AB3721

Anti-Claspin antibody

Name: Anti-Claspin antibody (ab3721) | Abcam
Brand: Abcam Limited
SKU: AB3721
Price: 520 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Claspin antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Claspin.

View Alternative Names

Claspin, hClaspin, CLSPN

2 Images

Supplier Data

Immunoprecipitation - Anti-Claspin antibody (AB3721)

Claspin was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab3721 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab3721 at 0.1μg/mL.

Lane 1 : ab3721 IP in HeLa whole cell lysate.

Lane 2 : ab3721 IP in HeLa whole cell lysate.

Lane 3 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds

All lanes:

Immunoprecipitation - Anti-Claspin antibody (ab3721)

Predicted band size: 151 kDa

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Supplier Data

Western blot - Anti-Claspin antibody (AB3721)

All lanes:

Western blot - Anti-Claspin antibody (ab3721) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK293T whole cell lysate at 50 µg

Predicted band size: 151 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human Claspin. The exact immunogen used to generate this antibody is proprietary information.

Q9HAW4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claspin also known as CLSPN is a protein involved in DNA replication and checkpoint control during the cell cycle. Its estimated molecular weight is approximately 133 kDa. Claspin acts as a mediator within the ATR (ATM and Rad3-related) signaling pathway particularly during DNA damage response. The protein is highly expressed in cells with active DNA replication such as in rapidly dividing tissues.

Biological function summary

Claspin plays a significant role in stabilizing replication forks and assists in the activation of checkpoint kinase 1 (Chk1). It is not an isolated player; instead Claspin functions as part of a larger complex involving ATR and other checkpoint proteins. Through its interactions it helps to enforce a halt in the cell cycle allowing cells to repair DNA and maintain genomic integrity.

Pathways

Claspin has a critical function in the ATR-Chk1 pathway essential for the DNA damage response and the maintenance of replication fork stability. Claspin interacts with the protein TopBP1 an important player in facilitating ATR activation. It also works alongside proteins such as Rad17 and replication protein A (RPA) all of which play roles in monitoring and responding to DNA replication stress.

Claspin has been linked to cancer particularly in cases where DNA damage response pathways are disrupted. Dysregulation of Claspin can affect the cell’s ability to repair DNA contributing to oncogenesis. Another condition related to Claspin is ataxia-telangiectasia a disorder characterized by total loss of ATM function which can cascade into ineffective DNA damage responses where Claspin’s role becomes compromised.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation (PubMed : 12766152, PubMed : 15190204, PubMed : 15707391, PubMed : 16123041). Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins (PubMed : 12766152, PubMed : 15096610, PubMed : 15707391, PubMed : 16123041). Also required to maintain normal rates of replication fork progression during unperturbed DNA replication. Binds directly to DNA, with particular affinity for branched or forked molecules and interacts with multiple protein components of the replisome such as the MCM2-7 complex and TIMELESS (PubMed : 15226314, PubMed : 34694004, PubMed : 35585232). Important for initiation of DNA replication, recruits kinase CDC7 to phosphorylate MCM2-7 components (PubMed : 27401717).

See full target information Claspin

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113520 PubMed41069850

2025

ALKBH5-IGF2BP2 axis mediates prostate cancer progression and docetaxel resistance via m6A-stabilized CLSPN RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Yutong Chen,Yang Li,Zongzhu Liang,Zhao Yang,Haotian Ren,Wanqing Wei,Yuanjie Niu,Zhiqun Shang

The Journal of biological chemistry 278:25207-17 PubMed12676962

2003

Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on Ser-345.

Applications

Unspecified application

Species

Unspecified reactive species

Kecheng Jiang,Elizabeth Pereira,Melissa Maxfield,Beatriz Russell,Dawn Marie Goudelock,Yolanda Sanchez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Claspin antibody

Immunoprecipitation - Anti-Claspin antibody (AB3721)

Western blot - Anti-Claspin antibody (AB3721)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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