AB172958

Anti-Clathrin heavy chain antibody [EPR12235(B)]

20ul selling size
RabMAb
Recombinant
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(10 Publications)

Rabbit Recombinant Monoclonal Clathrin heavy chain antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 10 publications.

View Alternative Names

CLH17, CLTCL2, KIAA0034, CLTC, Clathrin heavy chain 1, Clathrin heavy chain on chromosome 17, CLH-17

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (AB172958)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (AB172958)

Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling Clathrin heavy chain with ab172958 at 1/50 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (AB172958)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Clathrin heavy chain with ab172958 at 1/50 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (AB172958)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (AB172958)

Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) labeling Clathrin heavy chain with ab172958 at 1/250 (4.1 μg/mL). ab150077, Alexa Fluor^® 488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/mL) was used as counterstain antibody. Nuclei were stained blue with DAPI.

Confocal image showing cytoplasmic staining in A431 cells.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (AB172958)

Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) labeling Clathrin heavy chain with ab172958 at 1/250 (4.1 μg/mL). ab150077, AlexaFluor^®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/mL) was used as counterstain antibody. Nuclei were stained blue with DAPI.

Confocal image showing cytoplasmic staining in NIH/3T3 cells.

Supplier Data

Western blot - Anti-Clathrin heavy chain antibody [EPR12235(B)] (AB172958)

All lanes:

Western blot - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958) at 1/10000 dilution

Lane 1:

Hela cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 191 kDa

false

Carrier free

Anti-Clathrin heavy chain antibody [EPR12235(B)] - BSA and Azide free
660 APC

APC Anti-Clathrin heavy chain antibody [EPR12235(B)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Clathrin heavy chain antibody [EPR12235(B)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Clathrin heavy chain antibody [EPR12235(B)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Clathrin heavy chain antibody [EPR12235(B)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Clathrin heavy chain antibody [EPR12235(B)]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Clathrin heavy chain antibody [EPR12235(B)]
578 PE

PE Anti-Clathrin heavy chain antibody [EPR12235(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12235(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Clathrin heavy chain also known as clathrin heavy chain 1 or heavy chain kDa is a fundamental component of the clathrin protein complex with a molecular weight commonly around 180 kDa. This chain-like protein mechanically assembles into a triskelion shape consisting of three heavy chains and three light chains allowing it to form a lattice structure that coats vesicles. Clathrin heavy chain is expressed widely especially in cells with high endocytic and vesicle transport activity like neuronal and epithelial cells. It plays a pivotal role in cellular trafficking by driving the formation of clathrin-coated pits and vesicles.

Biological function summary

Functions of the clathrin heavy chain include mediating the endocytosis of receptors and other membrane proteins contributing to intracellular sorting and vesicle trafficking. It is a part of the clathrin triskelion complex which is essential for maintaining proper cellular homeostasis. The clathrin lattice structure dynamically assembles and disassembles to facilitate the transport of molecules from the plasma membrane to the endosomes influencing endocytic and post-Golgi network trafficking processes. This protein's ability to form complexes enables efficient cargo selection and vesicle scission.

Pathways

Clathrin-mediated endocytosis serves as a critical pathway for internalizing substances and is fundamental in the regulation of signal transduction pathways and receptor recycling. The clathrin heavy chain interacts closely with proteins such as adaptins and dynamin in these pathways to ensure accurate vesicle budding and trafficking. In synaptic vesicle recycling clathrin collaborates with proteins like synaptotagmins to manage neurotransmitter release maintaining synaptic transmission and neuronal function.

Disruptions in clathrin heavy chain function can lead to conditions such as neurodegenerative diseases and cancer. For instance alterations in clathrin-mediated endocytosis can affect receptor trafficking and signaling contributing to Alzheimer’s disease. Similarly aberrant clathrin function can influence cell cycle control and apoptosis pathways involved in cancer progression. In cancer the regulation of the epidermal growth factor receptor (EGFR) by clathrin-dependent endocytosis highlights the complex interplay between clathrin and oncogenic signaling pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed : 15858577, PubMed : 16968737, PubMed : 21297582). The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed : 23532825). Plays a role in early autophagosome formation (PubMed : 20639872). Interaction with DNAJC6 mediates the recruitment of HSPA8 to the clathrin lattice and creates local destabilization of the lattice promoting uncoating (By similarity).

See full target information CLTC

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 223: PubMed38578286

2024

The role of the AP-1 adaptor complex in outgoing and incoming membrane traffic.

Applications

Unspecified application

Species

Unspecified reactive species

Margaret S Robinson,Robin Antrobus,Anneri Sanger,Alexandra K Davies,David C Gershlick

Scientific reports 12:16477 PubMed36183038

2022

Enamel defects in Acp4 mice and human ACP4 mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Liang,Shih-Kai Wang,Charles Smith,Hong Zhang,Yuanyuan Hu,Figen Seymen,Mine Koruyucu,Yelda Kasimoglu,Jung-Wook Kim,Chuhua Zhang,Thomas L Saunders,James P Simmer,Jan C-C Hu

Advanced drug delivery reviews 188:114403 PubMed35777667

2022

Nanoparticle entry into cells; the cell biology weak link.

Applications

Unspecified application

Species

Unspecified reactive species

Gareth Griffiths,Jean Gruenberg,Mark Marsh,Jens Wohlmann,Arwyn T Jones,Robert G Parton

Science advances 8:eabm4006 PubMed35507653

2022

Expansion microscopy with ninefold swelling (NIFS) hydrogel permits cellular ultrastructure imaging on conventional microscope.

Applications

Unspecified application

Species

Unspecified reactive species

Hongxia Li,Antony R Warden,Jie He,Guangxia Shen,Xianting Ding

BioFactors (Oxford, England) 48:67-85 PubMed34866251

2021

22-kD growth hormone-induced nuclear GHR/STAT5/CyclinD1 signaling pathway plays an important role in promoting mesenchymal stem cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei V Zheng,Yaqin Li,Yanwei Xu,Tao Zhou,Dezhi Li,Xianyi Cheng,Yu Xiong,Shaobin Wang,Zaizhong Chen

Development (Cambridge, England) 147: PubMed33144400

2020

Ontogenesis of the tear drainage system requires Prickle1-driven polarized basement membrane deposition.

Applications

Unspecified application

Species

Unspecified reactive species

Dianlei Guo,Jiali Ru,Fuxiang Mao,Hong Ouyang,Rong Ju,Kaili Wu,Yizhi Liu,Chunqiao Liu

Cell cycle (Georgetown, Tex.) 19:391-404 PubMed31916488

2020

METTL3-mediated mA is required for murine oocyte maturation and maternal-to-zygotic transition.

Applications

Unspecified application

Species

Unspecified reactive species

Xuesong Sui,Yue Hu,Chao Ren,Qiqi Cao,Shuai Zhou,Yumeng Cao,Mingrui Li,Wenjie Shu,Ran Huo

PLoS pathogens 15:e1007938 PubMed31356638

2019

Endocytosis of flavivirus NS1 is required for NS1-mediated endothelial hyperpermeability and is abolished by a single N-glycosylation site mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Chunling Wang,Henry Puerta-Guardo,Scott B Biering,Dustin R Glasner,Edwina B Tran,Mark Patana,Trent A Gomberg,Carmel Malvar,Nicholas T N Lo,Diego A Espinosa,Eva Harris

The Journal of biological chemistry 293:19710-19724 PubMed30361437

2018

Oligomerization of Frizzled and LRP5/6 protein initiates intracellular signaling for the canonical WNT/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Hua,Yilin Yang,Qian Li,Xinyu He,Wei Zhu,Jiyong Wang,Xiaoqing Gan

Scientific reports 7:4572 PubMed28676702

2017

Extracellular matrix promotes clathrin-dependent endocytosis of prolactin and STAT5 activation in differentiating mammary epithelial cells.

Applications

Species

Unspecified reactive species

Rebecca E Bridgewater,Charles H Streuli,Patrick T Caswell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Clathrin heavy chain antibody [EPR12235(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (AB172958)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (AB172958)

Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (AB172958)

Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (AB172958)

Western blot - Anti-Clathrin heavy chain antibody [EPR12235(B)] (AB172958)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise