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AB222513

Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

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(1 Publication)

Rabbit Recombinant Monoclonal Claudin 18 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

UNQ778/PRO1572, CLDN18, Claudin-18

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (AB222513)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (AB222513)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Claudin18.2 + Claudin18.1 with ab222513 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human lung epithelial cells (PMID : 21571049). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Antigen retrieval : Heat mediated antigen retrieval performed using pH 9 buffer.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (AB222513)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (AB222513)

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue labeling Claudin18.2 + Claudin18.1 with ab222513 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human pancreas cancer cells (PMID : 21832145). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Antigen retrieval : Heat mediated antigen retrieval performed using citrate buffer pH 6.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (AB222513)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (AB222513)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Claudin18.2 + Claudin18.1 with ab222513 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human gastric epithelial cells (PMID : 21571049). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Antigen retrieval : Heat mediated antigen retrieval performed using citrate buffer pH 6.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (AB222513)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (AB222513)

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling Claudin18.2 + Claudin18.1 with ab222513 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Antigen retrieval : Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on human gastric cancer. The section was incubated with ab222513 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Carrier free

    Anti-Claudin18.2+Claudin18.1 antibody [EPR19203] - BSA and Azide free

  • 578 PE

    PE Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 660 APC

    APC Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19203

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin18.2 and Claudin18.1 are members of the tight junction protein family known for their role in maintaining cell-cell adhesion and sealing epithelial barriers. These proteins often referred to simply as Claudin18 have a molecular mass of approximately 26 kDa. Claudin18.2 expresses prominently in the stomach lining while Claudin18.1 is found in lung tissue. Both variants localize to tight junctions underlining their importance in forming selective cellular barriers that regulate paracellular transport.
Biological function summary

Claudin18.2 and Claudin18.1 are essential components in maintaining epithelial integrity. Claudin proteins form the backbone of tight junction strands contributing to the segregation and compartmentalization within tissues. These proteins interact with other tight junction proteins like Occludin and Zonula Occludens to form a complex which regulates permeability and protects tissues from pathogens and toxins. Their tight sealing property ensures that epithelial tissues control the passage of molecules between cells maintaining homeostasis and protecting underlying tissues from external environments.

Pathways

Claudin18.2 and Claudin18.1 integrate into the tight junction signaling pathway which is important for cell polarity and barrier function. The tight junction signaling pathway coordinates with other pathways such as the Epithelial-Mesenchymal Transition (EMT) pathway which is important in development and wound healing. Proteins like E-cadherin influence this pathway by balancing adhesion and mobility where Claudin18 proteins act to stabilize the junctions therefore controlling epithelial tissue dynamics.

Alterations in Claudin18.2 and Claudin18.1 expression associate with gastric cancer and asthma respectively. In gastric cancer overexpression of Claudin18.2 can contribute to tumor growth by facilitating cellular disorganization. It interacts with proteins like HER2 in cancer pathways suggesting a role in cell proliferation. For asthma Claudin18.1 might influence lung epithelial permeability and facilitate inflammatory processes suggesting it works with cytokines like IL-4 in the inflammatory response. Understanding these connections helps in developing targeted therapies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the gene CLDN18 plays a major role in the tight junction-specific obliteration of the intercellular space through calcium-independent cell-adhesion activity. This supplementary information is collated from multiple sources and compiled automatically.
See full target information CLDN18
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1636367 PubMed40951359

2025

Claudin18.2 defines a prognostically distinct subgroup of intrahepatic cholangiocarcinoma via CD8 T-cell exclusion.

Applications

Unspecified application

Species

Unspecified reactive species

Chengui Yu,Yuren Pan,Fuli Li,Zhenyun Guo,Da Xu,Ying Zhu,Baobing Yin
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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