AB249739

Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal Claudin 5 phospho Y217 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

AWAL, TMVCF, CLDN5, Claudin-5, Transmembrane protein deleted in VCFS, TMDVCF

3 Images

Supplier Data

Immunoprecipitation - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free (AB249739)

This data was developed using ab172968, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (1) MCF7 cells treated with pervanadate lysate or (2) 1X PBS (negative control) using ab172968 Phospho (pY217) at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (<a href='/en-us/products/primary-antibodies/claudin-5-phospho-y217-antibody-epr10810-ab172968'>ab172968</a>)

Predicted band size: 23 kDa

false

Lab

Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free (AB249739)

This data was developed using ab172968, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as a GAPDH loading control.

All lanes:

Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (<a href='/en-us/products/primary-antibodies/claudin-5-phospho-y217-antibody-epr10810-ab172968'>ab172968</a>) at 1/1000 dilution

Lane 1:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A431 (Human epidermoid carcinoma epithelial cell) treated with 50mM Pervanadate for 5 minutes whole cell lysate at 20 µg

Lane 3:

A431 (Human epidermoid carcinoma epithelial cell) treated with 50mM Pervanadate for 5 minutes whole cell lysate, the membrane was treated with alkaline phosphatase for 1 hour at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 20 kDa

false

Exposure time: 5s

Supplier Data

Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free (AB249739)

This data was developed using ab172968, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (<a href='/en-us/products/primary-antibodies/claudin-5-phospho-y217-antibody-epr10810-ab172968'>ab172968</a>) at 1/50000 dilution

Lane 1:

MCF7 cell lysate, untreated at 10 µg

Lane 2:

MCF7 cell lysate, treated with pervanadate at 10 µg

Predicted band size: 23 kDa

false

Unconjugated

Anti-Claudin 5 (phospho Y217) antibody [EPR10810]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10810

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

ab249739 is the carrier-free version of ab172968.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin 5 also known as CLDN5 is an integral component of tight junction strands within endothelial and epithelial cells. This protein possesses a molecular weight of approximately 23 kDa. It prominently expresses in the vascular endothelial cells of the blood-brain barrier and other blood-tissue barriers. Claudin 5 plays a mechanical role in forming selectively permeable paracellular barriers that regulate the movement of molecules and ions between cells. This function is essential in preserving the tissue-specific environment.

Biological function summary

Claudin 5 serves as an important element in maintaining the integrity of the blood-brain barrier by creating a selective permeability. It operates as part of a larger tight junction complex interacting mainly with other claudin proteins and proteins such as occludin and ZO-1. These interactions ensure the structural and functional integrity of the cell junctions which helps stabilize the barrier properties against various physiological and pathological insults.

Pathways

Claudin 5 is involved in important biological pathways such as the tight junction pathway and the barrier permeability regulation pathway. Through these pathways it interacts with proteins like occludin and various signaling molecules. This involvement allows tight regulation of barrier permeability ensuring selective transport and preventing the passage of harmful substances into sensitive areas like the central nervous system.

Claudin 5 plays a significant role in conditions like cerebral edema and Alzheimer's disease. Altered expression or dysfunction of Claudin 5 disrupts the blood-brain barrier contributing to these disorders. In Alzheimer's disease there is evidence of impaired tight junctions where Claudin 5 interacts with other claudins and related proteins like amyloid-beta affecting barrier integrity. Understanding these connections helps in developing therapeutic strategies targeting tight junction stability.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Plays a major role in tight junction-specific obliteration of the intercellular space.

See full target information CLDN5 pY217

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free

Immunoprecipitation - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free (AB249739)

Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free (AB249739)

Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free (AB249739)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise