Rabbit Polyclonal Clathrin heavy chain antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 145 publications.
Images
Publications
Filter by
- Nature biomedical engineering 8:291-3092023High-throughput screening of genetic and cellular drivers of syncytium formation induced by the spike protein of SARS-CoV-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharles W F Chan,Bei Wang,Lang Nan,Xiner Huang,Tianjiao Mao,Hoi Yee Chu,Cuiting Luo,Hin Chu,Gigi C G Choi,Ho Cheung Shum,Alan S L WongPubMed 37996617
- Nature communications 14:61902023MYL3 protects chondrocytes from senescence by inhibiting clathrin-mediated endocytosis and activating of Notch signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHe Cao,Panpan Yang,Jia Liu,Yan Shao,Honghao Li,Pinglin Lai,Hong Wang,Anling Liu,Bin Guo,Yujin Tang,Xiaochun Bai,Kai LiPubMed 37794006
- Nature methods 20:1183-11862023Open-3DSIM: an open-source three-dimensional structured illumination microscopy reconstruction platform.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuijie Cao,Yaning Li,Xin Chen,Xichuan Ge,Meiqi Li,Meiling Guan,Yiwei Hou,Yunzhe Fu,Xinzhu Xu,Christophe Leterrier,Shan Jiang,Baoxiang Gao,Peng XiPubMed 37474809
- Biomaterials science 11:4210-42252023Effectiveness of a novel gene nanotherapy based on putrescine for cancer treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaínza Lores,Manuel Gámez-Chiachio,María Cascallar,Carmen Ramos-Nebot,Pablo Hurtado,Sandra Alijas,Rafael López López,Roberto Piñeiro,Gema Moreno-Bueno,María de la FuentePubMed 36790445
- Microbiology spectrum 11:e04753222023MARCO Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection through Intensifying Viral GP5-Induced Apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxiao Zhang,Yongjie Chen,Songbei Li,Jinling Wang,Zhan He,Jiecong Yan,Xiaohong Liu,Chunhe GuoPubMed 37078873
- The Journal of biological chemistry 299:1046962023An A-kinase anchoring protein (ACBD3) coordinates traffic-induced PKA activation at the Golgi.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Jia,Shuocheng Tang,Xihua Yue,Shuaiyang Jing,Lianhui Zhu,Chuanting Tan,Jingkai Gao,Yulei Du,Intaek Lee,Yi QianPubMed 37044218
- Histochemistry and cell biology 159:489-5002023Opposite changes in the expression of clathrin and caveolin-1 in normal and cancerous human prostate tissue: putative clathrin-mediated recycling of EGFR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBoyu Xie,Hawra Zuhair,Rui Henrique,Michael Millar,Timothy Robson,Christopher Thrasivoulou,Kerry Dickens,Jane Pendjiky,Asif Muneer,Hiten Patel,Aamir AhmedPubMed 36869937
- The Journal of cell biology 222:2023Clathrin coats partially preassemble and subsequently bend during endocytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarkus Mund,Aline Tschanz,Yu-Le Wu,Felix Frey,Johanna L Mehl,Marko Kaksonen,Ori Avinoam,Ulrich S Schwarz,Jonas RiesPubMed 36734980
- Proceedings of the National Academy of Sciences of the United States of America 120:e22113681202023Clathrin mediates both internalization and vesicular release of triggered T cell receptor at the immunological synapse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAudun Kvalvaag,Salvatore Valvo,Pablo F Céspedes,David G Saliba,Elke Kurz,Kseniya Korobchevskaya,Michael L DustinPubMed 36730202
- Methods in molecular biology (Clifton, N.J.) 2557:619-6332022Immunoprecipitation and Western Blot Analysis of AP-1 Clathrin-Coated Vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMargaret Kell,Abby Halpern,Heike FölschPubMed 36512241
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| ICC/IF | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
| Chicken | Predicted | Predicted |
| Hamster | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Hamster | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Hamster | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582). The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825). Plays a role in early autophagosome formation (PubMed:20639872).
Alternative names
Clathrin heavy chain 1, Clathrin heavy chain on chromosome 17, CLH-17, CLH17, CLTCL2, KIAA0034, CLTC
Recommended products
Rabbit Polyclonal Clathrin heavy chain antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 145 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
Replenishment batches of our polyclonal antibody, ab21679 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab172958.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Western blot - Anti-Clathrin heavy chain antibody (ab21679)
Blocking buffer: 2% BSA
Gel type: TA
Exposure Time: 1 minute
All lanes: Western blot - Anti-Clathrin heavy chain antibody (ab21679) at 1 µg/mL
Lane 1: HeLa whole cell lysate at 10 µg
Lane 2: A431 whole cell lysate at 10 µg
Lane 3: NIH 3T3 whole cell lysate at 10 µg
Lane 4: PC12 whole cell lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 191 kDa
Observed band size: 180 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody (ab21679)
ab21679 staining Clathrin heavy chain in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab21679 at 1μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
This image is courtesy of an anonymous customer reviewWestern blot - Anti-Clathrin heavy chain antibody (ab21679)
All lanes: Western blot - Anti-Clathrin heavy chain antibody (ab21679) at 1/1000 dilution
All lanes: HUVEC cell lysate at 20 µg
SecondaryAll lanes: HRP conjugated goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 191 kDa
Exposure time: 5s
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody (ab21679)
Immunocytochemical analysis of mouse bEnd.3 labeling Clathrin heavy chain with ab21679 at 1/300 dilution using secondary anti-rabbit-alexa fluor 488 at 1/500 dilution.
This image is a courtesy of Anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody (ab21679)
ab21679 staining Clathrin heavy chain in mouse embryonic fibroblasts by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with -20°C ethanol. Samples were incubated with primary antibody at 1/500 dilution (in 0.1% Saponin/1% BSA/PBS) for 1 hour. An Alexa Fluor® 546-conjugated goat polyclonal to rabbit IgG (H+L) was used as secondary antibody at 1/500 dilution. Green color in image show positive staining with Alexa Fluor® 546 conjugated secondary.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com