AB103553

Anti-Clathrin light chain antibody

Name: Anti-Clathrin light chain antibody (ab103553) | Abcam
Brand: Abcam Limited
SKU: AB103553
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Clathrin light chain antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Clathrin light chain A.

View Alternative Names

Clathrin light chain A, Lca, CLTA

3 Images

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Western blot - Anti-Clathrin light chain antibody (AB103553)

All lanes:

Western blot - Anti-Clathrin light chain antibody (ab103553) at 1/500 dilution

All lanes:

Mouse spleen tissue lysate at 25 µg

Predicted band size: 27 kDa

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Western blot - Anti-Clathrin light chain antibody (AB103553)

All lanes:

Western blot - Anti-Clathrin light chain antibody (ab103553) at 1/500 dilution

Lane 1:

Clathrin light chain transfected 293T cell line lysate at 25 µg

Lane 2:

Non-transfected 293T cell line lysate at 25 µg

Predicted band size: 16 kDa,19 kDa,21 kDa,23 kDa,27 kDa,35 kDa

Observed band size: 14 kDa,16 kDa,18 kDa

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Western blot - Anti-Clathrin light chain antibody (AB103553)

All lanes:

Western blot - Anti-Clathrin light chain antibody (ab103553) at 1/500 dilution

All lanes:

Human stomach tissue lysate at 25 µg

Predicted band size: 27 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Clathrin light chain A.

P09496

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Clathrin light chain also known as clathrin LCa and LCb is a low-molecular-weight component of the clathrin protein complex. It plays a mechanical role in the processes that drive clathrin-mediated endocytosis. Each clathrin triskelion the basic unit of the clathrin coat comprises three clathrin heavy chains and three clathrin light chains. The mass of clathrin light chain is approximately 25-29 kDa. This protein is expressed in various tissues throughout the body with notable presence in the central nervous system and liver.

Biological function summary

Clathrin light chain modulates the lattice dynamics by interacting with clathrin heavy chains in a clathrin protein complex. This interaction supports the stability and assembly of the clathrin lattice affecting the efficiency and specificity of endocytic vesicle formation. Clathrin light chain acts in concert with other accessory proteins to regulate clathrin coat assembly and disassembly playing a dynamic role in cellular trafficking processes.

Pathways

The clathrin light chain participates in essential cellular pathways such as the clathrin-mediated endocytosis and synaptic vesicle recycling pathways. These pathways facilitate the internalization and recycling of membrane proteins and lipids ensuring cellular homeostasis. In these processes the clathrin light chain works closely with related proteins such as dynamin and adaptin to control vesicle scission and cargo selection.

Abnormalities in clathrin light chain function connect to neurodegenerative disorders like Huntington's disease and certain types of cancers. In Huntington's disease disrupted endocytic pathways that involve clathrin light chain impact cellular communication and nutrient uptake. Cancer cells may exhibit altered expression or function of clathrin light chain affecting pathways important for cell proliferation and survival. The clathrin light chain can interact with the huntingtin protein in these contexts influencing disease progression and cellular dynamics.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed : 15858577, PubMed : 21297582).

See full target information Clathrin light chain A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biochimica et biophysica acta. Molecular and cell 1863:834-843 PubMed29673706

2018

Lysophosphatidylcholine acyltransferase 3 deficiency impairs 3T3L1 cell adipogenesis through activating Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Feng,Bin Lou,Jibin Dong,Zhiqiang Li,Yunqin Chen,Yue Li,Xuemei Zhang,Xian-Cheng Jiang,Tingbo Ding

Proteomics. Clinical applications 9:817-31 PubMed25921334

2015

The protein interactome of collapsin response mediator protein-2 (CRMP2/DPYSL2) reveals novel partner proteins in brain tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Martins-de-Souza,Juliana S Cassoli,Juliana M Nascimento,Kenneth Hensley,Paul C Guest,Andres M Pinzon-Velasco,Christoph W Turck

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Clathrin light chain antibody

Western blot - Anti-Clathrin light chain antibody (AB103553)

Western blot - Anti-Clathrin light chain antibody (AB103553)

Western blot - Anti-Clathrin light chain antibody (AB103553)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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