AB180512

Anti-CKLF antibody [EPR11985(2)] - C-terminal

RabMAb
Recombinant
What is this?

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(8 Publications)

Rabbit Recombinant Monoclonal CKLF antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications.

View Alternative Names

CKLF1, HSPC224, UNQ410/PRO772, CKLF, Chemokine-like factor, C32

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF antibody [EPR11985(2)] - C-terminal (AB180512)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF antibody [EPR11985(2)] - C-terminal (AB180512)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CKLF with ab180512 at 1/100 dilution. Anti-rabbit HRP secondary antibody was used and counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (AB180512)

All lanes:

Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512) at 1/5000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Supplier Data

Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (AB180512)

All lanes:

Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

THP-1 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11985(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play an important role in inflammation and regeneration of skeletal muscle (PubMed : 11415443). Essential for embryonic development (By similarity).. Isoform 1. Has chemotactic response in monocytes, neutrophils and lymphocytes (PubMed : 11415443). Binds CCR4 (PubMed : 16137713).

See full target information CKLF

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 32:78 PubMed40836335

2025

Smooth muscle-specific expression of hydroxyindole O-methyltransferase reduces arterial injury-induced intimal hyperplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Cheng Jiang,Chung-Huang Chen,Hua-Hui Ho,Pei-Yu Gung,Jing-Yiing Wu,Cheng-Chin Kuo,Kenneth K Wu,Shaw-Fang Yet

Science advances 10:eadh9547 PubMed38489372

2024

CKLF instigates a "cold" microenvironment to promote MYCN-mediated tumor aggressiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodan Qin,Andrew Lam,Xu Zhang,Satyaki Sengupta,J Bryan Iorgulescu,Hongru Ni,Sanjukta Das,Madison Rager,Zhenwei Zhou,Tao Zuo,Grace K Meara,Alexander E Floru,Chinyere Kemet,Divya Veerapaneni,Daniel Kashy,Liang Lin,Kenneth Lloyd,Lauren Kwok,Kaylee S Smith,Raghavendar T Nagaraju,Rob Meijers,Craig Ceol,Ching-Ti Liu,Sanda Alexandrescu,Catherine J Wu,Derin B Keskin,Rani E George,Hui Feng

Cancer medicine 12:15704-15719 PubMed37306188

2023

Construction of a prognostic model for triple-negative breast cancer based on immune-related genes, and associations between the tumor immune microenvironment and immunological therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhu,Lin-Feng Tao,Jin-Yan Liu,Yi-Xuan Wang,Hai Huang,Yan-Nan Jiang,Wei-Feng Qian

Experimental and therapeutic medicine 25:303 PubMed37229323

2023

CKLF1 interference alleviates IL‑1β‑induced inflammation, apoptosis and degradation of the extracellular matrix in chondrocytes via CCR5.

Applications

Unspecified application

Species

Unspecified reactive species

Haoran Wang,Zhongqing Wu,Kanna Xu

Acta pharmacologica Sinica 43:1217-1230 PubMed34385606

2021

Neuronal chemokine-like-factor 1 (CKLF1) up-regulation promotes M1 polarization of microglia in rat brain after stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhou,Ya-Ni Zhang,Fang-Fang Li,Zhao Zhang,Li-Yuan Cui,Hong-Yuan He,Xu Yan,Wen-Bin He,Hong-Shuo Sun,Zhong-Ping Feng,Shi-Feng Chu,Nai-Hong Chen

Pediatric surgery international 37:1031-1040 PubMed34031745

2021

Establishment and characterization of an ovarian yolk sac tumor patient-derived xenograft model.

Applications

Unspecified application

Species

Unspecified reactive species

Manling Luo,Yuanqiao He,Baogang Xie,Shiyun Li,Fuqiang Gan,Shouhua Zhang,Puying Luo

FEBS open bio 10:1880-1890 PubMed32741140

2020

Chemokine-like factor 1 (CKLF1) aggravates neointimal hyperplasia through activating the NF-κB /VCAM-1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinnong Liu,Chengjia Qu,Yongbao Zhang,Jie Fang,Lequn Teng,Rujiao Zhang,Xiangyu Zhang,Chenyang Shen

Clinical cancer research : an official journal of 25:4141-4154 PubMed30918019

2019

CKLF1 Enhances Inflammation-Mediated Carcinogenesis and Prevents Doxorubicin-Induced Apoptosis via IL6/STAT3 Signaling in HCC.

Applications

Unspecified application

Species

Unspecified reactive species

Youyi Liu,Lijuan Liu,Yan Zhou,Ping Zhou,Qiujin Yan,Xiaobei Chen,Shuang Ding,Fan Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CKLF antibody [EPR11985(2)] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF antibody [EPR11985(2)] - C-terminal (AB180512)

Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (AB180512)

Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (AB180512)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (8)

Product promise