AB152154

Anti-CKAP4 antibody

Name: Anti-CKAP4 antibody (ab152154) | Abcam
Brand: Abcam Limited
SKU: AB152154
Price: 235 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal CKAP4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CKAP4 aa 200-600.

View Alternative Names

Cytoskeleton-associated protein 4, 63-kDa cytoskeleton-linking membrane protein, Climp-63, p63, CKAP4

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKAP4 antibody (AB152154)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKAP4 antibody (AB152154)

Immunohistochemical analysis of paraffin embedded CL1-5 xenograft tissue, labeling CKAP4 using ab152154 at 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKAP4 antibody (AB152154)

Immunohistochemical analysis of human colon cancer tissue stained for CKAP4 protein at cytoplasm and membrane with ab152154 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKAP4 antibody (AB152154)

Immunohistochemical analysis of mouse brain tissue stained for CKAP4 protein at cell membrane and cytoplasm with ab152154 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-CKAP4 antibody (AB152154)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-CKAP4 antibody (ab152154) at 1/10000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 66 kDa

false

Supplier Data

Western blot - Anti-CKAP4 antibody (AB152154)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-CKAP4 antibody (ab152154) at 1/1000 dilution

Lane 1:

Mouse stomach tissue extracts at 50 µg

Lane 2:

Rat stomach tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 66 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CKAP4 aa 200-600. The exact immunogen used to generate this antibody is proprietary information.

Q07065

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates the anchoring of the endoplasmic reticulum to microtubules.. High-affinity epithelial cell surface receptor for the FZD8-related low molecular weight sialoglycopeptide APF/antiproliferative factor. Mediates the APF antiproliferative signaling within cells.

See full target information CKAP4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 13:452-470 PubMed31911279

2020

Hallmarks and Determinants of Oncogenic Translation Revealed by Ribosome Profiling in Models of Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Christos Vaklavas,Scott W Blume,William E Grizzle

Oncology reports 39:2482-2498 PubMed29620220

2018

Translational control of the undifferentiated phenotype in ER‑positive breast tumor cells: Cytoplasmic localization of ERα and impact of IRES inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Christos Vaklavas,Kurt R Zinn,Sharon L Samuel,Zheng Meng,William E Grizzle,Hyoungsoo Choi,Scott W Blume

Molecular biology reports 42:1365-76 PubMed26047583

2015

Down-regulation of hsa-miR-1264 contributes to DNMT1-mediated silencing of SOCS3.

Applications

Unspecified application

Species

Unspecified reactive species

Chandra S Boosani,Kajari Dhar,Devendra K Agrawal

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CKAP4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKAP4 antibody (AB152154)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKAP4 antibody (AB152154)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKAP4 antibody (AB152154)

Western blot - Anti-CKAP4 antibody (AB152154)

Western blot - Anti-CKAP4 antibody (AB152154)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise