Rabbit Recombinant Monoclonal CLACP antibody. Carrier free. Suitable for Flow Cyt (Intra), WB and reacts with Rat, Mouse samples.
Images
![Western blot - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-bsa-and-azide-free-ab307539--western-blot-img405775.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539)")
![Western blot - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-bsa-and-azide-free-ab307539--western-blot-img405475.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539)")
![Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-bsa-and-azide-free-ab307539--flow-cytometry-intracellular-img405474.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539)")
![Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-bsa-and-azide-free-ab307539--flow-cytometry-intracellular-img405473.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-Fr | IHC-P | WB | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Alternative names
Collagen alpha-1(XXV) chain, CLAC-P, Col25a1
Rabbit Recombinant Monoclonal CLACP antibody. Carrier free. Suitable for Flow Cyt (Intra), WB and reacts with Rat, Mouse samples.
Alternative names
Collagen alpha-1(XXV) chain, CLAC-P, Col25a1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR24205-82
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (ab307539), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-bsa-and-azide-free-ab307539--western-blot-img405775.jpg "Western blot - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (ab307539)")
Western blot - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (ab307539)
This data was developed using 307538, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Negative control: kidney, liver (PMID: 11927537)
Lanes 1-2: 8 seconds, Lanes 3-6: 37 seconds.
Exposure time:All lanes: Western blot - Anti-CLACP antibody [EPR24205-82] (Anti-CLACP antibody [EPR24205-82] ab307538) at 1/1000 dilution
Lane 1: FAD4T Transgenic mouse AlzheimerЎЇs disease brain tissue lysate 20 μg
Lane 2: Mouse brain tissue lysate 20 μg
Lane 3: Mouse cerebellum tissue lysate 20 μg
Lane 4: Mouse kidney tissue lysate 20 μg
Lane 5: Mouse liver tissue lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 110 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: kidney, liver (PMID: 11927537)
Exposure time: Lanes 1-2: 8 seconds, Lanes 3-6: 37 seconds.![Western blot - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (ab307539), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-bsa-and-azide-free-ab307539--western-blot-img405475.jpg "Western blot - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (ab307539)")
Western blot - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (ab307539)
This data was developed using 307538, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Negative control: kidney, liver (PMID: 11927537)
180 seconds
Exposure time:All lanes: Western blot - Anti-CLACP antibody [EPR24205-82] (Anti-CLACP antibody [EPR24205-82] ab307538) at 1/1000 dilution
Lane 1: Rat brain tissue lysate 20 μg
Lane 2: Rat cerebellum tissue lysate 20 μg
Lane 3: Rat kidney tissue lysate 20 μg
Lane 4: Rat liver tissue lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 110 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: kidney, liver (PMID: 11927537)
Exposure time: 180 seconds![Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (ab307539), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-bsa-and-azide-free-ab307539--flow-cytometry-intracellular-img405474.jpg "Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (ab307539)")
Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (ab307539)
This data was developed using Anti-CLACP antibody [EPR24205-82] ab307538, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized Rat primary neuron cell cells lABelling CLACP with Anti-CLACP antibody [EPR24205-82] ab307538 at 1/50 dilution (1ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.![Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (ab307539), expandable thumbnail](https://content.abcam.com/products/images/clacp-antibody-epr24205-82-bsa-and-azide-free-ab307539--flow-cytometry-intracellular-img405473.jpg "Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (ab307539)")
Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (ab307539)
This data was developed using Anti-CLACP antibody [EPR24205-82] ab307538, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized Mouse primary neuron cell cells lABelling CLACP with Anti-CLACP antibody [EPR24205-82] ab307538 at 1/50 dilution (1ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
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