Rabbit Recombinant Monoclonal Claudin 2 antibody. Carrier free. Suitable for WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
WB | IP | ICC/IF | Flow Cyt (Intra) | IHC-P | |
---|---|---|---|---|---|
Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Claudin-2, SP82, UNQ705/PRO1356, SP82, PSEC0059, CLDN2
Rabbit Recombinant Monoclonal Claudin 2 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Claudin-2, SP82, UNQ705/PRO1356, SP82, PSEC0059, CLDN2
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR28730-220
Affinity purification Protein A
Not suitable for use with tissue lysates in WB. A non-specific band at ~25KDa is observed.
Blue Ice
+4°C
ab317318 is the carrier-free version of Anti-Claudin 2 antibody [EPR28730-220] ab317317.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Claudin-2 also known as CLDN2 is a protein that plays an important role in the formation and function of tight junctions between epithelial and endothelial cells. This structural protein is approximately 22 kDa in mass. It is mainly expressed in tissues with high transepithelial transport such as the kidney and intestine. As a member of the claudins Claudin-2 contributes to the paracellular transport of small ions and water distinguishing itself from other tight junction proteins.
Claudin-2 facilitates the creation of ion-selective pores in the tight junction complexes allowing it to regulate paracellular permeability and maintain ion balance. It is part of the larger tight junction complex that includes proteins like occludin and zona occludens. Through these interactions Claudin-2 supports the maintenance of epithelial barrier function which is essential for controlled passage of ions and molecules in tissues.
Claudin-2 participates in the signaling pathways related to epithelial cell polarity and transepithelial transport. It is significantly involved in the MAPK and PI3K-Akt signaling pathways which are important for cell growth and survival. It includes interactions with related proteins such as Claudin-1 and Claudin-4 which modulate the structure and integrity of tight junctions in response to various cellular signals.
An imbalance or dysregulation of Claudin-2 expression can contribute to pathological conditions like inflammatory bowel disease (IBD) and hypercalciuria. Defects in Claudin-2 disrupt barrier function increasing permeability and often correlating with disease progression. Additionally proteins like ZO-1 and ZO-2 are associated with Claudin-2 in these disorders as they together influence the dynamics and composition of tight junctions under pathophysiological conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-Claudin 2 antibody [EPR28730-220] ab317317, the same antibody clone in a different buffer formulation.
Western blot: Anti-Cldn2 antibody [EPR28730-220] (Anti-Claudin 2 antibody [EPR28730-220] ab317317) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-Claudin 2 antibody [EPR28730-220] ab317317 was shown to bind specifically to Cldn2. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Claudin 2 antibody [EPR28730-220] (Anti-Claudin 2 antibody [EPR28730-220] ab317317) at 1/1000 dilution
Lane 1: HT-29 cell lysate at 20 µg
Lane 2: Human Kidney cell lysate at 20 µg
Lane 3: HCT 116 cell lysate at 20 µg
Lanes 1 - 3: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution
Lanes 1 - 3: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 20-24 kDa
This data was developed using Anti-Claudin 2 antibody [EPR28730-220] ab317317, the same antibody clone in a different buffer formulation.
Low expression: SW620, SW480, HCT 116 (PMID: 21383692), PANC-1.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 21383692).
The identity of the higher MW bands at approximately 60 kDa and 110 kDa are unknown.
The identity of the lower MW band at approximately 10 kDa in lane 1 is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Claudin 2 antibody [EPR28730-220] (Anti-Claudin 2 antibody [EPR28730-220] ab317317) at 1/1000 dilution
Lane 1: LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: HCT-15 (human colon epithelial cell) whole cell lysate at 20 µg
Lane 4: SW620 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 6: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 7: A549 (human lu carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 8: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 20 kDa, 36 kDa
Exposure time: 81s
Low expression: SW620, SW480, HCT 116 (PMID: 21383692), PANC-1.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 21383692).
The identity of the higher MW bands at approximately 60 kDa and 110 kDa are unknown.
The identity of the lower MW band at approximately 10 kDa in lane 1 is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
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