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AB19035

Anti-Claudin 14 antibody

4

(2 Reviews)

|

(3 Publications)

Goat Polyclonal Claudin 14 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CLDN14 aa 200 to C-terminus.

View Alternative Names

UNQ777/PRO1571, CLDN14, Claudin-14

1 Images
Western blot - Anti-Claudin 14 antibody (AB19035)
  • WB

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Western blot - Anti-Claudin 14 antibody (AB19035)

ab19035 (1μg/ml) staining of Human Liver lysate (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ab19035 (0.3 µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-Claudin 14 antibody (ab19035)

Predicted band size: 26 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CLDN14 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95500

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin-14 (CLDN14) plays an important role in the mechanics of epithelial tight junctions contributing to paracellular transport regulation. It forms a barrier that controls the flow of ions and small molecules between cells. CLDN14 has a molecular weight of around 23 kDa. It expresses mainly in the epithelial cells of various tissues including the kidney where it regulates calcium reabsorption and balance in the body.
Biological function summary

Claudin-14 functions as an integral component of tight junction complexes. It interacts with other claudins occludins and junctional adhesion molecules to maintain the paracellular permeability. This protein contributes to the formation and function of the tight junctional structures ultimately influencing ion selectivity and permeability. Studies have shown its role in maintaining electrolyte composition and osmotic balance in cellular environments.

Pathways

Claudin-14 integrates in the calcium-sensing pathway and the tight junction signaling pathway. It associates with calcium-sensing receptors (CaSR) in the kidneys impacting calcium homeostasis. Tight junction signaling involves interactions with proteins such as ZO-1 which is important for maintaining tight junction integrity and regulating epithelial barriers. These connections ensure the coordination of ion transport and stringent control of cellular environments.

Mutations or dysregulations in Claudin-14 link to familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC) and sensorineural deafness. FHHNC results from impaired calcium reabsorption affecting kidney function and calcium balance. Sensorineural deafness arises due to disorder in ion homeostasis in the inner ear where Claudin-14 is also present. Its interactions with CaSR and other tight junction proteins highlight the importance of Claudin-14 in maintaining specific physiological balances related to these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
See full target information CLDN14
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 60: PubMed34913069

2021

Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Amina Bolatkan,Ken Asada,Syuzo Kaneko,Kruthi Suvarna,Noriko Ikawa,Hidenori Machino,Masaaki Komatsu,Shuichiro Shiina,Ryuji Hamamoto

International journal of clinical and experimental pathology 12:2195-2204 PubMed31934042

2019

Identification of claudin-2, -6, -11 and -14 as prognostic markers in human breast carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyao Jia,Xin Chai,Sijie Li,Di Wu,Zhimin Fan

The Journal of biological chemistry 286:17766-76 PubMed21454667

2011

Identification of transcription factor E3 (TFE3) as a receptor-independent activator of Gα16: gene regulation by nuclear Gα subunit and its activator.

Applications

IHC-P

Species

Mouse

Motohiko Sato,Masahiro Hiraoka,Hiroko Suzuki,Yunzhe Bai,Reiko Kurotani,Utako Yokoyama,Satoshi Okumura,Mary J Cismowski,Stephen M Lanier,Yoshihiro Ishikawa
View all publications
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Product promise

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