Anti-Claudin 6 antibody [EPR28103-113] 20 ul size (ab314134)

Rabbit Recombinant Monoclonal Claudin 6 antibody. Suitable for WB, IP, ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate - Human, Transfected cell line samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 6 antibody [EPR28103-113] (AB314134), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IP	ICC/IF	IHC-P	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested
Transfected cell line	Not recommended	Not recommended	Not recommended	Tested	Not recommended
Transfected cell lysate - Human	Tested	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Transfected cell lysate - Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human, Transfected cell line	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human, Transfected cell line	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Transfected cell line	Dilution info 1/100 - 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human, Transfected cell line	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information CLDN6

Function

Plays a major role in tight junction-specific obliteration of the intercellular space. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.

Alternative names

UNQ757/PRO1488, CLDN6, Claudin-6, Skullin

Recommended products

Rabbit Recombinant Monoclonal Claudin 6 antibody. Suitable for WB, IP, ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate - Human, Transfected cell line samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28103-113
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Claudin 6 also known as CLDN6 is a transmembrane protein belonging to the claudin family. This family is critical in forming tight junctions between cells which regulate the paracellular barrier functions of epithelia. The molecular mass of claudin 6 is approximately 23 kDa. Researchers have observed its expression mainly in embryonic tissues and certain cancer types. In normal adult tissues it shows limited expression indicating a specific role during early developmental stages.

Biological function summary

Claudin 6 participates in the formation and maintenance of tight junctions in epithelial cells. It is part of a larger complex that includes multiple claudins and other tight junction proteins like occludin and zonula occludens-1. This complex maintains cellular polarity and controls the flow of ions and molecules between cells which is essential for tissue homeostasis. Its expression pattern suggests a role in cell differentiation and proliferation during embryogenesis and tumorigenesis.

Pathways

Various studies indicate that claudin 6 is involved in the Wnt signaling pathway which is key in embryonic development and cell differentiation. This pathway interacts with several other proteins including β-catenin to mediate gene expression and cellular functions. Additionally claudin 6 contributes to pathways influencing the epithelial-mesenchymal transition (EMT) a process necessary for organ development and cancer metastasis. Through these pathways claudin 6 interacts with diverse proteins to modulate cell behavior.

Associated diseases and disorders

Researchers have linked claudin 6 to certain cancers particularly ovarian and breast cancer. Its aberrant expression may contribute to tumor progression and invasiveness. Claudin 6 can promote changes in the tumor microenvironment by interacting with other cell junction proteins possibly influencing cancer cell proliferation and spread. Furthermore its altered expression is noted in immune disorders suggesting potential roles in pathogenesis through interactions with immune cells or signaling molecules like cytokines within the immune pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 6 antibody [EPR28103-113] (ab314134)
Immunohistochemical analysis of formalin-fixed paraffin-embedded 293T (human embryonic kidney epithelial cells) labelling CLDN6 with ab314134 at a concentration of 0.1 µg/ml in cells transfected with over expression vectors containing a His tag.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with a Bond™ Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.
ab314134 anti-CLDN6 antibody [EPR28103-113] was incubated for 15 mins at room temperature. Cells transfected with an empty vector showed no staining. Absence of staining was observed when stained using anti-rabbit IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) or secondary antibody only control. Sections were counterstained with Hematoxylin.
Western blot - Anti-Claudin 6 antibody [EPR28103-113] (ab314134)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: DU 145

Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: 180 seconds
All lanes: Western blot - Anti-Claudin 6 antibody [EPR28103-113] (ab314134) at 1/1000 dilution
Lane 1: NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: JEG-3 (human placenta epithelial cell) whole cell lysate at 20 µg
Lane 3: JAR (human placenta choriocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: DU 145 (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 23 kDa
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 6 antibody [EPR28103-113] (ab314134)
Immunohistochemical analysis of paraffin-embedded Tissue Microarray (T tissue labeling Claudin 6 with ab314134 at 1/100 (5.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Table of the human normal TMA tissue and human tumor tissue TMA depending on the staining. The section was incubated with ab314134 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Immunocytochemistry/ Immunofluorescence - Anti-Claudin 6 antibody [EPR28103-113] (ab314134)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) cells labelling Claudin 6 with ab314134 at 1/50 (10.32 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous staining in NIH:OVCAR-3 cells. Negative control: DU 145. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 6 antibody [EPR28103-113] (ab314134)
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human tissue labeling Claudin 6 with ab314134 at 1/5000 (0.103 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a His-tagged Claudin-6 (CLDN6) cell pellet; no staining on (B) HEK-293T transfected with a His-tagged Claudin-9 (CLDN9) construct cell pellet and (C) HEK-293T transfected with a His-tagged empty vector cell pellet. The section was incubated with ab314134 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Flow Cytometry (Intracellular) - Anti-Claudin 6 antibody [EPR28103-113] (ab314134)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized DU 145 (human prostate carcinoma epithelial cell, Left) / NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell, Right) cells labelling Claudin 6 with ab314134 at 1/50 dilution (1 ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control: DU 145.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 6 antibody [EPR28103-113] (ab314134)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Claudin 6 with ab314134 at 1/100 (5.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human skeletal muscle (PMID: 31896660). The section was incubated with ab314134 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 6 antibody [EPR28103-113] (ab314134)
Immunohistochemical analysis of paraffin-embedded (A) NIH OVCAR-3 (hum tissue labeling Claudin 6 with ab314134 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) NIH:OVCAR-3 cell pellet; no staining on (B) DU 145 cell pellet. The section was incubated with ab314134 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 6 antibody [EPR28103-113] (ab314134)
Immunohistochemical analysis of paraffin-embedded human testis seminoma tissue labeling Claudin 6 with ab314134 at 1/100 (5.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human testis seminoma (PMID: 22803571). The section was incubated with ab314134 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 6 antibody [EPR28103-113] (ab314134)
Immunohistochemical analysis of paraffin-embedded human ovarian adenocarcinoma tissue labeling Claudin 6 with ab314134 at 1/100 (5.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human ovarian adenocarcinoma. The section was incubated with ab314134 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Western blot - Anti-Claudin 6 antibody [EPR28103-113] (ab314134)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human CLDN9.

Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution
Exposure time: 3 seconds
All lanes: Western blot - Anti-Claudin 6 antibody [EPR28103-113] (ab314134) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a His tag whole cell lysate at 10 µg
Lane 2: 293T cells transfected with a human CLDN6 expression vector containing a His-tag whole cell lysate at 10 µg
Lane 3: 293T cells transfected with a human CLDN9 expression vector containing a His-tag whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 23 kDa
Exposure time: 3s
Immunoprecipitation - Anti-Claudin 6 antibody [EPR28103-113] (ab314134)
Claudin 6 was immunoprecipitated from 0.35 mg NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) whole cell lysate with ab314134 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314134 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) whole cell lysate
Lane 2: NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314134 in NIH:OVCAR-3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds
All lanes: Immunoprecipitation - Anti-Claudin 6 antibody [EPR28103-113] (ab314134) at 1/30 dilution
All lanes: NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 15s