AB307693

Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
KO Validated
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Knockout Tested Rabbit Recombinant Monoclonal Claudin 1 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, IP and reacts with Human, Mouse, Rat samples.

View Alternative Names

Claudin-1, Cldn1

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

This data was developed using ab307692, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma labeling Claudin 1 with ab307692 at 1/100 followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Membranous staining on human gastric adenocarcinoma (PMID : 24535143). The section was incubated with ab307692 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

This data was developed using ab307692, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CLDN1 KO A431(CLDN1 knockout human epidermoid carcinoma epithelial cell) cells labelling Claudin 1 with ab307692 at 1/250 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) antibody at 1/1000 dilution (Green).

Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) ( ab195889 ) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is AlexaFluor488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

Confocal image showing membranous and cytoplasmic staining in wildtype A431 cells and showing no staining in CLDN1 knockout A431 cells.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

This data was developed using ab307692, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Claudin 1 with ab307692 at 1/100 followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Membranous staining on human colon (PMID : 25038829). The section was incubated with ab307692 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

This data was developed using ab307692, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) Parental A431 (h tissue labeling Claudin 1 with ab307692 at 1/500 (1.028 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on parental A431 (human epidermal carcinoma cells) cell pellet, negative staining on CLDN1 knockout A431 cell pellet (ab261889). The section was incubated with ab307692 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

This data was developed using ab307692, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling Claudin 1 with ab307692 at 1/100 followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Membranous staining on mouse breast cancer. The section was incubated with ab307692 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

This data was developed using ab307692, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Claudin 1 with ab307692 at 1/100 followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Membranous staining on mouse colon (PMID : 24626855). The section was incubated with ab307692 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

This data was developed using ab307692, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Claudin 1 with ab307692 at 1/100 followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Membranous staining on rat colon. The section was incubated with ab307692 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Lab

Immunoprecipitation - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

This data was developed using ab307692, the same antibody clone in a different buffer formulation.

Claudin 1 was immunoprecipitated from 0.35 mg ab307692 IP in Mouse liver tissue lysate with ab307692 at 30. Western blot was performed on the immunoprecipitate using ab307692 at 1000 dilution.

All lanes:

Immunoprecipitation - Anti-Claudin 1 antibody [EPR25359-48] (<a href='/en-us/products/primary-antibodies/claudin-1-antibody-epr25359-48-ab307692'>ab307692</a>) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/claudin-1-antibody-epr25359-48-ab307692'>ab307692</a> at 1/30 IP in Mouse liver tissue lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/claudin-1-antibody-epr25359-48-ab307692'>ab307692</a> in mouse liver whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 19 kDa

false

Exposure time: 41s

Lab

Western blot - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

This data was developed using ab307692, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Claudin 1 antibody [EPR25359-48] (<a href='/en-us/products/primary-antibodies/claudin-1-antibody-epr25359-48-ab307692'>ab307692</a>) at 1/1000 dilution

Lane 1:

A431 whole cell lysate at 20 µg

Lane 2:

CLDN1 knockout A431 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 19 kDa

false

Exposure time: 3s

Lab

Western blot - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

This data was developed using ab307692, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

ab307692 may serve as a viable alternative to ab180158 in Western blot.

Lanes 1 - 6:

Western blot - Anti-Claudin 1 antibody [EPR25359-48] (<a href='/en-us/products/primary-antibodies/claudin-1-antibody-epr25359-48-ab307692'>ab307692</a>) at 1/1000 dilution

Lanes 7 - 12:

Western blot - Anti-Claudin 1 antibody [EPR9306] (<a href='/en-us/products/primary-antibodies/claudin-1-antibody-epr9306-ab180158'>ab180158</a>) at 1/1000 dilution

Lanes 1 and 7:

Mouse kidney at 10 µg

Lanes 2 and 8:

Mouse liver at 10 µg

Lanes 3 and 9:

Mouse colon at 10 µg

Lanes 4 and 10:

Rat kidney at 10 µg

Lanes 5 and 11:

Rat liver at 10 µg

Lanes 6 and 12:

Rat colon at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 19 kDa

false

Exposure time: 60s

Unconjugated

Anti-Claudin 1 antibody [EPR25359-48]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Claudin 1 antibody [EPR25359-48]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Claudin 1 antibody [EPR25359-48]
Unconjugated

Anti-Claudin 1 antibody [RM1186]
Carrier free

Anti-Claudin 1 antibody [RM1186] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25359-48

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin 1 also known as CLDN1 is a protein that plays a fundamental role in the formation of tight junctions in the epithelial cells. This claudin protein has a molecular weight of approximately 22 kDa. You can find it expressed in various tissues including the skin liver kidney and lungs. Claudin 1 belongs to the claudin family which consists of transmembrane proteins involved in sealing the space between adjacent cells therefore ensuring tissue integrity.

Biological function summary

This claudin protein contributes to establishing and maintaining the barrier function that controls paracellular transport which is the movement of substances between cells. Claudin 1 participates as a critical component of the tight junction complex and it interacts with other proteins such as occludin and zonula occludens. These interactions contribute to the regulation and maintenance of cell polarity and signaling.

Pathways

Claudin 1 is involved in the epithelial cell signaling pathways that influence cellular proliferation and differentiation. One significant pathway is the Wnt signaling pathway where claudin 1 cooperates with other claudins and proteins like β-catenin to regulate gene transcription. Additionally it is implicated in the MAPK pathway which involves signaling cascades that affect cell growth division and response to external stress.

Claudin 1 shows a strong association with cancers particularly hepatocellular carcinoma and melanoma. In these conditions changes in claudin 1 expression levels can affect tumor progression and metastasis. It also associates with other proteins like E-cadherin impacting cell adhesion and epithelial-mesenchymal transition in cancerous cells. Moreover abnormalities in claudin 1 expression relate to skin disorders such as ichthyosis which is marked by a dysfunction in the skin barrier.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions.

See full target information Cldn1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

Immunoprecipitation - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

Western blot - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

Western blot - Anti-Claudin 1 antibody [EPR25359-48] - BSA and Azide free (AB307693)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise