Rabbit Recombinant Monoclonal CKLF antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (AB250213), expandable thumbnail](https://content.abcam.com/products/images/cklf-antibody-epr119852-bsa-and-azide-free-ab250213--western-blot-img123300.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (AB250213)")
![Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (AB250213), expandable thumbnail](https://content.abcam.com/products/images/cklf-antibody-epr119852-bsa-and-azide-free-ab250213--western-blot-img123296.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (AB250213)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (AB250213), expandable thumbnail](https://content.abcam.com/products/images/cklf-antibody-epr119852-bsa-and-azide-free-ab250213--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45817.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (AB250213)")
Publications
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- Frontiers in oncology 11:7155522021Single-Cell RNA Sequencing Reveals the Migration of Osteoclasts in Giant Cell Tumor of Bone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenyu Feng,Mingwei He,Xiaohong Jiang,Huijiang Liu,Tianyu Xie,Zhaojie Qin,Qian Huang,Shijie Liao,Chengsen Lin,Juliang He,Jiake Xu,Jie Ma,Yun Liu,Qingjun WeiPubMed 34504794
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
May play an important role in inflammation and regeneration of skeletal muscle. Partly inhibited by interleukin 10.Isoform 1has chemotactic response in monocytes, neutrophils and lymphocytes (PubMed:11415443). Binds CCR4 (PubMed:16137713).
Alternative names
Chemokine-like factor, C32, CKLF1, HSPC224, UNQ410/PRO772, CKLF
Recommended products
Rabbit Recombinant Monoclonal CKLF antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.
Alternative names
Chemokine-like factor, C32, CKLF1, HSPC224, UNQ410/PRO772, CKLF
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR11985(2)
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab250213 is the carrier-free version of Anti-CKLF antibody [EPR11985(2)] - C-terminal ab180512.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213), expandable thumbnail](https://content.abcam.com/products/images/cklf-antibody-epr119852-bsa-and-azide-free-ab250213--western-blot-img123300.jpg "Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)")
Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)
This data was developed using Anti-CKLF antibody [EPR11985(2)] - C-terminal ab180512, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (Anti-CKLF antibody [EPR11985(2)] - C-terminal ab180512) at 1/5000 dilution
Lane 1: K562 cell lysate at 20 µg
Lane 2: HeLa cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
![Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213), expandable thumbnail](https://content.abcam.com/products/images/cklf-antibody-epr119852-bsa-and-azide-free-ab250213--western-blot-img123296.jpg "Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)")
Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)
This data was developed using Anti-CKLF antibody [EPR11985(2)] - C-terminal ab180512, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (Anti-CKLF antibody [EPR11985(2)] - C-terminal ab180512) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 20 µg
Lane 2: THP-1 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213), expandable thumbnail](https://content.abcam.com/products/images/cklf-antibody-epr119852-bsa-and-azide-free-ab250213--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45817.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)
This data was developed using Anti-CKLF antibody [EPR11985(2)] - C-terminal ab180512, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CKLF with Anti-CKLF antibody [EPR11985(2)] - C-terminal ab180512 at 1/100 dilution. Anti-rabbit HRP secondary antibody was used and counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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