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AB24578

Anti-Clathrin heavy chain antibody [TD.1]

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(11 Publications)

Mouse Monoclonal Clathrin heavy chain antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human CLTC.

View Alternative Names

CLH17, CLTCL2, KIAA0034, CLTC, Clathrin heavy chain 1, Clathrin heavy chain on chromosome 17, CLH-17

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (AB24578)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (AB24578)

ab24578 at a 1/200 dilution staining clathrin triskelion (blue) throughout the methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence. The Golgi is brilliantly stained by Giantin (yellow). Secondary antibodies used were a goat anti-mouse alexafluor AF546 and a goat anti-rabbit alexafluor AF488 applied at 1 ug/mL. The DNA was visualized with DAPI at 1 ug/mL. The colors of this image were altered.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TD.1

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human CLTC. The exact immunogen used to generate this antibody is proprietary information.

Q00610

Specificity

This antibody reacts with clathrin heavy chains (CHC).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.03% Thimerosal (merthiolate)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Clathrin heavy chain also known as clathrin heavy chain 1 or heavy chain kDa is a fundamental component of the clathrin protein complex with a molecular weight commonly around 180 kDa. This chain-like protein mechanically assembles into a triskelion shape consisting of three heavy chains and three light chains allowing it to form a lattice structure that coats vesicles. Clathrin heavy chain is expressed widely especially in cells with high endocytic and vesicle transport activity like neuronal and epithelial cells. It plays a pivotal role in cellular trafficking by driving the formation of clathrin-coated pits and vesicles.
Biological function summary

Functions of the clathrin heavy chain include mediating the endocytosis of receptors and other membrane proteins contributing to intracellular sorting and vesicle trafficking. It is a part of the clathrin triskelion complex which is essential for maintaining proper cellular homeostasis. The clathrin lattice structure dynamically assembles and disassembles to facilitate the transport of molecules from the plasma membrane to the endosomes influencing endocytic and post-Golgi network trafficking processes. This protein's ability to form complexes enables efficient cargo selection and vesicle scission.

Pathways

Clathrin-mediated endocytosis serves as a critical pathway for internalizing substances and is fundamental in the regulation of signal transduction pathways and receptor recycling. The clathrin heavy chain interacts closely with proteins such as adaptins and dynamin in these pathways to ensure accurate vesicle budding and trafficking. In synaptic vesicle recycling clathrin collaborates with proteins like synaptotagmins to manage neurotransmitter release maintaining synaptic transmission and neuronal function.

Disruptions in clathrin heavy chain function can lead to conditions such as neurodegenerative diseases and cancer. For instance alterations in clathrin-mediated endocytosis can affect receptor trafficking and signaling contributing to Alzheimer’s disease. Similarly aberrant clathrin function can influence cell cycle control and apoptosis pathways involved in cancer progression. In cancer the regulation of the epidermal growth factor receptor (EGFR) by clathrin-dependent endocytosis highlights the complex interplay between clathrin and oncogenic signaling pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed : 15858577, PubMed : 16968737, PubMed : 21297582). The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed : 23532825). Plays a role in early autophagosome formation (PubMed : 20639872). Interaction with DNAJC6 mediates the recruitment of HSPA8 to the clathrin lattice and creates local destabilization of the lattice promoting uncoating (By similarity).
See full target information CLTC
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7522 PubMed36473871

2022

The dynamic clustering of insulin receptor underlies its signaling and is disrupted in insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandra Dall'Agnese,Jesse M Platt,Ming M Zheng,Max Friesen,Giuseppe Dall'Agnese,Alyssa M Blaise,Jessica B Spinelli,Jonathan E Henninger,Erin N Tevonian,Nancy M Hannett,Charalampos Lazaris,Hannah K Drescher,Lea M Bartsch,Henry R Kilgore,Rudolf Jaenisch,Linda G Griffith,Ibrahim I Cisse,Jacob F Jeppesen,Tong I Lee,Richard A Young

The Journal of membrane biology 255:591-597 PubMed35226119

2022

Protein-Protein Interactions on Membrane Surfaces Analysed Using Pull-Downs with Supported Bilayers on Silica Beads.

Applications

Unspecified application

Species

Unspecified reactive species

Devika S Andhare,Himani Khurana,Thomas J Pucadyil

Cells 10: PubMed33672612

2021

Role of Clathrin Light Chains in Regulating Invadopodia Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Mukenhirn,Francesco Muraca,Delia Bucher,Edgar Asberger,Elisa Cappio Barazzone,Elisabetta Ada Cavalcanti-Adam,Steeve Boulant

Cell reports 30:2594-2602.e3 PubMed32101738

2020

Vesicle Clustering in a Living Synapse Depends on a Synapsin Region that Mediates Phase Separation.

Applications

Unspecified application

Species

Unspecified reactive species

Arndt Pechstein,Nikolay Tomilin,Kristin Fredrich,Olga Vorontsova,Elena Sopova,Emma Evergren,Volker Haucke,Lennart Brodin,Oleg Shupliakov

The Journal of cell biology 205:377-93 PubMed24798732

2014

Actin scaffolding by clathrin heavy chain is required for skeletal muscle sarcomere organization.

Applications

Unspecified application

Species

Unspecified reactive species

Stéphane Vassilopoulos,Christel Gentil,Jeanne Lainé,Pierre-Olivier Buclez,Agathe Franck,Arnaud Ferry,Guillaume Précigout,Robyn Roth,John E Heuser,Frances M Brodsky,Luis Garcia,Gisèle Bonne,Thomas Voit,France Piétri-Rouxel,Marc Bitoun

The Journal of neuroscience : the official journal of the Society for Neuroscience 33:4151-64 PubMed23447623

2013

Tyrosine phosphorylation regulates the endocytosis and surface expression of GluN3A-containing NMDA receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Dhrubajyoti Chowdhury,Sonia Marco,Ian M Brooks,Aitor Zandueta,Yijian Rao,Volker Haucke,John F Wesseling,Steven J Tavalin,Isabel Pérez-Otaño

Molecular cancer research : MCR 9:688-701 PubMed21571834

2011

COP35, a cholangiocarcinoma-binding oligopeptide, interacts with the clathrin heavy chain accompanied by GRP78.

Applications

IHC-P

Species

Human

Hiroe Kitahara,Junya Masumoto,Alan L Parker,Fukuto Maruta,Naoki Kubo,Akira Shimizu,Noriyuki Akita,Shiro Miwa,Naoya Kobayashi,Jun Nakayama,Shinichi Miyagawa

The Journal of neuroscience : the official journal of the Society for Neuroscience 28:10257-71 PubMed18842885

2008

Clathrin assembly protein AP180 and CALM differentially control axogenesis and dendrite outgrowth in embryonic hippocampal neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Ittai Bushlin,Ronald S Petralia,Fangbai Wu,Asaff Harel,Mohamed R Mughal,Mark P Mattson,Pamela J Yao

PloS one 3:e3115 PubMed18769625

2008

Clathrin is spindle-associated but not essential for mitosis.

Applications

WB

Species

Human

Joana Borlido,Greg Veltri,Antony P Jackson,Ian G Mills

Biochimica et biophysica acta 1783:1129-39 PubMed18261469

2008

A presynaptically toxic secreted phospholipase A2 is internalized into motoneuron-like cells where it is rapidly translocated into the cytosol.

Applications

Unspecified application

Species

Unspecified reactive species

Zala Jenko Praznikar,Lidija Kovacic,Edward G Rowan,Rok Romih,Paola Rusmini,Angelo Poletti,Igor Krizaj,Joze Pungercar
View all publications
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