Name: Anti-Claudin 3 antibody [EPR19971]
SKU: ab214487
Rating: 4 (3 reviews)

Anti-Claudin 3 antibody [EPR19971] (ab214487) is a rabbit monoclonal antibody detecting Claudin 3 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

Related conjugates and formulations

ab222485
Carrier free
Anti-Claudin 3 antibody [EPR19971] - BSA and Azide free
ab306179
Conjugated
PE Anti-Claudin 3 antibody [EPR19971]
ab306180
Conjugated
APC Anti-Claudin 3 antibody [EPR19971]
ab310079
Conjugated
Alexa Fluor® 647 Anti-Claudin 3 antibody [EPR19971]
ab312485
Conjugated
Alexa Fluor® 568 Anti-Claudin 3 antibody [EPR19971]
ab312009
Conjugated
Alexa Fluor® 555 Anti-Claudin 3 antibody [EPR19971]
ab306181
Conjugated
HRP Anti-Claudin 3 antibody [EPR19971]
ab310479
Conjugated
Alexa Fluor® 594 Anti-Claudin 3 antibody [EPR19971]
ab309713
Conjugated
Alexa Fluor® 488 Anti-Claudin 3 antibody [EPR19971]
ab321378
Conjugated
Alexa Fluor® 750 Anti-Claudin 3 antibody [EPR19971]

Images

Western blot - Anti-Claudin 3 antibody [EPR19971] (AB214487), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

See full target information CLDN3

Function

Barrier-forming claudin. Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Alternative names

C7orf1, CPETR2, CLDN3, Claudin-3, Clostridium perfringens enterotoxin receptor 2, Rat ventral prostate.1 protein homolog, CPE-R 2, CPE-receptor 2, hRVP1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR19971
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Claudin 3 antibody [EPR19971] (ab214487) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of Claudin 3?

Anti-Claudin 3 [EPR19971] (ab214487) specifically detects a band for Claudin 3 (UniProt: O15551) at a molecular weight of 23kDa.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products

We have a range of other formats of antibody clone [EPR19971] also available for your convenience:
ab214487, Carrier free - ab222485, PE - ab306179, APC - ab306180, HRP - ab306181, Alkaline Phosphatase - ab308764, Alexa Fluor® 488 - ab309713, Alexa Fluor® 647 - ab310079, Alexa Fluor® 594 - ab310479, Alexa Fluor® 555 - ab312009, Alexa Fluor® 568 - ab312485, Alexa Fluor® 750 - ab321378

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein 'Claudin 3' also known simply as Claudin-3 or CLDN3 is an important component of tight junctions in epithelial and endothelial cells. Claudin 3 has a molecular weight of approximately 22 kDa. It is expressed in a variety of tissues including the liver lungs and kidneys. By forming channels within tight junctions Claudin 3 regulates paracellular transport and maintains the barrier properties of cell layers thereby playing a significant role in preserving cellular environments.

Biological function summary

Claudin 3 contributes to the establishment and function of tight junctions that create a selective barrier in epithelial cell layers. This protein works as part of a larger complex consisting of other claudin family members which together ensure the integrity and permeability of the barrier. Through these complexes Claudin 3 helps facilitate cell polarity and tissue homeostasis which are essential for normal cellular function and organization.

Pathways

Claudin 3 participates in signaling pathways such as the Wnt/beta-catenin pathway and the epidermal growth factor receptor (EGFR) pathway. Claudin 3 modulates these pathways alongside other claudin proteins and partners like zonula occludens proteins. These interactions are central to processes like tissue morphogenesis and wound healing where precise control of cellular communication and migration is required.

Associated diseases and disorders

Claudin 3 is linked to various cancers including ovarian and prostate cancer. Its expression is often altered in tumor cells where its dysregulation can lead to the disruption of tight junctions and contribute to cancer progression. In ovarian cancer Claudin 3 interacts with matrix metalloproteinases (MMPs) influencing the metastatic potential of cancer cells. Understanding Claudin 3's role can provide insights into new therapeutic targets for managing epithelial barrier dysfunction in cancer.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

Western blot - Anti-Claudin 3 antibody [EPR19971] (ab214487)
Blocking/Dilution buffer: 5% NFDM/TBST.
Doublet bands observed correspond to full length and processed forms of the claudin-3 protein. This is consistent with what has been described in the literature (PMID: 26170992).
All lanes: Western blot - Anti-Claudin 3 antibody [EPR19971] (ab214487) at 1/1000 dilution
Lane 1: SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg
Lane 2: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg
Lane 3: Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 20 kDa
Exposure time: 1s
Western blot - Anti-Claudin 3 antibody [EPR19971] (ab214487)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2: 1 minute; Lane 3: 3 seconds; Lane 4: 3 minutes.
Doublet bands observed correspond to full length and processed forms of the claudin-3 protein. This is consistent with what has been described in the literature (PMID: 26170992).
All lanes: Western blot - Anti-Claudin 3 antibody [EPR19971] (ab214487) at 1/1000 dilution
Lane 1: Human breast cancer lysate at 10 µg
Lane 2: Human pancreas lysate at 10 µg
Lane 3: Human ovary cancer lysate at 10 µg
Lane 4: Human fetal kidney lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/5000 dilution
Predicted band size: 23 kDa
Observed band size: 20 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 3 antibody [EPR19971] (ab214487)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Claudin 3 with ab214487 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Membrane staining on the pancreatic acinar cells of human pancreas is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 3 antibody [EPR19971] (ab214487)
Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling Claudin 3 with ab214487 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Membrane staining on the epithelium of human benign prostatic hyperplasia is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 3 antibody [EPR19971] (ab214487)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Claudin 3 with ab214487 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Membrane staining on the tumor cells of human breast cancer is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Claudin 3 antibody [EPR19971] (ab214487)
Claudin 3 was immunoprecipitated from 0.35 mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab214487 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214487 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution
Lane 1: MCF7 whole cell lysate 10μg (Input).
Lane 2: ab214487 IP in MCF7 whole cell lysate.
Lane 3:Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab214487 in MCF7 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
All lanes: Immunoprecipitation - Anti-Claudin 3 antibody [EPR19971] (ab214487)
Predicted band size: 23 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Claudin 3 antibody [EPR19971] (ab214487)
Immunofluorescent analysis of 100% methanol-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling Claudin 3 with ab214487 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membrane staining on MCF7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-Claudin 3 antibody [EPR19971] (ab214487)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling Claudin 3with ab214487 at 1/50 dilution (red) compared withRabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.