AB241330

Anti-Claudin 18.2 antibody [EPR19202-244]

BOND RX™ Validated
RabMAb
Recombinant
20ul selling size
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(2 Publications)

Anti-Claudin 18.2 antibody [EPR19202-244] (ab241330) is a rabbit monoclonal antibody detecting Claudin 18.2 in IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

UNQ778/PRO1572, CLDN18, Claudin-18

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemical analysis of formalin fixed paraffin embedded human stomach labelling Claudin 18.2 with ab241330 at a concentration of 2µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

ab241330 Anti-Claudin 18.2 antibody [EPR19202-244] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemical analysis of paraffin-embedded human normal pancreas (A) and pancreatic cancer (B) tissue labeling Claudin 18.2 with ab241330 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. No staining on human normal pancreas (negative control), but membranous staining on human pancreas cancer cells (PMID : 21832145) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemical analysis of Human gastric cancer with ab241330 at 1/600 dilution (0.46 μg/ml), followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use.Positive staining on human gastric cancer. The section was incubated with ab241330 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with hematoxylin.

Secondary antibody only control : Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemical analysis of paraffin-embedded human lung (A) and stomach (B) tissue labeling Claudin 18.2 with ab241330 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. No staining on human lung (negative control), but membranous staining on human gastric epithelial cells (PMID : 19047087) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemical analysis of paraffin-embedded mouse lung (A) and stomach (B) tissue labeling Claudin 18.2 with ab241330 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. No staining on mouse lung (negative control), but membranous staining on mouse gastric epithelial cells (PMID : 19047087) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Frozen sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat stomach tissue labeling Claudin 18.2 with ab241330 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Membranous staining on rat stomach is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemical analysis of paraffin-embedded rat lung (A) and stomach (B) tissue labeling Claudin 18.2 with ab241330 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. No staining on rat lung (negative control), but membranous staining on rat gastric epithelial cells (PMID : 19047087) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse lung tissue labeling Claudin 18.2 with ab241330 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Negative control : No staining on mouse lung (PMID : 19047087) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat lung tissue labeling Claudin 18.2 with ab241330 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Negative control : No staining on mouse lung (PMID : 19047087) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse stomach tissue labeling Claudin 18.2 with ab241330 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Membranous staining on mouse stomach is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Carrier free

Anti-Claudin 18.2 antibody [EPR19202-244] - BSA and Azide free
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Claudin 18.2 antibody [EPR19202-244]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Claudin18.2 antibody [EPR19202-244]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Claudin 18.2 antibody [EPR19202-244]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Claudin18.2 antibody [EPR19202-244]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19202-244

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/600", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/600", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/600", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

What is this antibody validated in?
Anti-Claudin 18.2 antibody [EPR19202-244] (ab241330) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR19202-244] also available for your convenience: ab241330, Carrier free - ab242079, Alexa Fluor® 594 - ab311780, Alexa Fluor® 568 - ab313061, Alexa Fluor® 555 - ab313261, Alexa Fluor® 750 - ab321490

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin 18.2 also known as claudin 18 CLDN18 or claudin-18.2 is a tight junction protein with a molecular mass of approximately 27 kDa. It is primarily expressed in gastric epithelial cells and certain lung tissues. Claudin 18.2 is part of the claudin family which plays a critical role in forming tight junctions to regulate paracellular transport and maintain cellular polarity. This isoform specifically expressed in the stomach is encoded by the CLDN18 gene located on chromosome 3. Researchers often use techniques like Immunohistochemistry (IHC) referred to as claudin 18.2 IHC or cldn18 IHC to study its expression and localization.

Biological function summary

Claudin 18.2 functions within tight junction complexes creating a barrier and controlling the movement of ions and molecules between cells. It is vital for the integrity and function of epithelial cell barriers in the stomach. The protein interacts with other claudins and cytoplasmic proteins to form these tight junctions essential for maintaining tissue organization and permeability. Its unique expression pattern makes it a significant marker for gastric tissue and a potential target for therapeutics.

Pathways

Claudin 18.2 is involved in cellular pathways related to adhesion and barrier function. It plays a role in the tight junction pathway critical for epithelial barrier integrity. This pathway involves interactions with other claudin proteins and ZO-1 which is a cytoplasmic plaque protein that links claudins to the actin cytoskeleton reinforcing cellular structure. Another pathway where claudin 18.2 is important is the gastro-intestinal epithelium maintenance pathway which is fundamental for protecting gastric tissues.

Claudin 18.2 is a notable target in cancers especially gastric cancer. Its specific expression in gastric tissues makes it an attractive target for cancer immunotherapy as aberrant expression could indicate gastric cancer onset. Another disorder related to claudin 18.2 is asthma particularly where lung epithelial cells express this protein. Studies have identified links between claudin 18.2 and increased permeability in lung tissues implicating it in asthmatic pathophysiology. Its association with these conditions highlights the potential for claudin 18.2 as a diagnostic or therapeutic target in oncology and respiratory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in alveolar fluid homeostasis via regulation of alveolar epithelial tight junction composition and therefore ion transport and solute permeability, potentially via downstream regulation of the actin cytoskeleton organization and beta-2-adrenergic signaling (By similarity). Required for lung alveolarization and maintenance of the paracellular alveolar epithelial barrier (By similarity). Acts to maintain epithelial progenitor cell proliferation and organ size, via regulation of YAP1 localization away from the nucleus and thereby restriction of YAP1 target gene transcription (By similarity). Acts as a negative regulator of RANKL-induced osteoclast differentiation, potentially via relocation of TJP2/ZO-2 away from the nucleus, subsequently involved in bone resorption in response to calcium deficiency (By similarity). Mediates the osteoprotective effects of estrogen, potentially via acting downstream of estrogen signaling independently of RANKL signaling pathways (By similarity).. Isoform A1. Involved in the maintenance of homeostasis of the alveolar microenvironment via regulation of pH and subsequent T-cell activation in the alveolar space, is therefore indirectly involved in limiting C.neoformans infection.. Isoform A2. Required for the formation of the gastric paracellular barrier via its role in tight junction formation, thereby involved in the response to gastric acidification.

See full target information CLDN18

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 57:260-276.e9 PubMed35077680

2022

Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rani Ojha,Ishaq Tantray,Suman Rimal,Siddhartha Mitra,Sam Cheshier,Bingwei Lu

Cell 184:6119-6137.e26 PubMed34890551

2021

Microenvironment drives cell state, plasticity, and drug response in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Srivatsan Raghavan,Peter S Winter,Andrew W Navia,Hannah L Williams,Alan DenAdel,Kristen E Lowder,Jennyfer Galvez-Reyes,Radha L Kalekar,Nolawit Mulugeta,Kevin S Kapner,Manisha S Raghavan,Ashir A Borah,Nuo Liu,Sara A Väyrynen,Andressa Dias Costa,Raymond W S Ng,Junning Wang,Emma K Hill,Dorisanne Y Ragon,Lauren K Brais,Alex M Jaeger,Liam F Spurr,Yvonne Y Li,Andrew D Cherniack,Matthew A Booker,Elizabeth F Cohen,Michael Y Tolstorukov,Isaac Wakiro,Asaf Rotem,Bruce E Johnson,James M McFarland,Ewa T Sicinska,Tyler E Jacks,Ryan J Sullivan,Geoffrey I Shapiro,Thomas E Clancy,Kimberly Perez,Douglas A Rubinson,Kimmie Ng,James M Cleary,Lorin Crawford,Scott R Manalis,Jonathan A Nowak,Brian M Wolpin,William C Hahn,Andrew J Aguirre,Alex K Shalek

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Claudin 18.2 antibody [EPR19202-244]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemistry (Frozen sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemistry (Frozen sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemistry (Frozen sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Immunohistochemistry (Frozen sections) - Anti-Claudin 18.2 antibody [EPR19202-244] (AB241330)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise