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AB307539

Anti-CLACP antibody [EPR24205-82] - BSA and Azide free

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Rabbit Recombinant Monoclonal CLACP antibody. Carrier free. Suitable for Flow Cyt (Intra), WB and reacts with Rat, Mouse samples.

View Alternative Names

Collagen alpha-1(XXV) chain, CLAC-P, Col25a1

4 Images
Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539)

This data was developed using ab307538, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cell cells labelling CLACP with ab307538 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539)

This data was developed using ab307538, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cell cells labelling CLACP with ab307538 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539)
  • WB

Supplier Data

Western blot - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539)

This data was developed using 307538, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Negative control : kidney, liver (PMID : 11927537) 180 seconds Exposure time :

All lanes:

Western blot - Anti-CLACP antibody [EPR24205-82] (<a href='/en-us/products/primary-antibodies/clacp-antibody-epr24205-82-ab307538'>ab307538</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate 20 μg

Lane 2:

Rat cerebellum tissue lysate 20 μg

Lane 3:

Rat kidney tissue lysate 20 μg

Lane 4:

Rat liver tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa

false

Exposure time: 180s

Western blot - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539)
  • WB

Supplier Data

Western blot - Anti-CLACP antibody [EPR24205-82] - BSA and Azide free (AB307539)

This data was developed using 307538, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Negative control : kidney, liver (PMID : 11927537) Lanes 1-2 : 8 seconds, Lanes 3-6 : 37 seconds. Exposure time :

All lanes:

Western blot - Anti-CLACP antibody [EPR24205-82] (<a href='/en-us/products/primary-antibodies/clacp-antibody-epr24205-82-ab307538'>ab307538</a>) at 1/1000 dilution

Lane 1:

FAD4T Transgenic mouse AlzheimerЎЇs disease brain tissue lysate 20 μg

Lane 2:

Mouse brain tissue lysate 20 μg

Lane 3:

Mouse cerebellum tissue lysate 20 μg

Lane 4:

Mouse kidney tissue lysate 20 μg

Lane 5:

Mouse liver tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24205-82

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits fibrillization of amyloid-beta peptide during the elongation phase. Has also been shown to assemble amyloid fibrils into protease-resistant aggregates. Binds heparin (By similarity).
See full target information Col25a1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com