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Rat Monoclonal Claudin 9 antibody. Carrier free. Suitable for Flow Cyt, cELISA and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Claudin 9.


Images

Flow Cytometry - Anti-Claudin 9 antibody [YD4E9] - BSA and Azide free (AB187116), expandable thumbnail

Key facts

Isotype

IgG2b

Host species

Rat

Storage buffer

pH: 7.2
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human Claudin 9. Database link O95484

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytcELISA
Human
Tested
Expected
Mouse
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1.2 µg for 106 Cells

Notes

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.

Alternative names

Recommended products

Rat Monoclonal Claudin 9 antibody. Carrier free. Suitable for Flow Cyt, cELISA and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Claudin 9.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human Claudin 9. Database link O95484
Carrier free

Yes

Clone number

YD4E9

Purification technique

Affinity purification Protein G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Claudin-9 also known as CLDN9 is a member of the claudin protein family. This protein plays an important role in forming tight junctions which are responsible for regulating paracellular permeability in epithelial and endothelial cell sheets. Claudin-9 has a predicted mass of approximately 23 kDa. It is primarily expressed in the inner ear specifically in the cochlea and has vital functions in maintaining the permeability barrier and the structural integrity of cell layers.

Biological function summary

Claudin-9 engages in maintaining the tight junction barrier function of epithelial cells. It operates within a complex interacting closely with other claudin family members to establish the selective paracellular barrier important for ion balance and cellular homeostasis. These interactions allow claudin-9 to contribute significantly to the physiology of tissues where it is expressed impacting hearing function through ion permeability regulation in the cochlear epithelium.

Pathways

Claudin-9 is an integral component of pathways involved in cell-to-cell adhesion and cell polarity maintenance. It participates prominently in the tight junction pathway which is tightly regulated for maintaining the fundamental structure of epithelial layers. In this context it works alongside related proteins such as occludin and certain zonula occludens proteins which fortify the overall structure and signaling pathways essential for epithelial cell function.

Associated diseases and disorders

Mutations or dysregulation of claudin-9 have been implicated in particular hearing impairments. Sensorineural hearing loss a condition attributed to malfunction in the inner ear's sensory parts aligns with malfunctions in claudin-9 activity. This condition can also have connections to other proteins involved in cochlear biology such as K+ channel proteins indicating how disruptions in tight junction function can affect ion homeostasis and lead to auditory dysfunction.

Product promise

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1 product image

  • Flow Cytometry - Anti-Claudin 9 antibody [YD4E9] - BSA and Azide free (ab187116), expandable thumbnail

    Flow Cytometry - Anti-Claudin 9 antibody [YD4E9] - BSA and Azide free (ab187116)

    Flow cytometry analysis of BOSC23 cells transiently transfected with an expression vector encoding either Claudin 9 (red curve) or a control transfectant; using ab187116 at 1.2 μg/106 cells. PE-conjugated secondary antibodies were used for the analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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