Rabbit Polyclonal Claspin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human Claspin.
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- Cancer medicine 10:5574-55882021Overexpression of claspin promotes docetaxel resistance and is associated with prostate-specific antigen recurrence in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakashi Babasaki,Kazuhiro Sentani,Yohei Sekino,Go Kobayashi,Quoc Thang Pham,Narutaka Katsuya,Shintaro Akabane,Daiki Taniyama,Tetsutaro Hayashi,Masaki Shiota,Naohide Oue,Jun Teishima,Akio Matsubara,Wataru YasuiPubMed 34240817
- Genetic testing and molecular biomarkers 25:131-1392021Overexpression Promotes Tumor Progression and Predicts Poor Clinical Outcome in Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Cai,Jiexin Luo,Qinwei Liu,Zezhen Liu,Yan Zhao,Xiangkun Wu,Yuanzhi Yuegao,Yeci Lei,Jianming Lu,Ying Wang,Zhouda Cai,Xiaolu Duan,Ming Lei,Di Gu,Yongda LiuPubMed 33596143
- Cancer science 111:1020-10272020Claspin overexpression is associated with high-grade histology and poor prognosis in renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGo Kobayashi et. al.PubMed 31912588
- Cell reports 29:3708-3725.e52019The Replisome Mediates A-NHEJ Repair of Telomeres Lacking POT1-TPP1 Independently of MRN Function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRekha Rai et. al.PubMed 31825846
- PloS one 11:e01555342016Anti-TGF-β Antibody, 1D11, Ameliorates Glomerular Fibrosis in Mouse Models after the Onset of Proteinuria.
- Cancer research 72:5516-282012RhoJ regulates melanoma chemoresistance by suppressing pathways that sense DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHsiang Ho et. al.PubMed 22971344
- EMBO molecular medicine 2:130-422010Aurora-A expressing tumour cells are deficient for homology-directed DNA double strand-break repair and sensitive to PARP inhibition.Applications:WBReactive species:HumanTony Sourisseau,Despina Maniotis,Afshan McCarthy,Chan Tang,Christopher J Lord,Alan Ashworth,Spiros LinardopoulosPubMed 20373286
- Journal of cell science 123:225-352010CDC6 interaction with ATR regulates activation of a replication checkpoint in higher eukaryotic cells.Applications:WBReactive species:HumanKazumasa Yoshida,Nozomi Sugimoto,Satoko Iwahori,Takashi Yugawa,Mako Narisawa-Saito,Tohru Kiyono,Masatoshi FujitaPubMed 20048340
- Molecular cancer research : MCR 7:1510-62009Prevalence and functional analysis of sequence variants in the ATR checkpoint mediator Claspin.
- Cell cycle (Georgetown, Tex.) 8:1036-432009Claspin and Chk1 regulate replication fork stability by different mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer Scorah,Clare H McGowanPubMed 19270516
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Claspin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HAW4
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Chimpanzee | Predicted |
| Gorilla | Predicted |
| Orangutan | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation (PubMed:12766152, PubMed:15190204, PubMed:15707391, PubMed:16123041). Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins (PubMed:12766152, PubMed:15096610, PubMed:15707391, PubMed:16123041). Also required to maintain normal rates of replication fork progression during unperturbed DNA replication. Binds directly to DNA, with particular affinity for branched or forked molecules and interacts with multiple protein components of the replisome such as the MCM2-7 complex and TIMELESS (PubMed:15226314, PubMed:34694004, PubMed:35585232). Important for initiation of DNA replication, recruits kinase CDC7 to phosphorylate MCM2-7 components (PubMed:27401717).
Alternative names
Claspin, hClaspin, CLSPN
Recommended products
Rabbit Polyclonal Claspin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human Claspin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Claspin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HAW4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Claspin also known as CLSPN is a protein involved in DNA replication and checkpoint control during the cell cycle. Its estimated molecular weight is approximately 133 kDa. Claspin acts as a mediator within the ATR (ATM and Rad3-related) signaling pathway particularly during DNA damage response. The protein is highly expressed in cells with active DNA replication such as in rapidly dividing tissues.
- Biological function summary
Claspin plays a significant role in stabilizing replication forks and assists in the activation of checkpoint kinase 1 (Chk1). It is not an isolated player; instead Claspin functions as part of a larger complex involving ATR and other checkpoint proteins. Through its interactions it helps to enforce a halt in the cell cycle allowing cells to repair DNA and maintain genomic integrity.
- Pathways
Claspin has a critical function in the ATR-Chk1 pathway essential for the DNA damage response and the maintenance of replication fork stability. Claspin interacts with the protein TopBP1 an important player in facilitating ATR activation. It also works alongside proteins such as Rad17 and replication protein A (RPA) all of which play roles in monitoring and responding to DNA replication stress.
- Associated diseases and disorders
Claspin has been linked to cancer particularly in cases where DNA damage response pathways are disrupted. Dysregulation of Claspin can affect the cell’s ability to repair DNA contributing to oncogenesis. Another condition related to Claspin is ataxia-telangiectasia a disorder characterized by total loss of ATM function which can cascade into ineffective DNA damage responses where Claspin’s role becomes compromised.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claspin antibody (ab3720)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Claspin with ab3720 at 1/1000 (1µg/ml). Detection: DAB.
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