Rabbit Polyclonal Claudin 4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 34 publications.
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- Oncology letters 26:3352023Twist1‑mediated transcriptional activation of Claudin‑4 promotes cervical cancer cell migration and invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaqi Zhu,Qi JiangPubMed 37427351
- Antonie van Leeuwenhoek 116:565-5762023Tetrahydrocurcumin (THC) enhanced the clearance of Cryptococcus deneoformans during infection in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianli He,Zhiran Jin,Wei Hu,Xiaoxue Xia,Donghui Li,Weiyun Yao,Guangnan Li,Xuefeng Zhou,Guoqiang SongPubMed 37186068
- Physiological reports 10:e154292022Effects of different fluid management on lung and kidney during pressure-controlled and pressure-support ventilation in experimental acute lung injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEduardo Butturini de Carvalho,Ana Carolina Fernandes Fonseca,Raquel Ferreira Magalhães,Eliete Ferreira Pinto,Cynthia Dos Santos Samary,Mariana Alves Antunes,Camila Machado Baldavira,Lizandre Keren Ramos da Silveira,Walcy Rosolia Teodoro,Marcelo Gama de Abreu,Vera Luiza Capelozzi,Nathane Santanna Felix,Paolo Pelosi,Patrícia Rieken Macêdo Rocco,Pedro Leme SilvaPubMed 36065867
- Evidence-based complementary and alternative medicine : eCAM 2022:28181362022Baicalin Ameliorates DSS-Induced Colitis by Protecting Goblet Cells through Activating NLRP6 Inflammasomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanan Li,Jingyi Hu,Cheng Cheng,Feng Xu,Ryan Au,Lei Zhu,Hong ShenPubMed 36016685
- Cell cycle (Georgetown, Tex.) 21:1519-15312022Intestinal aging is alleviated by uridine via regulating inflammation and oxidative stress and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Jiang,Zhiwei ZhaoPubMed 35380925
- Frontiers in nutrition 8:8056122022Tea Tree Oil Terpinen-4-ol Protects Gut Barrier Integrity by Upregulation of Tight Junction Proteins the ERK1/2-Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanhong Yong,Biao Fang,Yingxin Huang,Junyu Li,Tianyue Yu,Lianyun Wu,Canying Hu,Xiaoxi Liu,Zhichao Yu,Xingbin Ma,Ravi Gooneratne,Sidong Li,A M Abd El-Aty,Xianghong JuPubMed 35155524
- Pancreas 50:1202-12112021Emodin Alleviates Intestinal Barrier Dysfunction by Inhibiting Apoptosis and Regulating the Immune Response in Severe Acute Pancreatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Zhou,Hong Xiang,Han Liu,Bing Qi,Xueying Shi,Wenhui Guo,Jiacheng Zou,Xueting Wan,Wenjing Wu,Zhengpeng Wang,Wenhui Liu,Shilin Xia,Dong ShangPubMed 34714285
- Clinical & translational immunology 10:e13002021IL-33/ST2 axis deficiency exacerbates neutrophil-dominant allergic airway inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiyun Ma,Yan Qian,Jingxian Jiang,Jingjing Wu,Meijuan Song,Xinyu Li,Zhongqi Chen,Zhengxia Wang,Ranran Zhu,Zhixiao Sun,Mao Huang,Ningfei Ji,Mingshun ZhangPubMed 34178329
- American journal of reproductive immunology (New York, N.Y. : 1989) 86:e134712021Alpha-linolenic acid regulates the gut microbiota and the inflammatory environment in a mouse model of endometriosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhexin Ni,Jie Ding,Qianqian Zhao,Wen Cheng,Jin Yu,Ling Zhou,Shuai Sun,Chaoqin YuPubMed 34022075
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 133:1110262021Alveolar epithelial glycocalyx shedding aggravates the epithelial barrier and disrupts epithelial tight junctions in acute respiratory distress syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Li,Zhijiang Qi,Dongxiao Li,Xiao Huang,Boyang Qi,Jiali Feng,Jianyu Qu,Xiaozhi WangPubMed 33378942
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Pig | Dilution info - | Notes - |
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Target data
Function
Can associate with other claudins to regulate tight junction structural and functional strand dynamics (PubMed:35773259, PubMed:36008380). May coassemble with CLDN8 into tight junction strands containing anion-selective channels that convey paracellular chloride permeability in renal collecting ducts (By similarity) (PubMed:36008380). May integrate into CLDN3 strands to modulate localized tight junction barrier properties (PubMed:35773259, PubMed:36008380). May disrupt strand assembly of channel-forming CLDN2 and CLDN15 and inhibit cation conductance (PubMed:35773259, PubMed:36008380). Cannot form tight junction strands on its own (PubMed:35773259, PubMed:36008380).
Alternative names
CPER, CPETR1, WBSCR8, CLDN4, Claudin-4, Clostridium perfringens enterotoxin receptor, Williams-Beuren syndrome chromosomal region 8 protein, CPE-R, CPE-receptor
Recommended products
Rabbit Polyclonal Claudin 4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 34 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Claudin-4 also known as CLDN4 is a protein essential for tight junctions which are critical in maintaining the permeability barrier of epithelial cells. The protein has a molecular mass of approximately 22 kDa. Claudin-4 expresses notably in epithelial cells of various tissues including the kidney lung and gastrointestinal tract. In addition to its presence in normal tissues claudin-4 can also exhibit heightened expression in certain cancerous tissues making it a subject of interest in oncology research.
- Biological function summary
Claudin-4 plays a significant role in paracellular transport and cell polarity. By assembling into complexes with other claudins and tight junction proteins claudin-4 regulates the selective permeability and homeostasis across cell layers. It functions as a component of tight junction strands contributing to their barrier properties. Interaction with proteins such as occludin and ZO-1 further defines its role in tight junction structure and function.
- Pathways
Claudin-4 participates in critical biological processes such as epithelial cell signaling and apoptosis regulation. It interfaces with the epithelial-to-mesenchymal transition (EMT) pathway where claudin-4 expression influences cellular adhesion and motility. Claudin-4 also interacts with related claudin proteins in these pathways contributing to structural arrangement and pathway signaling precision.
- Associated diseases and disorders
Claudin-4 has associations with several types of cancers including pancreatic and ovarian cancer. Its consistent overexpression in these conditions suggests its involvement in tumorigenesis and metastasis. In the context of ovarian cancer claudin-4 may interact with proteins such as mesothelin which plays a role in cell adhesion and tumor progression. Investigating claudin-4's involvement in cancer can provide insights into potential therapeutic targets and diagnostic markers.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Lean QY et al. PLoS One (2015) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody (ab15104)
Immunohistochemical analysis of healthy and colitis mouse colon sections (untreated and treated with enoxaparin) labeling claudin-4 with ab15104 at 1/200. Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab7090, anti-rabbit immunoglobulin G conjugated to horseradish peroxidase (HRP) at 1/300 was used as the secondary antibody. DAB Substrate Kit ab64238 at 1/50 was used to develop the histological signal.
Antigen retrieval was performed by incubating the sections for 10 minutes at 97°C in 1 mM EDTA buffer, pH 8.0 or 10 mM citrate buffer, pH 6.0.
Control, C; untreated colitis, DSS; oral enoxaparin, OE; intraperitoneal injection of enoxaparin, IPE.
Western blot - Anti-Claudin 4 antibody (ab15104)
All lanes: Western blot - Anti-Claudin 4 antibody (ab15104) at 1 µg/mL
All lanes: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 125 kDa, 20 kDa, 34 kDa, 35 kDa
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody (ab15104)
Formalin fixed paraffin embedded human tonsil stained with ab15104 (1/200, 10 min at room temperature).
Western blot - Anti-Claudin 4 antibody (ab15104)
False colour image of Western blot: Anti-Claudin 4 antibody staining at 1 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab15104 was shown to bind specifically to Claudin 4. A band was observed at 17 kDa in wild-type MCF7 cell lysates with no signal observed at this size in CLDN4 knockout cell line ab274946 (knockout cell lysate ab275004). To generate this image, wild-type and CLDN4 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Claudin 4 antibody (ab15104) at 1 µg/mL
Lane 1: Wild-type MCF7 cell lysate at 20 µg
Lane 2: Western blot - Human CLDN4 knockout MCF7 cell line (ab274946) at 20 µg
Lane 3: PC-3 cell lysate at 20 µg
Lane 4: U-2 OS cell lysate at 20 µg
Lane 5: LNCaP cell lysate at 20 µg
Lane 6: HeLa cell lysate at 20 µg
Lane 6: SW480 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 22 kDa
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