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Anti-Claudin18 antibody [43-14A] - BSA and Azide free (ab314691) is a mouse recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Claudin18 in IHC-P. Suitable for Human.



- BSA, sodium azide, and glycerol-free for easy conjugation

- Biophysical QC for unrivalled batch-batch consistency


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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (AB314691), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (AB314691), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (AB314691), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (AB314691), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Transfected cell lysate - Human
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Target data

Function

The protein expressed by the gene CLDN18 plays a major role in the tight junction-specific obliteration of the intercellular space through calcium-independent cell-adhesion activity. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

UNQ778/PRO1572, CLDN18, Claudin-18

Recommended products

Anti-Claudin18 antibody [43-14A] - BSA and Azide free (ab314691) is a mouse recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Claudin18 in IHC-P. Suitable for Human.



- BSA, sodium azide, and glycerol-free for easy conjugation

- Biophysical QC for unrivalled batch-batch consistency


Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
43-14A
Purification technique
Affinity purification Protein A
Specificity

Cross-reactivity has been checked and this antibody might cross with Claudin18.1 and Claudin18.2.
The human gene CLDN18 has two protein isoforms, Claudin 18.1 and Claudin 18.2. The two isoforms differ within the N-terminal 69 amino acids. Claudin 18.1 expression is restricted to the lung. In contrast Claudin 18.2 expression is restricted to the stomach.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

What is this antibody validated in?


Anti-Claudin18 antibody [43-14A] - BSA and Azide free (ab314691) is a mouse recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Other related products


We have a range of other formats of antibody clone [43-14A] also available for your convenience:
Anti-Claudin18 antibody [43-14A] ab314690, Carrier free - ab314691



Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Claudin-18 often noted as Claudin18 or CLDN18 is a tight junction protein belonging to the Claudin family. The protein comes in different isoforms including Claudin 18.2 each having a distinct function and expression pattern. With an estimated molecular mass of approximately 24 kDa it is mainly expressed in epithelial cells of the stomach and specific lung epithelia. Researchers utilize it frequently in immunohistochemistry (IHC) studies often referred to as CLDN18 IHC for its role in maintaining cell-to-cell adhesion in these tissues.

Biological function summary

Claudin-18.2 integrates itself into a tight junction complex contributing to the selective permeability barrier in epithelial tissues. This barrier is essential for maintaining tissue homeostasis and protecting underlying tissue from various external factors. It plays a significant role in ion selectivity and barrier assurance of the epithelial cells-gastrointestinal site especially. The regulated expression and function of Claudin 18 are critical for effective epithelial barrier function.

Pathways

Claudin-18 is involved in pathways concerning epithelial morphogenesis and integrity. It aligns with occludins and other claudins to control paracellular transport and cellular integrity within these pathways. Through its association with proteins such as ZO-1 Claudin-18 ensures proper cell signaling and tight junction formation. Its regulation in epithelial cells contributes significantly to pathways that sustain cell structure and prevent uncontrolled leakage of solutes.

Associated diseases and disorders

The altered expression of Claudin-18.2 is closely linked to gastric and lung cancers. Abnormal levels can disrupt the epithelial barrier contributing to tumor progression and metastasis. In gastric cancer altered Claudin-18 couples with overexpression of other proteins such as HER2 playing a role in the disease's aggressiveness. Researchers consider it a potential target for therapeutic interventions making Claudin-18.2 purchase or acquisition relevant for studies oriented toward cancer diagnostics and treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (ab314691), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (ab314691)

    This data was developed using Anti-Claudin18 antibody [43-14A] ab314690, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Claudin18.2 expression vector containing a His tag. (B) HEK-293T transfected with a Claudin18.1 expression vector containing a His tag. (C) HEK-293T transfected with empty vector containing a His tag. tissue labeling Claudin18 with Anti-Claudin18 antibody [43-14A] ab314690 at 1/10000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on (A) HEK-293T transfected with a Claudin18.2 expression vector containing a His tag and (B) HEK-293T transfected with a Claudin18.1 expression vector containing a His tag. No staining on (C) HEK-293T transfected with empty vector containing a His tag. The section was incubated with Anti-Claudin18 antibody [43-14A] ab314690 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (ab314691), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (ab314691)

    This data was developed using Anti-Claudin18 antibody [43-14A] ab314690, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Claudin18 with Anti-Claudin18 antibody [43-14A] ab314690 at 1/5000 (0.2096 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Negative control: no staining on human pancreas. The section was incubated with Anti-Claudin18 antibody [43-14A] ab314690 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (ab314691), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (ab314691)

    This data was developed using Anti-Claudin18 antibody [43-14A] ab314690, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling Claudin18 with Anti-Claudin18 antibody [43-14A] ab314690 at 1/5000 (0.2096 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on human gastric adenocarcinoma. The section was incubated with Anti-Claudin18 antibody [43-14A] ab314690 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (ab314691), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [43-14A] - BSA and Azide free (ab314691)

    This data was developed using Anti-Claudin18 antibody [43-14A] ab314690, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Claudin18 with Anti-Claudin18 antibody [43-14A] ab314690 at 1/5000 (0.2096 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on human stomach. The section was incubated with Anti-Claudin18 antibody [43-14A] ab314690 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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