AB265583

Anti-Claudin 7/CLDN-7 antibody

Name: Anti-Claudin 7/CLDN-7 antibody (ab265583) | Abcam
Brand: Abcam Limited
SKU: AB265583

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(1 Publication)

Rabbit Polyclonal Claudin 7 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CLDN7 aa 150 to C-terminus.

View Alternative Names

CEPTRL2, CPETRL2, CLDN7, Claudin-7, CLDN-7

2 Images

Supplier Data

Immunoprecipitation - Anti-Claudin 7/CLDN-7 antibody (AB265583)

CLDN7 was immuoprecipitated from 1 mg MCF7 whole cell lysate using ab265583 at 40 μL per reaction. Western blot was performed on the immunoprecipitate using ab265583 at 1/1000 dilution.

Lane 1 : ab265583 IP in MCF7 whole cell lysate.
Lane 2 : Control IgG.

All lanes:

Immunoprecipitation - Anti-Claudin 7/CLDN-7 antibody (ab265583)

Predicted band size: 22 kDa

true

Exposure time: 10s

Supplier Data

Western blot - Anti-Claudin 7/CLDN-7 antibody (AB265583)

All lanes:

Western blot - Anti-Claudin 7/CLDN-7 antibody (ab265583) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 15 µg

Lane 2:

LNCap whole cell lysate at 15 µg

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 15 µg

Predicted band size: 22 kDa

true

Exposure time: 75s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human CLDN7 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95471

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>20-40 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin 7 often referred to as CLDN-7 is a transmembrane protein with a molecular mass of approximately 22 kDa. It is part of the claudin family which includes other members like claudin-1 and claudin-3. CLDN-7 is prominently found in epithelial cells and plays an important role in forming tight junctions between cells. These tight junctions are integral in maintaining cell polarity and controlling the paracellular transport of ions and molecules.

Biological function summary

Claudin 7 contributes to the integrity and function of tight junctions by directly interacting with other claudins and zonula occludens-1 (ZO-1) thereby becoming part of a larger complex. This structure regulates the movement of substances between epithelial cells protecting tissues from pathogens and maintaining homeostasis. Claudin 7 is essential for proper barrier function in tissues like the respiratory tract and renal structures.

Pathways

The function of claudin 7 integrates within the epithelial barrier signaling pathway and the Wnt signaling pathway. Through these pathways CLDN-7 interacts with proteins like β-catenin. In the epithelial barrier pathway CLDN-7 helps maintain cellular adhesion and barrier function while in the Wnt pathway it might influence cell proliferation and differentiation indirectly through associated proteins.

Claudin 7 has a significant connection with certain cancers such as gastric and colorectal cancer. Alterations in CLDN-7 expression can disrupt cell adhesion leading to increased invasiveness of cancer cells. It is often observed that in these cancers changes in CLDN-7 expression correlate with E-cadherin another key protein in maintaining tissue architecture and integrity. Additionally variations in CLDN-7 are linked to renal disorders where disrupted tight junctions can affect kidney function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a major role in tight junction-specific obliteration of the intercellular space.

See full target information CLDN7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:7 PubMed34815759

2021

IRF2-induced Claudin-7 suppresses cell proliferation, invasion and migration of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Weidong Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Claudin 7/CLDN-7 antibody

Immunoprecipitation - Anti-Claudin 7/CLDN-7 antibody (AB265583)

Western blot - Anti-Claudin 7/CLDN-7 antibody (AB265583)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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