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AB95373

Anti-CLASP2 antibody [KT68]

4

(2 Reviews)

|

(2 Publications)

Rat Monoclonal CLASP2 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CLASP2 aa 50-900.

View Alternative Names

KIAA0627, CLASP2, CLIP-associating protein 2, Cytoplasmic linker-associated protein 2, Protein Orbit homolog 2, hOrbit2

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLASP2 antibody [KT68] (AB95373)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLASP2 antibody [KT68] (AB95373)

IHC image of CLASP2 staining in Mouse Brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab95373, 1 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

KT68

Isotype

IgG2b

Carrier free

No

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CLASP2 aa 50-900. The exact immunogen used to generate this antibody is proprietary information.

O75122

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules (PubMed : 26003921). Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2 (PubMed : 16824950). This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (PubMed : 16866869, PubMed : 16914514). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.
See full target information CLASP2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Gastroenterology 157:1544-1555.e3 PubMed31473225

2019

Sperm Flagellar 1 Binds Actin in Intestinal Epithelial Cells and Contributes to Formation of Filopodia and Lamellipodia.

Applications

Unspecified application

Species

Unspecified reactive species

Rocio Tapia,Eloy A Perez-Yepez,Maximillian J Carlino,Umesh C Karandikar,Sarah E Kralicek,Mary K Estes,Gail A Hecht

Scientific reports 9:3035 PubMed30816200

2019

DGAT1 Inhibitor Suppresses Prostate Tumor Growth and Migration by Regulating Intracellular Lipids and Non-Centrosomal MTOC Protein GM130.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Nardi,Omar E Franco,Philip Fitchev,Alejandro Morales,Renee E Vickman,Simon W Hayward,Susan E Crawford
View all publications
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Product promise

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