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AB52234

Anti-Claudin 10 antibody

5

(3 Reviews)

|

(6 Publications)

Rabbit Polyclonal Claudin 10 antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human Claudin-10.

View Alternative Names

Claudin-10, Oligodendrocyte-specific protein-like, OSP-like, CLDN10

1 Images
Western blot - Anti-Claudin 10 antibody (AB52234)
  • WB

Unknown

Western blot - Anti-Claudin 10 antibody (AB52234)

All lanes:

Western blot - Anti-Claudin 10 antibody (ab52234) at 1/300 dilution

Lane 1:

Extracts from LOVO cells, untreated.

Lane 2:

Extracts from LOVO cells, treated with the immunising peptide.

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ELISA, IHC-P

applications

Immunogen

Synthetic Peptide within Human Claudin-10. The exact immunogen used to generate this antibody is proprietary information.

P78369

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as Claudin 10 also referred to as CLDN10 is a member of the claudin family essential for formation and maintenance of tight junctions. Claudin 10 has a molecular weight of approximately 24 kDa. This protein is expressed widely across various tissues with significant levels in the kidney liver and pancreas. Its main role involves controlling the permeability of epithelial barriers contributing to paracellular transport regulation.
Biological function summary

The function of Claudin 10 includes its involvement in forming and maintaining tight junction strands within epithelial and endothelial cells. It participates in a complex with other claudins to create selectively permeable barriers that regulate ion transport. Claudin 10 plays a role in determining cation selectivity and permeability impacting various physiological processes such as electrolyte balance and fluid homeostasis. In kidney tubules it especially influences the reabsorption and secretion of ions.

Pathways

Claudin 10 interacts with signaling mechanisms that regulate epithelial tight junction integrity. One significant pathway is the Wnt signaling pathway which influences cell polarity and growth affecting tight junction dynamics. Claudin 10 also associates with proteins related to the Par3/Par6/aPKC complex within these pathways. Its function intertwines with those of other tight junction proteins such as ZO-1 and occludin forming a network vital for epithelial cell barrier function and tissue homeostasis.

Altered expression of Claudin 10 has links to kidney disorders such as hypermagnesemia with hypocalciuria due to its important role in magnesium reabsorption. Additionally changes in Claudin 10 function associate with certain liver conditions where disrupted tight junctions may lead to compromised epithelial barrier function. This protein's interaction through disorders often involves connections with the chloride channel protein CLCNKB highlighting its role in ion balance and transport.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Forms paracellular channels : polymerizes in tight junction strands with cation- and anion-selective channels through the strands, conveying epithelial permeability in a process known as paracellular tight junction permeability.. Isoform 1. Forms cation-selective paracellular channels. In sweat glands and in the thick ascending limb (TAL) of Henle's loop in kidney, it controls paracellular sodium permeability which is essential for proper sweat production and renal function (PubMed : 19383724, PubMed : 28686597, PubMed : 28771254, PubMed : 35650657, PubMed : 36008380).. Isoform 2. Forms anion-selective paracellular channels. In renal proximal tubules, it conveys selective chloride over hydrogencarbonate anion permeability which is required for renal chloride reabsorption and salt homeostasis.
See full target information Claudin-10
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 18:2329-2344 PubMed35414767

2022

Claudin-10 overexpression suppresses human clear cell renal cell carcinoma growth and metastasis by regulating ATP5O and causing mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Wuping Yang,Kenan Zhang,Zedan Zhang,Jingcheng Zhou,Lei Li,Yawei Xu,Jianhui Qiu,Lin Cai,Yanqing Gong,Kan Gong

Cellular microbiology 23:e13343 PubMed33864347

2021

Disassembly of the apical junctional complex during the transmigration of Leptospira interrogans across polarized renal proximal tubule epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Sebastián,Nobuhiko Okura,Bruno M Humbel,Jun Xu,Idam Hermawan,Chiaki Matsuura,Malgorzata Hall,Chitoshi Takayama,Tetsu Yamashiro,Shuichi Nakamura,Claudia Toma

Scientific reports 10:6257 PubMed32277131

2020

Intermittent exposure to whole cigarette smoke alters the differentiation of primary small airway epithelial cells in the air-liquid interface culture.

Applications

Unspecified application

Species

Unspecified reactive species

Julia A Gindele,Tobias Kiechle,Kerstin Benediktus,Gerald Birk,Michael Brendel,Fabian Heinemann,Christian T Wohnhaas,Michelle LeBlanc,Haijun Zhang,Yael Strulovici-Barel,Ronald G Crystal,Matthew J Thomas,Birgit Stierstorfer,Karsten Quast,Jürgen Schymeinsky

American journal of physiology. Renal physiology 318:F1138-F1146 PubMed32174144

2020

Combinatorial expression of claudins in the proximal renal tubule and its functional consequences.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua N Curry,Shinsaku Tokuda,Patrick McAnulty,Alan S L Yu

Molecular medicine reports 12:1753-60 PubMed25892273

2015

Effects of mesenchymal stem cells on interleukin-1β-treated chondrocytes and cartilage in a rat osteoarthritic model.

Applications

WB

Species

Unspecified reactive species

Jilei Tang,Weiding Cui,Fanglong Song,Chenjun Zhai,Hansheng Hu,Qiang Zuo,Weimin Fan

Molecular and cellular biology 33:2535-50 PubMed23608536

2013

Delineation of the key aspects in the regulation of epithelial monolayer formation.

Applications

Unspecified application

Species

Unspecified reactive species

Lydia Aschauer,Leonhard N Gruber,Walter Pfaller,Alice Limonciel,Toby J Athersuch,Rachel Cavill,Abdulhameed Khan,Gerhard Gstraunthaler,Johannes Grillari,Regina Grillari,Philip Hewitt,Martin O Leonard,Anja Wilmes,Paul Jennings
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB24792

Anti-Claudin 10 antibody

primary-antibodies

claudin-10-antibody-ab24792

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(0 reviews)

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