Anti-Claudin 4 antibody [EPRR17575] - BSA and Azide free (ab240384)

Knockout Tested Rabbit Recombinant Monoclonal Claudin 4 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody [EPRR17575] - BSA and Azide free (AB240384), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P
Human	Tested	Tested	Tested
Mouse	Expected	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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1 product for Alternative Version

Target data

See full target information CLDN4

Function

Can associate with other claudins to regulate tight junction structural and functional strand dynamics (PubMed:35773259, PubMed:36008380). May coassemble with CLDN8 into tight junction strands containing anion-selective channels that convey paracellular chloride permeability in renal collecting ducts (By similarity) (PubMed:36008380). May integrate into CLDN3 strands to modulate localized tight junction barrier properties (PubMed:35773259, PubMed:36008380). May disrupt strand assembly of channel-forming CLDN2 and CLDN15 and inhibit cation conductance (PubMed:35773259, PubMed:36008380). Cannot form tight junction strands on its own (PubMed:35773259, PubMed:36008380).

Alternative names

CPER, CPETR1, WBSCR8, CLDN4, Claudin-4, Clostridium perfringens enterotoxin receptor, Williams-Beuren syndrome chromosomal region 8 protein, CPE-R, CPE-receptor

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal Claudin 4 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPRR17575
Purification technique: Affinity purification Protein A
Specificity: The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab240384 is the carrier-free version of Anti-Claudin 4 antibody [EPRR17575] ab210796.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Claudin-4 also known as CLDN4 is a protein essential for tight junctions which are critical in maintaining the permeability barrier of epithelial cells. The protein has a molecular mass of approximately 22 kDa. Claudin-4 expresses notably in epithelial cells of various tissues including the kidney lung and gastrointestinal tract. In addition to its presence in normal tissues claudin-4 can also exhibit heightened expression in certain cancerous tissues making it a subject of interest in oncology research.

Biological function summary

Claudin-4 plays a significant role in paracellular transport and cell polarity. By assembling into complexes with other claudins and tight junction proteins claudin-4 regulates the selective permeability and homeostasis across cell layers. It functions as a component of tight junction strands contributing to their barrier properties. Interaction with proteins such as occludin and ZO-1 further defines its role in tight junction structure and function.

Pathways

Claudin-4 participates in critical biological processes such as epithelial cell signaling and apoptosis regulation. It interfaces with the epithelial-to-mesenchymal transition (EMT) pathway where claudin-4 expression influences cellular adhesion and motility. Claudin-4 also interacts with related claudin proteins in these pathways contributing to structural arrangement and pathway signaling precision.

Associated diseases and disorders

Claudin-4 has associations with several types of cancers including pancreatic and ovarian cancer. Its consistent overexpression in these conditions suggests its involvement in tumorigenesis and metastasis. In the context of ovarian cancer claudin-4 may interact with proteins such as mesothelin which plays a role in cell adhesion and tumor progression. Investigating claudin-4's involvement in cancer can provide insights into potential therapeutic targets and diagnostic markers.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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9 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody [EPRR17575] - BSA and Azide free (ab240384)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Claudin 4 with Anti-Claudin 4 antibody [EPRR17575] ab210796 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Membrane and staining on epithelial cells of human colon is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Claudin 4 antibody [EPRR17575] ab210796).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody [EPRR17575] - BSA and Azide free (ab240384)
Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling Claudin 4 with Anti-Claudin 4 antibody [EPRR17575] ab210796 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Membrane staining on epithelial cells of human endometrium is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Claudin 4 antibody [EPRR17575] ab210796).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody [EPRR17575] - BSA and Azide free (ab240384)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Claudin 4 with Anti-Claudin 4 antibody [EPRR17575] ab210796 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Weak membrane staining on biliary epithelium of human liver is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Claudin 4 antibody [EPRR17575] ab210796).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody [EPRR17575] - BSA and Azide free (ab240384)
Immunohistochemical analysis of paraffin-embedded humancholangiocarcinoma tissue labeling Claudin 4 with Anti-Claudin 4 antibody [EPRR17575] ab210796 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Membrane staining on human cholangiocarcinoma is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Claudin 4 antibody [EPRR17575] ab210796).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Claudin 4 antibody [EPRR17575] - BSA and Azide free (ab240384)
Claudin 4 was immunoprecipitated from 1mg of SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate with Anti-Claudin 4 antibody [EPRR17575] ab210796 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using Anti-Claudin 4 antibody [EPRR17575] ab210796 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: SW480 whole cell lysate 10μg (Input).
Lane 2: Anti-Claudin 4 antibody [EPRR17575] ab210796 IP in SW480 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Claudin 4 antibody [EPRR17575] ab210796 in SW480 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Claudin 4 antibody [EPRR17575] ab210796).
All lanes: Immunoprecipitation - Anti-Claudin 4 antibody [EPRR17575] (Anti-Claudin 4 antibody [EPRR17575] ab210796)
Predicted band size: 22 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody [EPRR17575] - BSA and Azide free (ab240384)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Claudin 4 with Anti-Claudin 4 antibody [EPRR17575] ab210796 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative staining on human hepatocellular carcinoma.
Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Claudin 4 antibody [EPRR17575] ab210796).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody [EPRR17575] - BSA and Azide free (ab240384)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Claudin 4 with Anti-Claudin 4 antibody [EPRR17575] ab210796 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Membrane staining on epithelial cells of human breast cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Claudin 4 antibody [EPRR17575] ab210796).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Claudin 4 antibody [EPRR17575] - BSA and Azide free (ab240384)
False colour image of Western blot: Anti-Claudin 4 antibody [EPRR17575] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Claudin 4 antibody [EPRR17575] ab210796 was shown to bind specifically to Claudin 4. A band was observed at 17 kDa in wild-type MCF7 cell lysates with no signal observed at this size in CLDN4 knockout cell line ab274946 (knockout cell lysate ab275004). To generate this image, wild-type and CLDN4 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Claudin 4 antibody [EPRR17575] ab210796).
All lanes: Western blot - Anti-Claudin 4 antibody [EPRR17575] (Anti-Claudin 4 antibody [EPRR17575] ab210796) at 1/1000 dilution
Lane 1: Wild-type MCF7 cell lysate at 20 µg
Lane 2: Western blot - Human CLDN4 knockout MCF7 cell lysate (ab275004)
Lane 3: PC-3 cell lysate at 20 µg
Lane 4: U-2 OS cell lysate at 20 µg
Lane 5: SW480 cell lysate at 20 µg
Secondary
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 17 kDa
Western blot - Anti-Claudin 4 antibody [EPRR17575] - BSA and Azide free (ab240384)
Blocking and diluting buffer and concentration: 5% NFDM/TBST

The identity of the higher MW band at approximately 35 kDa is unknown.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Claudin 4 antibody [EPRR17575] ab210796).
All lanes: Western blot - Anti-Claudin 4 antibody [EPRR17575] (Anti-Claudin 4 antibody [EPRR17575] ab210796) at 1/1000 dilution
All lanes: 4T1 (mouse mammary gland carcinoma epithelial cell), whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 22 kDa
Observed band size: 18 kDa
Exposure time: 3min