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AB250897

Anti-Claudin 17 antibody [EPR15483] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Claudin 17 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

UNQ758/PRO1489, CLDN17, Claudin-17

3 Images
Western blot - Anti-Claudin 17 antibody [EPR15483] - BSA and Azide free (AB250897)
  • WB

Supplier Data

Western blot - Anti-Claudin 17 antibody [EPR15483] - BSA and Azide free (AB250897)

This data was developed using ab186744, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Claudin 17 antibody [EPR15483] (<a href='/en-us/products/primary-antibodies/claudin-17-antibody-epr15483-ab186744'>ab186744</a>) at 1/1000 dilution

All lanes:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Western blot - Anti-Claudin 17 antibody [EPR15483] - BSA and Azide free (AB250897)
  • WB

Supplier Data

Western blot - Anti-Claudin 17 antibody [EPR15483] - BSA and Azide free (AB250897)

This data was developed using ab186744, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Claudin 17 antibody [EPR15483] (<a href='/en-us/products/primary-antibodies/claudin-17-antibody-epr15483-ab186744'>ab186744</a>) at 1/10000 dilution

All lanes:

Human muscle lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa,45 kDa,54 kDa

Observed band size: 25 kDa,45 kDa

false

Western blot - Anti-Claudin 17 antibody [EPR15483] - BSA and Azide free (AB250897)
  • WB

Supplier Data

Western blot - Anti-Claudin 17 antibody [EPR15483] - BSA and Azide free (AB250897)

This data was developed using ab186744, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Claudin 17 antibody [EPR15483] (<a href='/en-us/products/primary-antibodies/claudin-17-antibody-epr15483-ab186744'>ab186744</a>) at 1/1000 dilution

Lane 1:

Rat kidney lysate at 10 µg

Lane 2:

Rat spleen lysate at 10 µg

Lane 3:

Raw 264.7 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15483

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Western blot - Anti-Claudin 1 antibody (AB15098)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250897 is the carrier-free version of ab186744.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin 17 also known as CLDN17 is a member of the claudin family of proteins essential for the formation of tight junctions in epithelial cells. Weighing approximately 24 kDa Claudin 17 plays a mechanical role in regulating paracellular transport which means it controls the flow of molecules between cells. Expression of this protein occurs mainly in the kidney and liver epithelia where it contributes to the barrier function of these tissues.
Biological function summary

Claudin 17 functions to maintain the selective permeability of epithelial barriers. It is part of the tight junction complex a group of proteins that includes other claudins occludin and junctional adhesion molecules. This complex ensures that tissues maintain homeostasis by controlling the diffusion of solutes and ions across epithelial layers. Claudin 17 also contributes to the polarity and differentiation of epithelial cells impacting how these cells interact with their environment.

Pathways

Researchers have identified Claudin 17 as an important player in the regulation of the paracellular transport pathway and its involvement in maintaining the epithelial barrier function. It participates in pathways that involve sodium and chloride ion transport closely interacting with proteins like Claudin 8 and Claudin 1. Such interactions balance ion transport and cellular permeability important for proper epithelial function.

Researchers have explored Claudin 17's involvement in conditions such as electrolyte imbalance and kidney dysfunction. Its altered expression has been connected to Claudin 3 in renal disorders impacting tight junction integrity. Misregulation could lead to disruptions in ion balance contributing to disease progression. Understanding how Claudin 17 relates to these conditions can drive advancements in therapeutic strategies against epithelial barrier-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Channel-forming tight junction protein with selectivity for anions, including chloride and hydrogencarbonate, and for solutes smaller than 9 Angstrom in diameter. In the kidney proximal tubule, may be involved in paracellular reabsorption of filtered anions. Does not affect water permeability.
See full target information CLDN17
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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