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Rabbit Recombinant Monoclonal Claudin 2 antibody. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-Claudin 2 antibody [EPR28730-220] (AB317317), expandable thumbnail
  • Western blot - Anti-Claudin 2 antibody [EPR28730-220] (AB317317), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Forms paracellular channels: polymerizes in tight junction strands with cation- and water-selective channels through the strands, conveying epithelial permeability in a process known as paracellular tight junction permeability (PubMed:20460438, PubMed:36008380). In intestinal epithelium, allows for sodium and water fluxes from the peritoneal side to the lumen of the intestine to regulate nutrient absorption and clear enteric pathogens as part of mucosal immune response (By similarity). In kidney, allows passive sodium and calcium reabsorption across proximal tubules from the lumen back to the bloodstream (By similarity). In the hepatobiliary tract, allows paracellular water and cation fluxes in the hepatic perivenous areas and biliary epithelium to generate bile flow and maintain osmotic gradients (By similarity).

Alternative names

PSEC0059, SP82, UNQ705/PRO1356, CLDN2, Claudin-2

Recommended products

Rabbit Recombinant Monoclonal Claudin 2 antibody. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28730-220
Purification technique
Affinity purification Protein A
Specificity

Not suitable for use with tissue lysates in WB. A non-specific band at ~25KDa is observed.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Claudin-2 also known as CLDN2 is a protein that plays an important role in the formation and function of tight junctions between epithelial and endothelial cells. This structural protein is approximately 22 kDa in mass. It is mainly expressed in tissues with high transepithelial transport such as the kidney and intestine. As a member of the claudins Claudin-2 contributes to the paracellular transport of small ions and water distinguishing itself from other tight junction proteins.

Biological function summary

Claudin-2 facilitates the creation of ion-selective pores in the tight junction complexes allowing it to regulate paracellular permeability and maintain ion balance. It is part of the larger tight junction complex that includes proteins like occludin and zona occludens. Through these interactions Claudin-2 supports the maintenance of epithelial barrier function which is essential for controlled passage of ions and molecules in tissues.

Pathways

Claudin-2 participates in the signaling pathways related to epithelial cell polarity and transepithelial transport. It is significantly involved in the MAPK and PI3K-Akt signaling pathways which are important for cell growth and survival. It includes interactions with related proteins such as Claudin-1 and Claudin-4 which modulate the structure and integrity of tight junctions in response to various cellular signals.

Associated diseases and disorders

An imbalance or dysregulation of Claudin-2 expression can contribute to pathological conditions like inflammatory bowel disease (IBD) and hypercalciuria. Defects in Claudin-2 disrupt barrier function increasing permeability and often correlating with disease progression. Additionally proteins like ZO-1 and ZO-2 are associated with Claudin-2 in these disorders as they together influence the dynamics and composition of tight junctions under pathophysiological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Claudin 2 antibody [EPR28730-220] (ab317317), expandable thumbnail

    Western blot - Anti-Claudin 2 antibody [EPR28730-220] (ab317317)

    Western blot: Anti-Cldn2 antibody [EPR28730-220] (ab317317) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab317317 was shown to bind specifically to Cldn2. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Claudin 2 antibody [EPR28730-220] (ab317317) at 1/1000 dilution

    Lane 1: HT-29 cell lysate at 20 µg

    Lane 2: Human Kidney cell lysate at 20 µg

    Lane 3: HCT 116 cell lysate at 20 µg

    Secondary

    Lanes 1 - 3: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

    Lanes 1 - 3: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 20-24 kDa

  • Western blot - Anti-Claudin 2 antibody [EPR28730-220] (ab317317), expandable thumbnail

    Western blot - Anti-Claudin 2 antibody [EPR28730-220] (ab317317)

    Low expression: SW620, SW480, HCT 116 (PMID: 21383692), PANC-1.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 21383692).

    The identity of the higher MW bands at approximately 60 kDa and 110 kDa are unknown.

    The identity of the lower MW band at approximately 10 kDa in lane 1 is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Claudin 2 antibody [EPR28730-220] (ab317317) at 1/1000 dilution

    Lane 1: LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 3: HCT-15 (human colon epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 4: SW620 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 5: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 6: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 7: A549 (human lu carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 8: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 20 kDa, 36 kDa

    Exposure time: 81s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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