AB203563

Anti-Claudin18 antibody [34H14L15]

Name: Anti-Claudin18 antibody [34H14L15] (ab203563) | Abcam
Brand: Abcam Limited
SKU: AB203563
Price: 520 USD
Availability: InStock
Rating: 5 (2 reviews)

Recombinant
What is this?

(2 Reviews)

(10 Publications)

Anti-Claudin18 antibody [34H14L15] (ab203563) is a rabbit monoclonal antibody detecting Claudin18 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Recombinant format for unrivalled batch-batch consistency

View Alternative Names

UNQ778/PRO1572, CLDN18, Claudin-18

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [34H14L15] (AB203563)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [34H14L15] (AB203563)

Formalin-fixed, paraffin-embedded human stomach tissue stained for Claudin18 with ab203563 at 2 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Immunocytochemistry - Anti-Claudin18 antibody [34H14L15] (AB203563)

Immunofluorescent analysis of Caco-2 (Human colorectal adenocarcinoma cell line) cells (4% paraformaldehyde-fixed) labeling Claudin18 with ab203563 at 1/500 dilution followed with an Alexa Fluor^® 488 goat anti-rabbit IgG secondary antibody at 1/400 dilution (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. Panel c is a merged image showing cell junction localization and panel d is a control without primary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [34H14L15] (AB203563)

Formalin-fixed, paraffin-embedded human squamous lung carcinoma tissue stained for Claudin18 with ab203563 at 2 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Claudin18 antibody [34H14L15] (AB203563)

4-12% Bis-Tris gel

All lanes:

Western blot - Anti-Claudin18 antibody [34H14L15] (ab203563) at 1/1000 dilution

Lane 1:

Rat heart lysate at 30 µg

Lane 2:

A431 (Human epidermoid carcinoma cell line) cell lysate at 30 µg

Lane 3:

A549 (Human lung carcinoma cell line) cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/5000 dilution

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-Claudin18 antibody [34H14L15] (AB203563)

All lanes:

Western blot - Anti-Claudin18 antibody [34H14L15] (ab203563) at 2 µg/mL

Lane 1:

Mouse lung lysate

Lane 2:

Mouse lung lysate with immunizing peptide

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

34H14L15

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC, ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CLDN18 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

P56856

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/1000", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-3 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-3 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-3 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Claudin18 antibody [34H14L15] (ab203563) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Claudin18?
Anti-Claudin18 [34H14L15] (ab203563) specifically detects a band for Claudin18 (UniProt: P56856) at a molecular weight of 28kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin-18 often noted as Claudin18 or CLDN18 is a tight junction protein belonging to the Claudin family. The protein comes in different isoforms including Claudin 18.2 each having a distinct function and expression pattern. With an estimated molecular mass of approximately 24 kDa it is mainly expressed in epithelial cells of the stomach and specific lung epithelia. Researchers utilize it frequently in immunohistochemistry (IHC) studies often referred to as CLDN18 IHC for its role in maintaining cell-to-cell adhesion in these tissues.

Biological function summary

Claudin-18.2 integrates itself into a tight junction complex contributing to the selective permeability barrier in epithelial tissues. This barrier is essential for maintaining tissue homeostasis and protecting underlying tissue from various external factors. It plays a significant role in ion selectivity and barrier assurance of the epithelial cells-gastrointestinal site especially. The regulated expression and function of Claudin 18 are critical for effective epithelial barrier function.

Pathways

Claudin-18 is involved in pathways concerning epithelial morphogenesis and integrity. It aligns with occludins and other claudins to control paracellular transport and cellular integrity within these pathways. Through its association with proteins such as ZO-1 Claudin-18 ensures proper cell signaling and tight junction formation. Its regulation in epithelial cells contributes significantly to pathways that sustain cell structure and prevent uncontrolled leakage of solutes.

The altered expression of Claudin-18.2 is closely linked to gastric and lung cancers. Abnormal levels can disrupt the epithelial barrier contributing to tumor progression and metastasis. In gastric cancer altered Claudin-18 couples with overexpression of other proteins such as HER2 playing a role in the disease's aggressiveness. Researchers consider it a potential target for therapeutic interventions making Claudin-18.2 purchase or acquisition relevant for studies oriented toward cancer diagnostics and treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in alveolar fluid homeostasis via regulation of alveolar epithelial tight junction composition and therefore ion transport and solute permeability, potentially via downstream regulation of the actin cytoskeleton organization and beta-2-adrenergic signaling (By similarity). Required for lung alveolarization and maintenance of the paracellular alveolar epithelial barrier (By similarity). Acts to maintain epithelial progenitor cell proliferation and organ size, via regulation of YAP1 localization away from the nucleus and thereby restriction of YAP1 target gene transcription (By similarity). Acts as a negative regulator of RANKL-induced osteoclast differentiation, potentially via relocation of TJP2/ZO-2 away from the nucleus, subsequently involved in bone resorption in response to calcium deficiency (By similarity). Mediates the osteoprotective effects of estrogen, potentially via acting downstream of estrogen signaling independently of RANKL signaling pathways (By similarity).. Isoform A1. Involved in the maintenance of homeostasis of the alveolar microenvironment via regulation of pH and subsequent T-cell activation in the alveolar space, is therefore indirectly involved in limiting C.neoformans infection.. Isoform A2. Required for the formation of the gastric paracellular barrier via its role in tight junction formation, thereby involved in the response to gastric acidification.

See full target information CLDN18

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 73:82 PubMed38554200

2024

DR30318, a novel tri-specific T cell engager for Claudin 18.2 positive cancers immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Ma,Zhenxing Zhou,Wenwen Duan,Gaofeng Yao,Shimei Sheng,Sidou Zong,Xin Zhang,Changkui Li,Yuanyuan Liu,Fengting Ou,Maha Raja Dahar,Yanshan Huang,Lushan Yu

ACS pharmacology & translational science 6:1829-1840 PubMed38093841

2023

Screening, Construction, and Preliminary Evaluation of CLDN18.2-Specific Peptides for Noninvasive Molecular Imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Zilei Wang,Chuanke Zhao,Jin Ding,Yan Chen,Jiayue Liu,Xingguo Hou,XiangXing Kong,Bin Dong,Zhi Yang,Hua Zhu

JCI insight 8: PubMed36821371

2023

EPIREGULIN creates a developmental niche for spatially organized human intestinal enteroids.

Applications

Unspecified application

Species

Unspecified reactive species

Charlie J Childs,Emily M Holloway,Caden W Sweet,Yu-Hwai Tsai,Angeline Wu,Abigail Vallie,Madeline K Eiken,Meghan M Capeling,Rachel K Zwick,Brisa Palikuqi,Coralie Trentesaux,Joshua H Wu,Oscar Pellón-Cardenas,Charles J Zhang,Ian Glass,Claudia Loebel,Qianhui Yu,J Gray Camp,Jonathan Z Sexton,Ophir D Klein,Michael P Verzi,Jason R Spence

Toxins 14: PubMed36136552

2022

Characterization of Lung Injury following Abrin Pulmonary Intoxication in Mice: Comparison to Ricin Poisoning.

Applications

Unspecified application

Species

Unspecified reactive species

Anita Sapoznikov,Yoav Gal,Ron Alcalay,Yentl Evgy,Tamar Sabo,Chanoch Kronman,Reut Falach

Cancer medicine 12:1441-1450 PubMed35861118

2022

Membranous and nuclear staining of CLDN18 in HPV-independent and HPV-associated endocervical adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuzhen Du,Yanjiao Hu,Xiaoyu Ji,Lei Sui,Qingmei Zheng,Kejuan Song,Teng Lv,Yulong Chen,Han Zhao,Shuzhen Dai,Peng Zhao,Qin Yao

Frontiers in physiology 12:637790 PubMed33868003

2021

The Degradation of Airway Epithelial Tight Junctions in Asthma Under High Airway Pressure Is Probably Mediated by Piezo-1.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Zhou,Xiang-Dong Zhou,Rui Xu,Xian-Zhi Du,Qi Li,Bin Li,Guo-Yue Zhang,Ling-Xiu Chen,Juliy M Perelman,Victor P Kolosov

JCI insight 5: PubMed33021967

2020

Alveolar barrier disruption in varicella pneumonia is associated with neutrophil extracellular trap formation.

Applications

Unspecified application

Species

Unspecified reactive species

Werner Jd Ouwendijk,Henk-Jan van den Ham,Mark W Delany,Jeroen Ja van Kampen,Gijsbert P van Nierop,Tamana Mehraban,Fatiha Zaaraoui-Boutahar,Wilfred Fj van IJcken,Judith Ma van den Brand,Rory D de Vries,Arno C Andeweg,Georges Mgm Verjans

American journal of physiology. Lung cellular and molecular physiology 316:L255-L268 PubMed30382767

2018

Early disruption of the alveolar-capillary barrier in a ricin-induced ARDS mouse model: neutrophil-dependent and -independent impairment of junction proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Anita Sapoznikov,Yoav Gal,Reut Falach,Irit Sagi,Sharon Ehrlich,Elad Lerer,Arik Makovitzki,Anna Aloshin,Chanoch Kronman,Tamar Sabo

Nature 550:529-533 PubMed29019984

2017

Transitional basal cells at the squamous-columnar junction generate Barrett's oesophagus.

Applications

IHC-Fr

Species

Unspecified reactive species

Ming Jiang,Haiyan Li,Yongchun Zhang,Ying Yang,Rong Lu,Kuancan Liu,Sijie Lin,Xiaopeng Lan,Haikun Wang,Han Wu,Jian Zhu,Zhongren Zhou,Jianming Xu,Dong-Kee Lee,Lanjing Zhang,Yuan-Cho Lee,Jingsong Yuan,Julian A Abrams,Timothy C Wang,Antonia R Sepulveda,Qi Wu,Huaiyong Chen,Xin Sun,Junjun She,Xiaoxin Chen,Jianwen Que

Molecular medicine reports 15:2765-2787 PubMed28447763

2017

Berberine protects against diet-induced obesity through regulating metabolic endotoxemia and gut hormone levels.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Hui Xu,Xing Zhen Liu,Wei Pan,Da Jin Zou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Claudin18 antibody [34H14L15]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [34H14L15] (AB203563)

Immunocytochemistry - Anti-Claudin18 antibody [34H14L15] (AB203563)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [34H14L15] (AB203563)

Western blot - Anti-Claudin18 antibody [34H14L15] (AB203563)

Western blot - Anti-Claudin18 antibody [34H14L15] (AB203563)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise