 epsilon antibody [EPR24332-25] - BSA and Azide free (AB282819)

This data was developed using ab270997, the same antibody clone in a different buffer formulation.

CK1 epsilon was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug with ab270997 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270997 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug

Lane 2 : ab270997 IP in 293T whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab270997 in 293T whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 15 seconds

All lanes:

Immunoprecipitation - Anti-CK1 epsilon antibody [EPR24332-25] (<a href='/en-us/products/primary-antibodies/ck1-epsilon-antibody-epr24332-25-ab270997'>ab270997</a>)

Predicted band size: 47 kDa

Observed band size: 40 kDa,43 kDa

false

• WB

Lab

Western blot - Anti-CK1 epsilon antibody [EPR24332-25] - BSA and Azide free (AB282819)

This data was developed using ab270997, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID : 30206363, 29044200).

Low expression tissue : human kidney (PMID : 15070676).

Exposure time : 103 seconds.

All lanes:

Western blot - Anti-CK1 epsilon antibody [EPR24332-25] (<a href='/en-us/products/primary-antibodies/ck1-epsilon-antibody-epr24332-25-ab270997'>ab270997</a>) at 1/1000 dilution

Lane 1:

Human lung tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 47 kDa

Observed band size: 40 kDa,43 kDa

false

• WB

Lab

Western blot - Anti-CK1 epsilon antibody [EPR24332-25] - BSA and Azide free (AB282819)

This data was developed using ab270997, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID : 30206363, 29044200).

Exposure time : 26 seconds

All lanes:

Western blot - Anti-CK1 epsilon antibody [EPR24332-25] (<a href='/en-us/products/primary-antibodies/ck1-epsilon-antibody-epr24332-25-ab270997'>ab270997</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 4:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 40 kDa,43 kDa

false