Mouse Monoclonal Clathrin light chain antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Clathrin light chain A.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin light chain antibody [CLC/1421] - BSA and Azide free (AB269758), expandable thumbnail](https://content.abcam.com/products/images/clathrin-light-chain-antibody-clc-1421-bsa-and-azide-free-ab269758--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15542.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin light chain antibody [CLC/1421] - BSA and Azide free (AB269758)")
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human Clathrin light chain A. The exact immunogen used to generate this antibody is proprietary information. Database link P09496
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Version
3 products for Alternative Product
Target data
Function
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:21297582).
Targets
Clathrin light chain B
Alternative names
Clathrin light chain A, Lca, CLTA
Recommended products
Mouse Monoclonal Clathrin light chain antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Clathrin light chain A.
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human Clathrin light chain A. The exact immunogen used to generate this antibody is proprietary information. Database link P09496
- Carrier free
Yes
- Clone number
CLC/1421
- Purification technique
Affinity purification Protein A/G
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
ab269758 is a carrier free version of Anti-Clathrin light chain antibody [CLC/1421] ab219298.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Clathrin light chain also known as clathrin LCa and LCb is a low-molecular-weight component of the clathrin protein complex. It plays a mechanical role in the processes that drive clathrin-mediated endocytosis. Each clathrin triskelion the basic unit of the clathrin coat comprises three clathrin heavy chains and three clathrin light chains. The mass of clathrin light chain is approximately 25-29 kDa. This protein is expressed in various tissues throughout the body with notable presence in the central nervous system and liver.
- Biological function summary
Clathrin light chain modulates the lattice dynamics by interacting with clathrin heavy chains in a clathrin protein complex. This interaction supports the stability and assembly of the clathrin lattice affecting the efficiency and specificity of endocytic vesicle formation. Clathrin light chain acts in concert with other accessory proteins to regulate clathrin coat assembly and disassembly playing a dynamic role in cellular trafficking processes.
- Pathways
The clathrin light chain participates in essential cellular pathways such as the clathrin-mediated endocytosis and synaptic vesicle recycling pathways. These pathways facilitate the internalization and recycling of membrane proteins and lipids ensuring cellular homeostasis. In these processes the clathrin light chain works closely with related proteins such as dynamin and adaptin to control vesicle scission and cargo selection.
- Associated diseases and disorders
Abnormalities in clathrin light chain function connect to neurodegenerative disorders like Huntington's disease and certain types of cancers. In Huntington's disease disrupted endocytic pathways that involve clathrin light chain impact cellular communication and nutrient uptake. Cancer cells may exhibit altered expression or function of clathrin light chain affecting pathways important for cell proliferation and survival. The clathrin light chain can interact with the huntingtin protein in these contexts influencing disease progression and cellular dynamics.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin light chain antibody [CLC/1421] - BSA and Azide free (ab269758), expandable thumbnail](https://content.abcam.com/products/images/clathrin-light-chain-antibody-clc-1421-bsa-and-azide-free-ab269758--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15542.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin light chain antibody [CLC/1421] - BSA and Azide free (ab269758)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin light chain antibody [CLC/1421] - BSA and Azide free (ab269758)
Immunohistochemical analysis of paraffin embedded, formalin fixed Human pancreas tissue labeling Clathrin light chain using Anti-Clathrin light chain antibody [CLC/1421] ab219298 at 1 µg/ml.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Clathrin light chain antibody [CLC/1421] ab219298).
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