Rabbit Recombinant Monoclonal CLACP antibody. Suitable for Flow Cyt (Intra), WB and reacts with Rat, Mouse samples.
Images
![Western blot - Anti-CLACP antibody [EPR24205-82] (AB307538), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-ab307538--western-blot-img405775.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CLACP antibody [EPR24205-82] (AB307538)")
![Western blot - Anti-CLACP antibody [EPR24205-82] (AB307538), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-ab307538--western-blot-img405475.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CLACP antibody [EPR24205-82] (AB307538)")
![Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] (AB307538), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-ab307538--flow-cytometry-intracellular-img405474.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] (AB307538)")
![Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] (AB307538), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-ab307538--flow-cytometry-intracellular-img405473.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] (AB307538)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-Fr | IHC-P | WB | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Alternative names
Collagen alpha-1(XXV) chain, CLAC-P, Col25a1
Rabbit Recombinant Monoclonal CLACP antibody. Suitable for Flow Cyt (Intra), WB and reacts with Rat, Mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR24205-82
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CLACP antibody [EPR24205-82] (ab307538), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-ab307538--western-blot-img405775.jpg "Western blot - Anti-CLACP antibody [EPR24205-82] (ab307538)")
Western blot - Anti-CLACP antibody [EPR24205-82] (ab307538)
Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: kidney, liver (PMID: 11927537)
Exposure time: Lanes 1-2: 8 seconds, Lanes 3-6: 37 seconds.All lanes: Western blot - Anti-CLACP antibody [EPR24205-82] (ab307538) at 1/1000 dilution
Lane 1: FAD4T Transgenic mouse AlzheimerЎЇs disease brain tissue lysate 20 μg
Lane 2: Mouse brain tissue lysate 20 μg
Lane 3: Mouse cerebellum tissue lysate 20 μg
Lane 4: Mouse kidney tissue lysate 20 μg
Lane 5: Mouse liver tissue lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 110 kDa
Exposure time: 180s
![Western blot - Anti-CLACP antibody [EPR24205-82] (ab307538), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-ab307538--western-blot-img405475.jpg "Western blot - Anti-CLACP antibody [EPR24205-82] (ab307538)")
Western blot - Anti-CLACP antibody [EPR24205-82] (ab307538)
Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: kidney, liver (PMID: 11927537)
Exposure time: 180 secondsAll lanes: Western blot - Anti-CLACP antibody [EPR24205-82] (ab307538) at 1/1000 dilution
Lane 1: Rat brain tissue lysate 20 μg
Lane 2: Rat cerebellum tissue lysate 20 μg
Lane 3: Rat kidney tissue lysate 20 μg
Lane 4: Rat liver tissue lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 110 kDa
Exposure time: 180s
![Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] (ab307538), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-ab307538--flow-cytometry-intracellular-img405474.jpg "Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] (ab307538)")
Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] (ab307538)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized Rat primary neuron cell cells lABelling CLACP with ab307538 at 1/50 dilution (1ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
![Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] (ab307538), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-ab307538--flow-cytometry-intracellular-img405473.jpg "Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] (ab307538)")
Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] (ab307538)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized Mouse primary neuron cell cells lABelling CLACP with ab307538 at 1/50 dilution (1ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com