AB172968

Anti-Claudin 5 (phospho Y217) antibody [EPR10810]

RabMAb
Recombinant
20ul selling size
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(8 Publications)

Rabbit Recombinant Monoclonal Claudin 5 phospho Y217 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 8 publications.

View Alternative Names

AWAL, TMVCF, CLDN5, Claudin-5, Transmembrane protein deleted in VCFS, TMDVCF

3 Images

Supplier Data

Immunoprecipitation - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (AB172968)

Western blot analysis on immunoprecipitation pellet from (1) MCF7 cells treated with pervanadate lysate or (2) 1X PBS (negative control) using ab172968 Phospho (pY217) at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968)

Predicted band size: 23 kDa

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Lab

Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (AB172968)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as a GAPDH loading control.

All lanes:

Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968) at 1/1000 dilution

Lane 1:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A431 (Human epidermoid carcinoma epithelial cell) treated with 50mM Pervanadate for 5 minutes whole cell lysate at 20 µg

Lane 3:

A431 (Human epidermoid carcinoma epithelial cell) treated with 50mM Pervanadate for 5 minutes whole cell lysate, the membrane was treated with alkaline phosphatase for 1 hour at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 20 kDa

false

Exposure time: 5s

Supplier Data

Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (AB172968)

All lanes:

Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968) at 1/50000 dilution

Lane 1:

MCF7 cell lysate, untreated at 10 µg

Lane 2:

MCF7 cell lysate, treated with pervanadate at 10 µg

Predicted band size: 23 kDa

false

Carrier free

Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10810

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin 5 also known as CLDN5 is an integral component of tight junction strands within endothelial and epithelial cells. This protein possesses a molecular weight of approximately 23 kDa. It prominently expresses in the vascular endothelial cells of the blood-brain barrier and other blood-tissue barriers. Claudin 5 plays a mechanical role in forming selectively permeable paracellular barriers that regulate the movement of molecules and ions between cells. This function is essential in preserving the tissue-specific environment.

Biological function summary

Claudin 5 serves as an important element in maintaining the integrity of the blood-brain barrier by creating a selective permeability. It operates as part of a larger tight junction complex interacting mainly with other claudin proteins and proteins such as occludin and ZO-1. These interactions ensure the structural and functional integrity of the cell junctions which helps stabilize the barrier properties against various physiological and pathological insults.

Pathways

Claudin 5 is involved in important biological pathways such as the tight junction pathway and the barrier permeability regulation pathway. Through these pathways it interacts with proteins like occludin and various signaling molecules. This involvement allows tight regulation of barrier permeability ensuring selective transport and preventing the passage of harmful substances into sensitive areas like the central nervous system.

Claudin 5 plays a significant role in conditions like cerebral edema and Alzheimer's disease. Altered expression or dysfunction of Claudin 5 disrupts the blood-brain barrier contributing to these disorders. In Alzheimer's disease there is evidence of impaired tight junctions where Claudin 5 interacts with other claudins and related proteins like amyloid-beta affecting barrier integrity. Understanding these connections helps in developing therapeutic strategies targeting tight junction stability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a major role in tight junction-specific obliteration of the intercellular space.

See full target information CLDN5 pY217

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1534729 PubMed40406486

2025

Protective effects of AER-271 in acute-phase radiation-induced brain injury in rats: reduction of brain edema, inflammation, apoptosis and maintenance of blood-brain barrier integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Yaozu Xiong,Yifei Wang,Mingyue Li,Changhua Yu,Yusuo Tong,Xiaoting Xu

Aging cell 23:e14106 PubMed38358083

2024

Homocysteine potentiates amyloid -induced death receptor 4- and 5-mediated cerebral endothelial cell apoptosis, blood brain barrier dysfunction and angiogenic impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley Carey,Rebecca Parodi-Rullan,Rafael Vazquez-Torres,Elisa Canepa,Silvia Fossati

Neural regeneration research 19:887-894 PubMed37843225

2023

Sustained release of vascular endothelial growth factor A and basic fibroblast growth factor from nanofiber membranes reduces oxygen/glucose deprivation-induced injury to neurovascular units.

Applications

Unspecified application

Species

Unspecified reactive species

Yifang Wu,Jun Sun,Qi Lin,Dapeng Wang,Jian Hai

Frontiers in pharmacology 13:908830 PubMed35814200

2022

Exosome-Like Nanoparticles Exert Neuroprotective Effects Against Ischemic Brain Injury Inhibiting Matrix Metalloproteinase 9 and Activating the AKT/GSK3β Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Cai,Lin-Yan Huang,Rui Hong,Jin-Xiu Song,Xin-Jian Guo,Wei Zhou,Zhao-Li Hu,Wan Wang,Yan-Ling Wang,Jian-Gang Shen,Su-Hua Qi

Aging 14:3554-3568 PubMed35471411

2022

Claudin-5 relieves cognitive decline in Alzheimer's disease mice through suppression of inhibitory GABAergic neurotransmission.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Zhu,Meidan Wei,Linguang Yuan,Xiaodan He,Chunli Chen,Aimin Ji,Guozeng Zhang

Frontiers in cellular neuroscience 16:841544 PubMed35308117

2022

Healthy Serum-Derived Exosomes Improve Neurological Outcomes and Protect Blood-Brain Barrier by Inhibiting Endothelial Cell Apoptosis and Reversing Autophagy-Mediated Tight Junction Protein Reduction in Rat Stroke Model.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Yan Huang,Jin-Xiu Song,Heng Cai,Pei-Pei Wang,Qi-Long Yin,Yi-De Zhang,Jie Chen,Ming Li,Jia-Jia Song,Yan-Ling Wang,Lan Luo,Wan Wang,Su-Hua Qi

Annals of translational medicine 9:1410 PubMed34733962

2021

miR-151-5p alleviates corneal allograft rejection by activating PI3K/AKT signaling pathway and balancing Th17/Treg after corneal transplantation via targeting IL-2Rɑ.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Cao,Yunchuan Li,Yong Li,Lan Li

American journal of translational research 10:827-836 PubMed29636872

2018

Qihuang decoction promotes the recovery of intestinal immune barrier dysfunction after gastrectomy in rats.

Applications

Species

Rat

Hui Peng,Yi Shen,Qi Zhang,Juda Liu,Zhen Wang,Long Huang,Fuhai Zhou,Jian Yu,Mingyang Liu,Yiyang Yuan,Shushan Yu,Qingsheng Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Claudin 5 (phospho Y217) antibody [EPR10810]

Immunoprecipitation - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (AB172968)

Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (AB172968)

Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (AB172968)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise