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AB131259

Anti-Claudin 5 antibody [EPR7583]

4

(7 Reviews)

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(135 Publications)

Anti-Claudin 5 antibody [EPR7583] (ab131259) is a rabbit monoclonal antibody detecting Claudin 5 in Western Blot, IHC-P, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 90 publications

View Alternative Names

AWAL, TMVCF, CLDN5, Claudin-5, Transmembrane protein deleted in VCFS, TMDVCF

13 Images
Western blot - Anti-Claudin 5 antibody [EPR7583] (AB131259)
  • WB

Unknown

Western blot - Anti-Claudin 5 antibody [EPR7583] (AB131259)

All lanes:

Western blot - Anti-Claudin 5 antibody [EPR7583] (ab131259) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human lung squamous cell cancer lysate at 10 µg

Lane 3:

Human small cell lung cancer lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 23 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-Claudin 5 antibody [EPR7583] (AB131259)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 5 antibody [EPR7583] (AB131259)

ab131259 staining Claudin 5 in HUV-EC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab131259 at 0.008µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)

Immunohistochemical analysis of formalin fixed paraffin embedded human lung labelling Claudin 5 with ab131259 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins. ab131259 Anti-Claudin 5 antibody [EPR7583] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)

Immunohistochemical analysis of formalin fixed paraffin embedded human spleen labelling Claudin 5 with ab131259 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins. ab131259 Anti-Claudin 5 antibody [EPR7583] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)

Unpurified ab131259 showing positive staining in Normal placenta tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)

Immunohistochemical staining of paraffin embedded human colon with purified ab131259 at a working dilution of 1/1000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Claudin 5 with unpurified ab131259 antibody at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)

Unpurified ab131259 showing positive staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)

Immunohistochemical analysis of paraffin embedded Human lung tissue labelling Claudin 5 with unpurified ab131259 antibody at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)

Unpurified ab131259 showing positive staining in Normal colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (AB131259)

Unpurified ab131259 showing positive staining in Normal uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Claudin 5 antibody [EPR7583] (AB131259)
  • WB

Lab

Western blot - Anti-Claudin 5 antibody [EPR7583] (AB131259)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Claudin 5 antibody [EPR7583] (ab131259) at 1/20000 dilution

All lanes:

Human lung squamous cell cancer lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/5000 dilution

Predicted band size: 11 kDa,118 kDa,13 kDa,23 kDa,25 kDa,27 kDa,29 kDa,33 kDa,34 kDa,35 kDa,66 kDa,69 kDa,70 kDa

Observed band size: 11 kDa,118 kDa,18 kDa,20 kDa,25-32 kDa,40 kDa,70 kDa,74 kDa

false

Western blot - Anti-Claudin 5 antibody [EPR7583] (AB131259)
  • WB

Lab

Western blot - Anti-Claudin 5 antibody [EPR7583] (AB131259)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Claudin 5 antibody [EPR7583] (ab131259) at 1/5000 dilution

All lanes:

Human small cell lung cancer at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/5000 dilution

Predicted band size: 23 kDa

Observed band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7583

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>ab131259 failed to detect target band in mouse brain tissue. This antibody is unsuitable to be used in IHC on mouse tissues.</p><p>This antibody failed to get signal in cell lines when performing western blot, so we do not recommend to use it when testing cell lines in westen blot.</p>

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AB15106

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Secondary Antibodies

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Reactivity data

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Product details

Product Specifications
Anti-Claudin 5 antibody [EPR7583] (ab131259) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, WB in human samples.
Anti-Claudin 5 antibody [EPR7583] (ab131259) specifically detects Claudin 5 (UniProt ID: O00501; Molecular weight: 23kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Claudin 5 antibody [EPR7583] (ab131259) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Claudin 5 antibody [EPR7583] (ab131259) has been cited over 90 times in peer reviewed journals and is trusted by the scientific community.
Anti-Claudin 5 antibody [EPR7583] (ab131259) has 7 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone EPR7583 - ab236066.
Antibody clone EPR7583 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 594, Alexa Fluor® 568, Alexa Fluor® 555, Alexa Fluor® 750 (ab311000, ab311126, ab311758, ab313038, ab313239, ab321040).

Target Information
Claudin-5 (CLDN5) is an integral membrane protein that forms tight junctions in endothelial cells, crucial for the blood-brain barrier's (BBB) function. It ensures the selective permeability of the BBB, preventing harmful substances from entering the brain. Abnormal expression of Claudin-5 is associated with several neurological conditions, such as epilepsy, Alzheimer's disease, and schizophrenia, highlighting its importance in maintaining BBB integrity and brain health.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin 5 also known as CLDN5 is an integral component of tight junction strands within endothelial and epithelial cells. This protein possesses a molecular weight of approximately 23 kDa. It prominently expresses in the vascular endothelial cells of the blood-brain barrier and other blood-tissue barriers. Claudin 5 plays a mechanical role in forming selectively permeable paracellular barriers that regulate the movement of molecules and ions between cells. This function is essential in preserving the tissue-specific environment.
Biological function summary

Claudin 5 serves as an important element in maintaining the integrity of the blood-brain barrier by creating a selective permeability. It operates as part of a larger tight junction complex interacting mainly with other claudin proteins and proteins such as occludin and ZO-1. These interactions ensure the structural and functional integrity of the cell junctions which helps stabilize the barrier properties against various physiological and pathological insults.

Pathways

Claudin 5 is involved in important biological pathways such as the tight junction pathway and the barrier permeability regulation pathway. Through these pathways it interacts with proteins like occludin and various signaling molecules. This involvement allows tight regulation of barrier permeability ensuring selective transport and preventing the passage of harmful substances into sensitive areas like the central nervous system.

Claudin 5 plays a significant role in conditions like cerebral edema and Alzheimer's disease. Altered expression or dysfunction of Claudin 5 disrupts the blood-brain barrier contributing to these disorders. In Alzheimer's disease there is evidence of impaired tight junctions where Claudin 5 interacts with other claudins and related proteins like amyloid-beta affecting barrier integrity. Understanding these connections helps in developing therapeutic strategies targeting tight junction stability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a major role in tight junction-specific obliteration of the intercellular space.
See full target information CLDN5
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Publications (135)

Recent publications for all applications. Explore the full list and refine your search

TH open : companion journal to thrombosis and haemostasis 9:a27014242 PubMed41059381

2025

Effects of DOACs on Mouse Melanoma Metastasis and the Inhibitory Mechanism of Edoxaban, a Factor Xa-Specific DOAC.

Applications

Unspecified application

Species

Unspecified reactive species

Keiichi Hiramoto,Taisei Watanabe,Masashi Imai,Koji Suzuki

Acta pharmaceutica Sinica. B 15:4248-4264 PubMed40893673

2025

Artificial mesenchymal stem cell extracellular vesicles enhanced ischemic stroke treatment through targeted remodeling brain microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shengnan Li,Wei Lv,Jiangna Xu,Jiaqing Yin,Yuqin Chen,Linfeng Liu,Xiang Cao,Wenjing Li,Zhen Li,Hua Chen,Hongliang Xin

Journal of translational medicine 23:958 PubMed40855559

2025

Quercetin promotes angiogenesis and protects the blood-spinal cord barrier structure after spinal cord injury by targeting the PI3K/Akt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinfang Liu,Xuhua Liu,Sidong Luo,Di Chen,Jinbo Lin,Man Xiong,Lei Yang,Kaifan Li,Dawei Sun,Lina Wei,Sheng Luo,Yeyang Wang

Frontiers in immunology 16:1600035 PubMed40799647

2025

Hypobaric hypoxia aggravates neuroinflammation in ligature-induced periodontitis mice via the STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Chen,Jiayi Zhang,Xuechao Fei,Jiajing Wang,Jiayue Gao,Xiangpei Yue,Yaqun Jiang,Zibi Shi,Shaojie Zhang,Xiufang Jiang,Wenyue Chang,Zhonghua Dai,Jianjun Guo,Tong Zhao,Xiaoxia Jiang,Lingling Zhu

Frontiers in cell and developmental biology 13:1611226 PubMed40530334

2025

Induced neural stem cells ameliorate blood-brain barrier injury by modulating the calcium signaling pathway of astrocyte in cerebral ischemia-reperfusion rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyun Liang,Chuanshang Cao,Ningmei Liu,Dongmei Chen,Ting Liu,Haibin Ma,Jiaxin Liu,Taojuan Wu,Jianguo Niu

Journal of translational medicine 23:579 PubMed40410771

2025

Injectable nanocomposite hydrogel for localized precision delivery of dexamethasone after traumatic brain injury: dual modulation of neuroinflammation and blood-brain barrier restoration.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Zhang,Miao Bai,Mengshi Yang,Yumei Wang,Xiyu Chen,Baiyun Liu,Guangzhi Shi

Experimental & molecular medicine 57:1064-1077 PubMed40335634

2025

Acetylation of α-tubulin restores endothelial cell injury and blood-brain barrier disruption after intracerebral hemorrhage in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejiao Lei,Eryi Sun,Xufang Ru,Yulian Quan,Xuezhu Chen,Qian Zhang,Yougling Lu,Qianying Huang,Yujie Chen,Wenyan Li,Hua Feng,Yang Yang,Rong Hu

Frontiers in molecular neuroscience 18:1511388 PubMed40303283

2025

Brain microvascular endothelial cells differentiated from a Friedreich's Ataxia patient iPSC are deficient in tight junction protein expression and paracellularly permeable.

Applications

Unspecified application

Species

Unspecified reactive species

Frances M Smith,Daniel J Kosman

Nature communications 16:3748 PubMed40258811

2025

Targeted immunotherapy rescues pulmonary fibrosis by reducing activated fibroblasts and regulating alveolar cell profile.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yan,Song-Yu Wang,Qi Su,Min-Wen Zou,Zi-Yue Zhou,Jian Shou,Yunlong Huo

Science advances 11:eads0505 PubMed40238886

2025

Amyotrophic lateral sclerosis and frontotemporal dementia mutation reduces endothelial TDP-43 and causes blood-brain barrier defects.

Applications

Unspecified application

Species

Unspecified reactive species

Ashok Cheemala,Amy L Kimble,Emily N Burrage,Stephen B Helming,Jordan D Tyburski,Nathan K Leclair,Omar M Omar,Aamir R Zuberi,Melissa Murphy,Evan R Jellison,Bo Reese,Xiangyou Hu,Cathleen M Lutz,Riqiang Yan,Patrick A Murphy
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com