Rabbit Polyclonal CKAP4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CKAP4 aa 200-600.
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Publications
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- Translational oncology 13:452-4702020Hallmarks and Determinants of Oncogenic Translation Revealed by Ribosome Profiling in Models of Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristos Vaklavas et. al.PubMed 31911279
- Oncology reports 39:2482-24982018Translational control of the undifferentiated phenotype in ER‑positive breast tumor cells: Cytoplasmic localization of ERα and impact of IRES inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristos Vaklavas,Kurt R Zinn,Sharon L Samuel,Zheng Meng,William E Grizzle,Hyoungsoo Choi,Scott W BlumePubMed 29620220
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CKAP4 aa 200-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q07065
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Mediates the anchoring of the endoplasmic reticulum to microtubules. High-affinity epithelial cell surface receptor for the FZD8-related low molecular weight sialoglycopeptide APF/antiproliferative factor. Mediates the APF antiproliferative signaling within cells.
Alternative names
Cytoskeleton-associated protein 4, 63-kDa cytoskeleton-linking membrane protein, Climp-63, p63, CKAP4
Recommended products
Rabbit Polyclonal CKAP4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CKAP4 aa 200-600.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CKAP4 aa 200-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q07065
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-CKAP4 antibody (ab152154)
7.5% SDS PAGE
All lanes: Western blot - Anti-CKAP4 antibody (ab152154) at 1/10000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 66 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CKAP4 antibody (ab152154)
Immunofluorescence analysis of methanol fixed HeLa cells, labeling CKAP4 using ab152154 at 1/200 dilution. Lower image costained with Hoerchst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKAP4 antibody (ab152154)
Immunohistochemical analysis of paraffin embedded CL1-5 xenograft tissue, labeling CKAP4 using ab152154 at 1/500 dilution.
Western blot - Anti-CKAP4 antibody (ab152154)
All lanes: Western blot - Anti-CKAP4 antibody (ab152154) at 1/2000 dilution
All lanes: MDA-MB-231 whole cell lysate at 30 µg with 5% non-fat milk in TBST
Predicted band size: 66 kDa
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Product protocols
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