Anti-Claudin 8 antibody [RP23040053]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 8 antibody [RP23040053] (AB308007)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 90% confluent log phase Caco-2 cells labelling Claudin-8 with ab308007 at 1/400 dilution, followed by Alexa Fluor® 488 Goat anti-Rabbit IgG secondary antibody at a dilution of 1 : 400 (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. Panel c is a merged image showing cell junction localization and panel d is a control without primary antibody. The images were captured using a Nikon microscope at 20X magnification.

• WB

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Western blot - Anti-Claudin 8 antibody [RP23040053] (AB308007)

Western blot analysis of Claudin-8 in HeLa (lane 1) and HEK293 (lane 2) whole cell extracts using ab308007 at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~25kDa.

All lanes:

Western blot - Anti-Claudin 8 antibody [RP23040053] (ab308007) at 2 µg/mL

Lane 1:

HeLa whole cell lysate

Lane 2:

HEK293 whole cell lysate

Observed band size: 25 kDa

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• WB

Supplier Data

Western blot - Anti-Claudin 8 antibody [RP23040053] (AB308007)

Western blot analysis of Claudin-8 was performed by loading 20 µg of Jurkat, U-2 OS, HEK-293, U-87 MG, K562 and A431 cell lysates were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. and blocked with 5% skim milk for 1 hour at room temperature. Claudin-8 was detected at ~27 kDa using ab308007 at a 1 : 1000 dilution in 2.5% skim milk at 4°C overnight on a rocking platform. Detection was performed using an HRP-conjugated Goat anti-Rabbit secondary antibody at a 1 : 5000 dilution and chemiluminescent detection (ECL).

All lanes:

Western blot - Anti-Claudin 8 antibody [RP23040053] (ab308007) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

U-2-OS cell lysate at 20 µg

Lane 3:

HEK-293 cell lysate at 20 µg

Lane 4:

U-87 MG cell lysate at 20 µg

Lane 5:

K562 cell lysate at 20 µg

Lane 6:

A431 cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit secondary antibody at 1/5000 dilution

Observed band size: 27 kDa

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