Rabbit Polyclonal Claudin 4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human CLDN4 aa 150 to C-terminus.
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- Frontiers in immunology 13:10461212022Immune and non-immune cell subtypes identify novel targets for prognostic and therapeutic strategy: A study based on intratumoral heterogenicity analysis of multicenter scRNA-seq datasets in lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianyu Fan,Jian Lu,Delei Niu,Yue Zhang,Bin Wang,Bei Zhang,Zugui Zhang,Xinjiai He,Nan Peng,Biao Li,Huilong Fang,Zheng Gong,Li ZhangPubMed 36483553
- Disease models & mechanisms 15:2022Short-term and bystander effects of radiation on murine submandibular glands.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHitoshi Uchida,Matthew H Ingalls,Eri O Maruyama,Carl J Johnston,Eric Hernady,Roberta C Faustoferri,Catherine E OvittPubMed 36263624
- Chemical research in toxicology 35:731-7492022Mycotoxins Activate the Aryl Hydrocarbon Receptor and Nrf2-ARE Pathway to Alter the Structure and Immune Response of Colon Epithelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Groestlinger,Veronika Spindler,Gudrun Pahlke,Michael Rychlik,Giorgia Del Favero,Doris MarkoPubMed 35405071
- Journal of neuroinflammation 19:762022Gut microbiota is causally associated with poststroke cognitive impairment through lipopolysaccharide and butyrate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuidi Wang,Mingsi Zhang,Jie Li,Jianhai Liang,Mengjia Yang,Genghong Xia,Yueran Ren,Hongwei Zhou,Qiheng Wu,Yan He,Jia YinPubMed 35379265
- Microscopy (Oxford, England) 71:152-1602022Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShoko Ishikawa,Misaki Nikaido,Takahito Otani,Kayoko Ogata,Hiroshi Iida,Yuko Inai,Sachio Tamaoki,Tetsuichiro InaiPubMed 35289919
- Viruses 14:2022Highly Pathogenic PRRSV-Infected Alveolar Macrophages Impair the Function of Pulmonary Microvascular Endothelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeifeng Sun,Weixin Wu,Nan Jiang,Xinna Ge,Yongning Zhang,Jun Han,Xin Guo,Lei Zhou,Hanchun YangPubMed 35336858
- Pflugers Archiv : European journal of physiology 474:315-3422022Effects of butyrate on ruminal Ca transport: evidence for the involvement of apically expressed TRPV3 and TRPV4 channels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFranziska Liebe,Hendrik Liebe,Gerhard Sponder,Stefan Mergler,Friederike StumpffPubMed 35098357
- Frontiers in pharmacology 12:6826142021Claudins: Beyond Tight Junctions in Human IBD and Murine Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSnježana Čužić,Maja Antolić,Anja Ognjenović,Darija Stupin-Polančec,Adriana Petrinić Grba,Boška Hrvačić,Miroslava Dominis Kramarić,Sanja Musladin,Lidija Požgaj,Ivo Zlatar,Denis Polančec,Gorana Aralica,Marko Banić,Marija Urek,Brankica Mijandrušić Sinčić,Aleksandar Čubranić,Ines Glojnarić,Martina Bosnar,Vesna Eraković HaberPubMed 34867313
- International journal of molecular sciences 22:2021Effects of 1,2-Dimethylhydrazine on Barrier Properties of Rat Large Intestine and IPEC-J2 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesViktoria Bekusova,Linda Droessler,Salah Amasheh,Alexander G MarkovPubMed 34638619
- Gut microbes 13:19463682021Chronic opioid use modulates human enteric microbiota and intestinal barrier integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAngélica Cruz-Lebrón,Ramona Johnson,Claire Mazahery,Zach Troyer,Samira Joussef-Piña,Miguel E Quiñones-Mateu,Christopher M Strauch,Stanley L Hazen,Alan D LevinePubMed 34313547
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CLDN4 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O14493
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Can associate with other claudins to regulate tight junction structural and functional strand dynamics (PubMed:35773259, PubMed:36008380). May coassemble with CLDN8 into tight junction strands containing anion-selective channels that convey paracellular chloride permeability in renal collecting ducts (By similarity) (PubMed:36008380). May integrate into CLDN3 strands to modulate localized tight junction barrier properties (PubMed:35773259, PubMed:36008380). May disrupt strand assembly of channel-forming CLDN2 and CLDN15 and inhibit cation conductance (PubMed:35773259, PubMed:36008380). Cannot form tight junction strands on its own (PubMed:35773259, PubMed:36008380).
Alternative names
CPER, CPETR1, WBSCR8, CLDN4, Claudin-4, Clostridium perfringens enterotoxin receptor, Williams-Beuren syndrome chromosomal region 8 protein, CPE-R, CPE-receptor
Recommended products
Rabbit Polyclonal Claudin 4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human CLDN4 aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CLDN4 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O14493
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Claudin-4 also known as CLDN4 is a protein essential for tight junctions which are critical in maintaining the permeability barrier of epithelial cells. The protein has a molecular mass of approximately 22 kDa. Claudin-4 expresses notably in epithelial cells of various tissues including the kidney lung and gastrointestinal tract. In addition to its presence in normal tissues claudin-4 can also exhibit heightened expression in certain cancerous tissues making it a subject of interest in oncology research.
- Biological function summary
Claudin-4 plays a significant role in paracellular transport and cell polarity. By assembling into complexes with other claudins and tight junction proteins claudin-4 regulates the selective permeability and homeostasis across cell layers. It functions as a component of tight junction strands contributing to their barrier properties. Interaction with proteins such as occludin and ZO-1 further defines its role in tight junction structure and function.
- Pathways
Claudin-4 participates in critical biological processes such as epithelial cell signaling and apoptosis regulation. It interfaces with the epithelial-to-mesenchymal transition (EMT) pathway where claudin-4 expression influences cellular adhesion and motility. Claudin-4 also interacts with related claudin proteins in these pathways contributing to structural arrangement and pathway signaling precision.
- Associated diseases and disorders
Claudin-4 has associations with several types of cancers including pancreatic and ovarian cancer. Its consistent overexpression in these conditions suggests its involvement in tumorigenesis and metastasis. In the context of ovarian cancer claudin-4 may interact with proteins such as mesothelin which plays a role in cell adhesion and tumor progression. Investigating claudin-4's involvement in cancer can provide insights into potential therapeutic targets and diagnostic markers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Claudin 4 antibody (ab53156)
All lanes: Western blot - Anti-Claudin 4 antibody (ab53156) at 1/500 dilution
Lane 1: Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts with immunizing peptide
Predicted band size: 22 kDa
Observed band size: 22 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Claudin 4 antibody (ab53156)
ICC/IF image of ab53156 staining Claudin 4 (green) in MCF7 (Human breast adenocarcinoma cell line) cells. The cells were fixed in 4% formaldehyde (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53156, 5 μg/ml) overnight at +4°C. The secondary antibody was an Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. An Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 μM.
Western blot - Anti-Claudin 4 antibody (ab53156)
False colour image of Western blot: Anti-Claudin 4 antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab53156 was shown to bind specifically to Claudin 4. A band was observed at 17 kDa in wild-type MCF7 cell lysates with no signal observed at this size in CLDN4 knockout cell line ab274946 (knockout cell lysate ab275004). To generate this image, wild-type and CLDN4 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Claudin 4 antibody (ab53156) at 1/500 dilution
Lane 1: Wild-type MCF7 cell lysate at 20 µg
Lane 2: Western blot - Human CLDN4 knockout MCF7 cell lysate (ab275004) at 20 µg
Lane 3: PC-3 cell lysate at 20 µg
Lane 4: U-2 OS cell lysate at 20 µg
Lane 5: LNCaP cell lysate at 20 µg
Lane 6: SW480 cell lysate at 20 µg
Lane 7: HeLa cell lysate at 20 µg
SecondaryAll lanes: Western blot at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 17 kDa
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