Rabbit Polyclonal Claudin 5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 133 publications.
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Publications
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- Journal of inflammation research 16:5095-51092023LPA Alleviates Septic Acute Lung Injury via Protective Endothelial Barrier Function Through Activation of PLC-PKC-FAK.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuifeng Bai,Jianqiu Pei,Shengqiang Pei,Xiangfeng Cong,Jerold Chun,Fang Wang,Xi ChenPubMed 38026263
- Fluids and barriers of the CNS 20:782023Human isogenic cells of the neurovascular unit exert transcriptomic cell type-specific effects on a blood-brain barrier in vitro model of late-onset Alzheimer disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUndine Haferkamp,Carla Hartmann,Chaudhry Luqman Abid,Andreas Brachner,Alevtina Höchner,Anna Gerhartl,Bernadette Harwardt,Selin Leckzik,Jennifer Leu,Marco Metzger,Marina Nastainczyk-Wulf,Winfried Neuhaus,Sabrina Oerter,Ole Pless,Dan Rujescu,Matthias Jung,Antje Appelt-MenzelPubMed 37907966
- Aging 15:10972-109952023Chronic kidney disease causes blood-brain barrier breakdown via urea-activated matrix metalloproteinase-2 and insolubility of tau protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHisazumi Matsuki,Shintaro Mandai,Hiroki Shiwaku,Takaaki Koide,Naohiro Takahashi,Tomoki Yanagi,Shunsuke Inaba,Saaya Ida,Tamami Fujiki,Yutaro Mori,Fumiaki Ando,Takayasu Mori,Koichiro Susa,Soichiro Iimori,Eisei Sohara,Hidehiko Takahashi,Shinichi UchidaPubMed 37889501
- Brain, behavior, & immunity - health 34:1006842023Universal method for the isolation of microvessels from frozen brain tissue: A proof-of-concept multiomic investigation of the neurovasculature.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarina Wakid,Daniel Almeida,Zahia Aouabed,Reza Rahimian,Maria Antonietta Davoli,Volodymyr Yerko,Elena Leonova-Erko,Vincent Richard,René Zahedi,Christoph Borchers,Gustavo Turecki,Naguib MechawarPubMed 37822873
- Cellular and molecular neurobiology 43:4173-41872023A Perfused In Vitro Human iPSC-Derived Blood-Brain Barrier Faithfully Mimics Transferrin Receptor-Mediated Transcytosis of Therapeutic Antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFloriana Burgio,Carine Gaiser,Kevin Brady,Viviana Gatta,Reiner Class,Ramona Schrage,Laura Suter-DickPubMed 37698826
- Current issues in molecular biology 45:3462-34782023Molecular Heterogeneity of the Brain Endothelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNada Alnaqbi,Mohammad G Mohammad,Rifat Hamoudi,Aloïse Mabondzo,Rania HaratiPubMed 37185751
- Neurotrauma reports 4:236-2502023Intranasal Leukemia Inhibitory Factor Attenuates Gliosis and Axonal Injury and Improves Sensorimotor Function After a Mild Pediatric Traumatic Brain Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVeera D'Mello,Malini Subramaniam,Aditya Paul Bhalla,Sherlyn Saavedra,Ofri Leiba,Steven W LevisonPubMed 37095853
- Biomolecules 13:2023Blood-Brain Barrier Integrity Is Perturbed in a -Null Mouse Model of Rett Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiuseppe Pepe,Salvatore Fioriniello,Federico Marracino,Luca Capocci,Vittorio Maglione,Maurizio D'Esposito,Alba Di Pardo,Floriana Della RagionePubMed 37189354
- International journal of molecular sciences 24:2023Loss of Blood-Brain Barrier Integrity in an In Vitro Model Subjected to Intermittent Hypoxia: Is Reversion Possible with a HIF-1α Pathway Inhibitor?Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Cloé Voirin,Morgane Chatard,Anne Briançon-Marjollet,Jean Louis Pepin,Nathalie Perek,Frederic RochePubMed 36902491
- International journal of molecular sciences 24:2023Genetic Interference of FGFR3 Impedes Invasion of Upper Tract Urothelial Carcinoma Cells by Alleviating RAS/MAPK Signal Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGong-Kai Huang,Chao-Cheng Huang,Chih-Hsiung Kang,Yuan-Tso Cheng,Po-Ching Tsai,Ying-Hsien Kao,Yueh-Hua ChungPubMed 36675289
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Not recommended |
| Mouse | Predicted | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Plays a major role in tight junction-specific obliteration of the intercellular space.
Alternative names
AWAL, TMVCF, TMVCF, AWAL, CLDN5, Claudin-5, Transmembrane protein deleted in VCFS, TMDVCF
Recommended products
Rabbit Polyclonal Claudin 5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 133 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Claudin 5 also known as CLDN5 is an integral component of tight junction strands within endothelial and epithelial cells. This protein possesses a molecular weight of approximately 23 kDa. It prominently expresses in the vascular endothelial cells of the blood-brain barrier and other blood-tissue barriers. Claudin 5 plays a mechanical role in forming selectively permeable paracellular barriers that regulate the movement of molecules and ions between cells. This function is essential in preserving the tissue-specific environment.
- Biological function summary
Claudin 5 serves as an important element in maintaining the integrity of the blood-brain barrier by creating a selective permeability. It operates as part of a larger tight junction complex interacting mainly with other claudin proteins and proteins such as occludin and ZO-1. These interactions ensure the structural and functional integrity of the cell junctions which helps stabilize the barrier properties against various physiological and pathological insults.
- Pathways
Claudin 5 is involved in important biological pathways such as the tight junction pathway and the barrier permeability regulation pathway. Through these pathways it interacts with proteins like occludin and various signaling molecules. This involvement allows tight regulation of barrier permeability ensuring selective transport and preventing the passage of harmful substances into sensitive areas like the central nervous system.
- Associated diseases and disorders
Claudin 5 plays a significant role in conditions like cerebral edema and Alzheimer's disease. Altered expression or dysfunction of Claudin 5 disrupts the blood-brain barrier contributing to these disorders. In Alzheimer's disease there is evidence of impaired tight junctions where Claudin 5 interacts with other claudins and related proteins like amyloid-beta affecting barrier integrity. Understanding these connections helps in developing therapeutic strategies targeting tight junction stability.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody (ab15106)
ab15106 staining Claudin 5 in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1.5% serum for 1 hour at 37°C; antigen retrieval was by heat mediation in a acetic acid. Samples were incubated with primary antibody (1/200 in blocking buffer) for 18 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody (ab15106)
ab15106 staining Claudin 5 in human lung by Immunohistochemistry (FFPE-sections).
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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