Anti-Claudin 2 antibody [EPR28730-220] - BSA and Azide free

• WB

Lab

Western blot - Anti-Claudin 2 antibody [EPR28730-220] - BSA and Azide free (AB317318)

This data was developed using ab317317, the same antibody clone in a different buffer formulation.

Western blot : Anti-Cldn2 antibody [EPR28730-220] (ab317317) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab317317 was shown to bind specifically to Cldn2. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Claudin 2 antibody [EPR28730-220] (<a href='/en-us/products/primary-antibodies/claudin-2-antibody-epr28730-220-ab317317'>ab317317</a>) at 1/1000 dilution

Lane 1:

HT-29 cell lysate at 20 µg

Lane 2:

Human Kidney cell lysate at 20 µg

Lane 3:

HCT 116 cell lysate at 20 µg

Secondary

Lanes 1 - 3:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 3:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 20-24 kDa

false

• WB

Supplier Data

Western blot - Anti-Claudin 2 antibody [EPR28730-220] - BSA and Azide free (AB317318)

This data was developed using ab317317, the same antibody clone in a different buffer formulation.

Low expression : SW620, SW480, HCT 116 (PMID : 21383692), PANC-1.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 21383692).

The identity of the higher MW bands at approximately 60 kDa and 110 kDa are unknown.

The identity of the lower MW band at approximately 10 kDa in lane 1 is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Claudin 2 antibody [EPR28730-220] (<a href='/en-us/products/primary-antibodies/claudin-2-antibody-epr28730-220-ab317317'>ab317317</a>) at 1/1000 dilution

Lane 1:

LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 2:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 3:

HCT-15 (human colon epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 4:

SW620 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 5:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 6:

HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 7:

A549 (human lu carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 8:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa,36 kDa

false

Exposure time: 81s