Rabbit Polyclonal Claudin 7 antibody. Suitable for IHC-P, IHC-Fr, WB, ICC/IF, ICC and reacts with Human, Mouse, Rat samples. Cited in 40 publications.
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- Microbiology spectrum 10:e02124212022Limosilactobacillus reuteri SLZX19-12 Protects the Colon from Infection by Enhancing Stability of the Gut Microbiota and Barrier Integrity and Reducing Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianmin Wu,Zishen Lin,Xian Wang,Ying Zhao,Jinbiao Zhao,Hu Liu,Lee J Johnston,Lin Lu,Xi MaPubMed 35658572
- Journal of oncology 2022:27501932022Digital Image Analysis-Based Evaluation of Claudin-1 and Claudin-7 Delocalization in Cutaneous Squamous Cell Carcinoma and in Its Precancerous State.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLina Xu,Yunlei Pan,Shunli Tang,Juan Bai,Yinhua Wu,Jianjun Qiao,Hong FangPubMed 35432533
- Frontiers in cellular and infection microbiology 12:8245472022Serine Protease Degrades Several Protein Components of Tight Junctions and Assists Bacterial Translocation Across the T84 Monolayer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMitsunobu Ueda,Hidetomo Kobayashi,Soshi Seike,Eizo Takahashi,Keinosuke Okamoto,Hiroyasu YamanakaPubMed 35273923
- Molecular human reproduction 27:2021Uterine kisspeptin receptor critically regulates epithelial estrogen receptor α transcriptional activity at the time of embryo implantation in a mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer Schaefer,Angelos G Vilos,George A Vilos,Moshmi Bhattacharya,Andy V BabwahPubMed 34524460
- Oncoimmunology 10:19239102021Cldn-7 deficiency promotes experimental colitis and associated carcinogenesis by regulating intestinal epithelial integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKun Wang,Yuhan Ding,Chang Xu,Mengdi Hao,Huimin Li,Lei DingPubMed 34026335
- Cancer biology & therapy 21:1154-11622020Desmoglein-2 as a prognostic and biomarker in ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiho Kim et. al.PubMed 33218274
- International journal of molecular sciences 21:2020Adipose Tissue from Lean and Obese Mice Induces a Mesenchymal to Epithelial Transition-Like Effect in Triple Negative Breast Cancers Cells Grown in 3-Dimensional Culture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmmanuel C Asante et. al.PubMed 32899433
- World journal of clinical cases 8:2190-22002020Evaluation of clinical significance of claudin 7 and construction of prognostic grading system for stage II colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-Chuan Quan,Jian Peng,Xu Guan,Zheng Liu,Zheng Jiang,Hai-Peng Chen,Meng Zhuang,Song Wang,Peng Sun,Hong-Ying Wang,Shuang-Mei Zou,Xi-Shan WangPubMed 32548149
- Nature communications 11:25402020Epithelial plasticity can generate multi-lineage phenotypes in human and murine bladder cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn P Sfakianos et. al.PubMed 32439865
- Developmental cell 52:413-428.e62020Single-Chromosomal Gains Can Function as Metastasis Suppressors and Promoters in Colon Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnand Vasudevan,Prasamit S Baruah,Joan C Smith,Zihua Wang,Nicole M Sayles,Peter Andrews,Jude Kendall,Justin Leu,Narendra Kumar Chunduri,Dan Levy,Michael Wigler,Zuzana Storchová,Jason M SheltzerPubMed 32097652
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IHC-P | IHC-Fr | WB | ICC/IF | ICC | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected | Predicted |
| Mouse | Expected | Expected | Expected | Expected | Expected |
| Rat | Expected | Expected | Expected | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min then cool at room temperature for 20 min. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200 | Notes In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min then cool at room temperature for 20 min. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/200 | Notes In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min then cool at room temperature for 20 min. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Plays a major role in tight junction-specific obliteration of the intercellular space.
Alternative names
Claudin-7, CLDN-7, CPETRL2, CEPTRL2, CLDN7
Recommended products
Rabbit Polyclonal Claudin 7 antibody. Suitable for IHC-P, IHC-Fr, WB, ICC/IF, ICC and reacts with Human, Mouse, Rat samples. Cited in 40 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
Ab27487 is affinity purified.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Claudin 7 often referred to as CLDN-7 is a transmembrane protein with a molecular mass of approximately 22 kDa. It is part of the claudin family which includes other members like claudin-1 and claudin-3. CLDN-7 is prominently found in epithelial cells and plays an important role in forming tight junctions between cells. These tight junctions are integral in maintaining cell polarity and controlling the paracellular transport of ions and molecules.
- Biological function summary
Claudin 7 contributes to the integrity and function of tight junctions by directly interacting with other claudins and zonula occludens-1 (ZO-1) thereby becoming part of a larger complex. This structure regulates the movement of substances between epithelial cells protecting tissues from pathogens and maintaining homeostasis. Claudin 7 is essential for proper barrier function in tissues like the respiratory tract and renal structures.
- Pathways
The function of claudin 7 integrates within the epithelial barrier signaling pathway and the Wnt signaling pathway. Through these pathways CLDN-7 interacts with proteins like β-catenin. In the epithelial barrier pathway CLDN-7 helps maintain cellular adhesion and barrier function while in the Wnt pathway it might influence cell proliferation and differentiation indirectly through associated proteins.
- Associated diseases and disorders
Claudin 7 has a significant connection with certain cancers such as gastric and colorectal cancer. Alterations in CLDN-7 expression can disrupt cell adhesion leading to increased invasiveness of cancer cells. It is often observed that in these cancers changes in CLDN-7 expression correlate with E-cadherin another key protein in maintaining tissue architecture and integrity. Additionally variations in CLDN-7 are linked to renal disorders where disrupted tight junctions can affect kidney function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody (ab27487)
ab27487, at a dilution of 1/200, staining Claudin 7/CLDN-7 in formalin fixed paraffin embedded human colon carcinoma tissue section by Immunohistochemistry.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com