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AB75055

Anti-Claudin 6 antibody

2

(2 Reviews)

|

(3 Publications)

Rabbit Polyclonal Claudin 6 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CLDN6.

View Alternative Names

UNQ757/PRO1488, CLDN6, Claudin-6, Skullin

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Claudin 6 antibody (AB75055)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 6 antibody (AB75055)

Huvec cells with ab75055 at 1/500 dilution, in the absence and presence of the immunising peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 6 antibody (AB75055)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 6 antibody (AB75055)

Human ovarian tissue with ab75055 at 1/50 dilution, in the absence and presence of the immunising peptide.

Western blot - Anti-Claudin 6 antibody (AB75055)
  • WB

Unknown

Western blot - Anti-Claudin 6 antibody (AB75055)

All lanes:

Western blot - Anti-Claudin 6 antibody (ab75055) at 1/500 dilution

Lane 1:

extracts from Jurkat cells at 5 µg

Lane 2:

extracts from Jurkat cells at 5 µg with immunising peptide

Predicted band size: 23 kDa

Observed band size: 25 kDa,<19 kDa

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Western blot - Anti-Claudin 6 antibody (AB75055)
  • WB

Supplier Data

Western blot - Anti-Claudin 6 antibody (AB75055)

All lanes:

Western blot - Anti-Claudin 6 antibody (ab75055) at 1/500 dilution

All lanes:

Jurkat cell lysate

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human CLDN6. The exact immunogen used to generate this antibody is proprietary information.

P56747

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin 6 also known as CLDN6 is a transmembrane protein belonging to the claudin family. This family is critical in forming tight junctions between cells which regulate the paracellular barrier functions of epithelia. The molecular mass of claudin 6 is approximately 23 kDa. Researchers have observed its expression mainly in embryonic tissues and certain cancer types. In normal adult tissues it shows limited expression indicating a specific role during early developmental stages.
Biological function summary

Claudin 6 participates in the formation and maintenance of tight junctions in epithelial cells. It is part of a larger complex that includes multiple claudins and other tight junction proteins like occludin and zonula occludens-1. This complex maintains cellular polarity and controls the flow of ions and molecules between cells which is essential for tissue homeostasis. Its expression pattern suggests a role in cell differentiation and proliferation during embryogenesis and tumorigenesis.

Pathways

Various studies indicate that claudin 6 is involved in the Wnt signaling pathway which is key in embryonic development and cell differentiation. This pathway interacts with several other proteins including β-catenin to mediate gene expression and cellular functions. Additionally claudin 6 contributes to pathways influencing the epithelial-mesenchymal transition (EMT) a process necessary for organ development and cancer metastasis. Through these pathways claudin 6 interacts with diverse proteins to modulate cell behavior.

Researchers have linked claudin 6 to certain cancers particularly ovarian and breast cancer. Its aberrant expression may contribute to tumor progression and invasiveness. Claudin 6 can promote changes in the tumor microenvironment by interacting with other cell junction proteins possibly influencing cancer cell proliferation and spread. Furthermore its altered expression is noted in immune disorders suggesting potential roles in pathogenesis through interactions with immune cells or signaling molecules like cytokines within the immune pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a major role in tight junction-specific obliteration of the intercellular space.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.
See full target information CLDN6
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 38:e23682 PubMed38462752

2024

CLDN6 inhibited cellular biological function of nonsmall cell lung cancer cells through suppressing aerobic glycolysis via the RIP1/ASK1/JNK axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Guo,Jianying Li,Yu Dong,Humei Gao,Peng Wang

Gut 64:957-65 PubMed25063678

2014

Human hepatic stellate cells are not permissive for hepatitis C virus entry and replication.

Applications

Unspecified application

Species

Human

Alexandre Florimond,Philippe Chouteau,Patrice Bruscella,Jacques Le Seyec,Emilie Mérour,Nazim Ahnou,Ariane Mallat,Sophie Lotersztajn,Jean-Michel Pawlotsky

PloS one 8:e62159 PubMed23626783

2013

Differential expression of candidate virus receptors in human T lymphocytes prone or resistant to infection with patient-derived hepatitis C virus.

Applications

WB

Species

Human

Mohammed A Sarhan,Annie Y Chen,Tomasz I Michalak
View all publications
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