Rabbit Recombinant Monoclonal Clathrin heavy chain antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (AB305260), expandable thumbnail](https://content.abcam.com/products/images/clathrin-heavy-chain-antibody-epr24236-222-bsa-and-azide-free-ab305260--immunocytochemistry-immunofluorescence-img403905.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (AB305260)")
![Immunoprecipitation - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (AB305260), expandable thumbnail](https://content.abcam.com/products/images/clathrin-heavy-chain-antibody-epr24236-222-bsa-and-azide-free-ab305260--immunoprecipitation-img404428.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (AB305260)")
![Western blot - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (AB305260), expandable thumbnail](https://content.abcam.com/products/images/clathrin-heavy-chain-antibody-epr24236-222-bsa-and-azide-free-ab305260--western-blot-img404120.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (AB305260)")
![Flow Cytometry (Intracellular) - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (AB305260), expandable thumbnail](https://content.abcam.com/products/images/clathrin-heavy-chain-antibody-epr24236-222-bsa-and-azide-free-ab305260--flow-cytometry-intracellular-img403924.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (AB305260)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582). The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825). Plays a role in early autophagosome formation (PubMed:20639872). Interaction with DNAJC6 mediates the recruitment of HSPA8 to the clathrin lattice and creates local destabilization of the lattice promoting uncoating (By similarity).
Alternative names
CLH17, CLTCL2, KIAA0034, CLTC, Clathrin heavy chain 1, Clathrin heavy chain on chromosome 17, CLH-17
Recommended products
Rabbit Recombinant Monoclonal Clathrin heavy chain antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24236-222
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Clathrin heavy chain also known as clathrin heavy chain 1 or heavy chain kDa is a fundamental component of the clathrin protein complex with a molecular weight commonly around 180 kDa. This chain-like protein mechanically assembles into a triskelion shape consisting of three heavy chains and three light chains allowing it to form a lattice structure that coats vesicles. Clathrin heavy chain is expressed widely especially in cells with high endocytic and vesicle transport activity like neuronal and epithelial cells. It plays a pivotal role in cellular trafficking by driving the formation of clathrin-coated pits and vesicles.
- Biological function summary
Functions of the clathrin heavy chain include mediating the endocytosis of receptors and other membrane proteins contributing to intracellular sorting and vesicle trafficking. It is a part of the clathrin triskelion complex which is essential for maintaining proper cellular homeostasis. The clathrin lattice structure dynamically assembles and disassembles to facilitate the transport of molecules from the plasma membrane to the endosomes influencing endocytic and post-Golgi network trafficking processes. This protein's ability to form complexes enables efficient cargo selection and vesicle scission.
- Pathways
Clathrin-mediated endocytosis serves as a critical pathway for internalizing substances and is fundamental in the regulation of signal transduction pathways and receptor recycling. The clathrin heavy chain interacts closely with proteins such as adaptins and dynamin in these pathways to ensure accurate vesicle budding and trafficking. In synaptic vesicle recycling clathrin collaborates with proteins like synaptotagmins to manage neurotransmitter release maintaining synaptic transmission and neuronal function.
- Associated diseases and disorders
Disruptions in clathrin heavy chain function can lead to conditions such as neurodegenerative diseases and cancer. For instance alterations in clathrin-mediated endocytosis can affect receptor trafficking and signaling contributing to Alzheimer’s disease. Similarly aberrant clathrin function can influence cell cycle control and apoptosis pathways involved in cancer progression. In cancer the regulation of the epidermal growth factor receptor (EGFR) by clathrin-dependent endocytosis highlights the complex interplay between clathrin and oncogenic signaling pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (ab305260), expandable thumbnail](https://content.abcam.com/products/images/clathrin-heavy-chain-antibody-epr24236-222-bsa-and-azide-free-ab305260--immunocytochemistry-immunofluorescence-img403905.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (ab305260)")
Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (ab305260)
This data was developed using Anti-Clathrin heavy chain antibody [EPR24236-222] ab305259, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Clathrin heavy chain with Anti-Clathrin heavy chain antibody [EPR24236-222] ab305259 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing punctuated cytoplasmic staining in HeLa cell line. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red).
The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.![Immunoprecipitation - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (ab305260), expandable thumbnail](https://content.abcam.com/products/images/clathrin-heavy-chain-antibody-epr24236-222-bsa-and-azide-free-ab305260--immunoprecipitation-img404428.jpg "Immunoprecipitation - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (ab305260)")
Immunoprecipitation - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (ab305260)
This data was developed using Anti-Clathrin heavy chain antibody [EPR24236-222] ab305259, the same antibody clone in a different buffer formulation.
Clathrin heavy chain was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with Anti-Clathrin heavy chain antibody [EPR24236-222] ab305259 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Clathrin heavy chain antibody [EPR24236-222] ab305259. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg
Lane 2: Anti-Clathrin heavy chain antibody [EPR24236-222] ab305259 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Clathrin heavy chain antibody [EPR24236-222] ab305259 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 50 secondsAll lanes: Immunoprecipitation - Anti-Clathrin heavy chain antibody [EPR24236-222] (Anti-Clathrin heavy chain antibody [EPR24236-222] ab305259) at 1/30 dilution
All lanes: HeLa (human cervix adenocarcinoma epithelial cell)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 180 kDa
Exposure time: 50s
![Western blot - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (ab305260), expandable thumbnail](https://content.abcam.com/products/images/clathrin-heavy-chain-antibody-epr24236-222-bsa-and-azide-free-ab305260--western-blot-img404120.jpg "Western blot - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (ab305260)")
Western blot - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (ab305260)
This data was developed using Anti-Clathrin heavy chain antibody [EPR24236-222] ab305259, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 37 secondsAll lanes: Western blot - Anti-Clathrin heavy chain antibody [EPR24236-222] (Anti-Clathrin heavy chain antibody [EPR24236-222] ab305259) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/2000 dilution
Observed band size: 180 kDa
Exposure time: 37s
![Flow Cytometry (Intracellular) - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (ab305260), expandable thumbnail](https://content.abcam.com/products/images/clathrin-heavy-chain-antibody-epr24236-222-bsa-and-azide-free-ab305260--flow-cytometry-intracellular-img403924.jpg "Flow Cytometry (Intracellular) - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (ab305260)")
Flow Cytometry (Intracellular) - Anti-Clathrin heavy chain antibody [EPR24236-222] - BSA and Azide free (ab305260)
This data was developed using Anti-Clathrin heavy chain antibody [EPR24236-222] ab305259, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Clathrin heavy chain with Anti-Clathrin heavy chain antibody [EPR24236-222] ab305259 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
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