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AB250704

Anti-Claudin 8 antibody [EPR12680(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Claudin 8 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

UNQ779/PRO1573, CLDN8, Claudin-8

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 8 antibody [EPR12680(2)] - BSA and Azide free (AB250704)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 8 antibody [EPR12680(2)] - BSA and Azide free (AB250704)

This data was developed using ab183738, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Claudin 8 with ab183738 at 1/1000 dilution. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 8 antibody [EPR12680(2)] - BSA and Azide free (AB250704)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 8 antibody [EPR12680(2)] - BSA and Azide free (AB250704)

This data was developed using ab183738, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling Claudin 8 with ab183738 at 1/1000 dilution. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12680(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab250704 is the carrier-free version of ab183738.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin 8 also known as CLDN8 is a protein that functions as a critical component of tight junction strands which are integral to maintaining cell-to-cell adhesion. This protein belongs to the claudin family characterized by their role in forming the backbone of tight junctions. Claudin 8 has a molecular mass of approximately 23 kDa. It is expressed in several tissues including the kidney and gastrointestinal tract epithelium where it regulates selective permeability.
Biological function summary

Claudin 8 contributes to the creation and maintenance of the barrier function of epithelial layers. It integrates into tight junction complexes collaborating with other claudins and occludin to establish selective paracellular transport. This organization allows for the regulation of movement of ions and small solutes between cells essential in controlling the internal environment of organs.

Pathways

Research shows Claudin 8 plays a role in epithelial and endothelial cell permeability. It is involved in the calcium signaling pathway and the MAPK (Mitogen-Activated Protein Kinase) pathway both important for cell signaling and response to extracellular stimuli. Within these pathways Claudin 8 interacts with proteins such as ZO-1 (Zonula Occludens-1) to modulate tight junction integrity and function.

Defects or changes in Claudin 8 expression have associations with conditions like hypermagnesemia with seizures and mental retardation as well as certain types of cancer. In these contexts alterations in the protein's function can disrupt normal paracellular transport and contribute to disease pathology. Additionally Claudin 8 also links with occludin another component of tight junctions suggesting that aberrant interactions between these proteins might underpin certain disease processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Can associate with other claudins to regulate tight junction structural and functional strand dynamics (By similarity). May coassemble with CLDN4 into tight junction strands containing anion-selective channels that convey paracellular chloride permeability in renal collecting ducts (By similarity) (PubMed : 36008380). Cannot form tight junction strands on its own (PubMed : 36008380).
See full target information CLDN8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 5: PubMed31094704

2019

Protective role of B cells in sterile particulate-induced lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shaikh M Atif,Douglas G Mack,Amy S McKee,Javier Rangel-Moreno,Allison K Martin,Andrew Getahun,Lisa A Maier,John C Cambier,Rubin Tuder,Andrew P Fontenot
View all publications
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Product promise

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