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Rabbit Polyclonal CLASP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CLIP-associating protein 1 aa 1-200.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLASP1 antibody (AB154087), expandable thumbnail
  • Western blot - Anti-CLASP1 antibody (AB154087), expandable thumbnail
  • Western blot - Anti-CLASP1 antibody (AB154087), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CLASP1 antibody (AB154087), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human CLIP-associating protein 1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z460

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Expected
Tested
Tested
Mouse
Tested
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

1/100.00000 - 1/1000.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.

Alternative names

Recommended products

Rabbit Polyclonal CLASP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CLIP-associating protein 1 aa 1-200.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human CLIP-associating protein 1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z460
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLASP1 antibody (ab154087), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLASP1 antibody (ab154087)

    Immunohistochemical analysis of paraffin-embedded 4T1 xenograft tissue labeling CLASP1 with ab154087 at 1/100 dilution.

  • Western blot - Anti-CLASP1 antibody (ab154087), expandable thumbnail

    Western blot - Anti-CLASP1 antibody (ab154087)

    5% SDS PAGE

    All lanes: Western blot - Anti-CLASP1 antibody (ab154087) at 1/1000 dilution

    All lanes: Hep G2 whole cell lysate at 30 µg

    Predicted band size: 169 kDa

  • Western blot - Anti-CLASP1 antibody (ab154087), expandable thumbnail

    Western blot - Anti-CLASP1 antibody (ab154087)

    5% SDS PAGE

    All lanes: Western blot - Anti-CLASP1 antibody (ab154087) at 1/1000 dilution

    All lanes: Mouse brain whole cell lysate at 50 µg

    Predicted band size: 153 kDa, 169 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CLASP1 antibody (ab154087), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CLASP1 antibody (ab154087)

    Immunofluorescence analysis of methanol-fixed HeLa cells labeling CLASP1 with ab154087 at 1/500 dilution. The image in the lower panel is costained with Hoechst 33342.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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