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Rabbit Recombinant Monoclonal Claudin 2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-Claudin 2 antibody [EPR28730-220] - BSA and Azide free (AB317318), expandable thumbnail
  • Western blot - Anti-Claudin 2 antibody [EPR28730-220] - BSA and Azide free (AB317318), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Alternative names

Claudin-2, SP82, UNQ705/PRO1356, SP82, PSEC0059, CLDN2

Recommended products

Rabbit Recombinant Monoclonal Claudin 2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

Alternative names

Claudin-2, SP82, UNQ705/PRO1356, SP82, PSEC0059, CLDN2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR28730-220

Purification technique

Affinity purification Protein A

Specificity

Not suitable for use with tissue lysates in WB. A non-specific band at ~25KDa is observed.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab317318 is the carrier-free version of Anti-Claudin 2 antibody [EPR28730-220] ab317317.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Claudin-2 also known as CLDN2 is a protein that plays an important role in the formation and function of tight junctions between epithelial and endothelial cells. This structural protein is approximately 22 kDa in mass. It is mainly expressed in tissues with high transepithelial transport such as the kidney and intestine. As a member of the claudins Claudin-2 contributes to the paracellular transport of small ions and water distinguishing itself from other tight junction proteins.

Biological function summary

Claudin-2 facilitates the creation of ion-selective pores in the tight junction complexes allowing it to regulate paracellular permeability and maintain ion balance. It is part of the larger tight junction complex that includes proteins like occludin and zona occludens. Through these interactions Claudin-2 supports the maintenance of epithelial barrier function which is essential for controlled passage of ions and molecules in tissues.

Pathways

Claudin-2 participates in the signaling pathways related to epithelial cell polarity and transepithelial transport. It is significantly involved in the MAPK and PI3K-Akt signaling pathways which are important for cell growth and survival. It includes interactions with related proteins such as Claudin-1 and Claudin-4 which modulate the structure and integrity of tight junctions in response to various cellular signals.

Associated diseases and disorders

An imbalance or dysregulation of Claudin-2 expression can contribute to pathological conditions like inflammatory bowel disease (IBD) and hypercalciuria. Defects in Claudin-2 disrupt barrier function increasing permeability and often correlating with disease progression. Additionally proteins like ZO-1 and ZO-2 are associated with Claudin-2 in these disorders as they together influence the dynamics and composition of tight junctions under pathophysiological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Claudin 2 antibody [EPR28730-220] - BSA and Azide free (ab317318), expandable thumbnail

    Western blot - Anti-Claudin 2 antibody [EPR28730-220] - BSA and Azide free (ab317318)

    This data was developed using Anti-Claudin 2 antibody [EPR28730-220] ab317317, the same antibody clone in a different buffer formulation.

    Western blot: Anti-Cldn2 antibody [EPR28730-220] (Anti-Claudin 2 antibody [EPR28730-220] ab317317) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-Claudin 2 antibody [EPR28730-220] ab317317 was shown to bind specifically to Cldn2. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Claudin 2 antibody [EPR28730-220] (Anti-Claudin 2 antibody [EPR28730-220] ab317317) at 1/1000 dilution

    Lane 1: HT-29 cell lysate at 20 µg

    Lane 2: Human Kidney cell lysate at 20 µg

    Lane 3: HCT 116 cell lysate at 20 µg

    Secondary

    Lanes 1 - 3: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

    Lanes 1 - 3: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 20-24 kDa

  • Western blot - Anti-Claudin 2 antibody [EPR28730-220] - BSA and Azide free (ab317318), expandable thumbnail

    Western blot - Anti-Claudin 2 antibody [EPR28730-220] - BSA and Azide free (ab317318)

    This data was developed using Anti-Claudin 2 antibody [EPR28730-220] ab317317, the same antibody clone in a different buffer formulation.

    Low expression: SW620, SW480, HCT 116 (PMID: 21383692), PANC-1.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 21383692).

    The identity of the higher MW bands at approximately 60 kDa and 110 kDa are unknown.

    The identity of the lower MW band at approximately 10 kDa in lane 1 is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Claudin 2 antibody [EPR28730-220] (Anti-Claudin 2 antibody [EPR28730-220] ab317317) at 1/1000 dilution

    Lane 1: LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HCT-15 (human colon epithelial cell) whole cell lysate at 20 µg

    Lane 4: SW620 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 7: A549 (human lu carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 8: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 20 kDa, 36 kDa

    Exposure time: 81s

    Low expression: SW620, SW480, HCT 116 (PMID: 21383692), PANC-1.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 21383692).

    The identity of the higher MW bands at approximately 60 kDa and 110 kDa are unknown.

    The identity of the lower MW band at approximately 10 kDa in lane 1 is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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