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AB314691

Anti-Claudin18 antibody [43-14A] - BSA and Azide free

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(2 Publications)

Anti-Claudin18 antibody [43-14A] - BSA and Azide free (ab314691) is a mouse recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Claudin18 in IHC-P. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

UNQ778/PRO1572, CLDN18, Claudin-18

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (AB314691)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (AB314691)

This data was developed using ab314690, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Claudin18.2 expression vector containing a His tag. (B) HEK-293T transfected with a Claudin18.1 expression vector containing a His tag. (C) HEK-293T transfected with empty vector containing a His tag. tissue labeling Claudin18 with ab314690 at 1/10000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a Claudin18.2 expression vector containing a His tag and (B) HEK-293T transfected with a Claudin18.1 expression vector containing a His tag. No staining on (C) HEK-293T transfected with empty vector containing a His tag. The section was incubated with ab314690 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (AB314691)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (AB314691)

This data was developed using ab314690, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Claudin18 with ab314690 at 1/5000 (0.2096 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human pancreas. The section was incubated with ab314690 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (AB314691)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (AB314691)

This data was developed using ab314690, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Claudin18 with ab314690 at 1/5000 (0.2096 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human stomach. The section was incubated with ab314690 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (AB314691)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (AB314691)

This data was developed using ab314690, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling Claudin18 with ab314690 at 1/5000 (0.2096 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human gastric adenocarcinoma. The section was incubated with ab314690 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

43-14A

Isotype

IgG2b

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Cross-reactivity has been checked and this antibody might cross with Claudin18.1 and Claudin18.2.
The human gene CLDN18 has two protein isoforms, Claudin 18.1 and Claudin 18.2. The two isoforms differ within the N-terminal 69 amino acids. Claudin 18.1 expression is restricted to the lung. In contrast Claudin 18.2 expression is restricted to the stomach.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Transfected cell lysate - Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-Claudin18 antibody [43-14A] - BSA and Azide free (ab314691) is a mouse recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Other related products
We have a range of other formats of antibody clone [43-14A] also available for your convenience: ab314690, Carrier free - ab314691

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin-18 often noted as Claudin18 or CLDN18 is a tight junction protein belonging to the Claudin family. The protein comes in different isoforms including Claudin 18.2 each having a distinct function and expression pattern. With an estimated molecular mass of approximately 24 kDa it is mainly expressed in epithelial cells of the stomach and specific lung epithelia. Researchers utilize it frequently in immunohistochemistry (IHC) studies often referred to as CLDN18 IHC for its role in maintaining cell-to-cell adhesion in these tissues.
Biological function summary

Claudin-18.2 integrates itself into a tight junction complex contributing to the selective permeability barrier in epithelial tissues. This barrier is essential for maintaining tissue homeostasis and protecting underlying tissue from various external factors. It plays a significant role in ion selectivity and barrier assurance of the epithelial cells-gastrointestinal site especially. The regulated expression and function of Claudin 18 are critical for effective epithelial barrier function.

Pathways

Claudin-18 is involved in pathways concerning epithelial morphogenesis and integrity. It aligns with occludins and other claudins to control paracellular transport and cellular integrity within these pathways. Through its association with proteins such as ZO-1 Claudin-18 ensures proper cell signaling and tight junction formation. Its regulation in epithelial cells contributes significantly to pathways that sustain cell structure and prevent uncontrolled leakage of solutes.

The altered expression of Claudin-18.2 is closely linked to gastric and lung cancers. Abnormal levels can disrupt the epithelial barrier contributing to tumor progression and metastasis. In gastric cancer altered Claudin-18 couples with overexpression of other proteins such as HER2 playing a role in the disease's aggressiveness. Researchers consider it a potential target for therapeutic interventions making Claudin-18.2 purchase or acquisition relevant for studies oriented toward cancer diagnostics and treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in alveolar fluid homeostasis via regulation of alveolar epithelial tight junction composition and therefore ion transport and solute permeability, potentially via downstream regulation of the actin cytoskeleton organization and beta-2-adrenergic signaling (By similarity). Required for lung alveolarization and maintenance of the paracellular alveolar epithelial barrier (By similarity). Acts to maintain epithelial progenitor cell proliferation and organ size, via regulation of YAP1 localization away from the nucleus and thereby restriction of YAP1 target gene transcription (By similarity). Acts as a negative regulator of RANKL-induced osteoclast differentiation, potentially via relocation of TJP2/ZO-2 away from the nucleus, subsequently involved in bone resorption in response to calcium deficiency (By similarity). Mediates the osteoprotective effects of estrogen, potentially via acting downstream of estrogen signaling independently of RANKL signaling pathways (By similarity).. Isoform A1. Involved in the maintenance of homeostasis of the alveolar microenvironment via regulation of pH and subsequent T-cell activation in the alveolar space, is therefore indirectly involved in limiting C.neoformans infection.. Isoform A2. Required for the formation of the gastric paracellular barrier via its role in tight junction formation, thereby involved in the response to gastric acidification.
See full target information CLDN18
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of the American Association for Cancer Research 14:7624-34 PubMed19047087

2008

Claudin-18 splice variant 2 is a pan-cancer target suitable for therapeutic antibody development.

Applications

Unspecified application

Species

Unspecified reactive species

Ugur Sahin,Michael Koslowski,Karl Dhaene,Dirk Usener,Gunda Brandenburg,Gerhard Seitz,Christoph Huber,Ozlem Türeci

Clinical cancer research : an official journal of the American Association for Cancer Research 14:7635-44 PubMed19047088

2008

Perilobar nephrogenic rests are nonobligate molecular genetic precursor lesions of insulin-like growth factor-II-associated Wilms tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Raisa Vuononvirta,Neil J Sebire,Anthony R Dallosso,Jorge S Reis-Filho,Richard D Williams,Alan Mackay,Kerry Fenwick,Anita Grigoriadis,Alan Ashworth,Kathy Pritchard-Jones,Keith W Brown,Gordan M Vujanic,Chris Jones
View all publications
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Product promise

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