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Rabbit Recombinant Monoclonal Claudin 18 antibody. Suitable for IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (AB222513), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (AB222513), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (AB222513), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (AB222513), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Target data

Function

The protein expressed by the gene CLDN18 plays a major role in the tight junction-specific obliteration of the intercellular space through calcium-independent cell-adhesion activity. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

UNQ778/PRO1572, CLDN18, Claudin-18

Recommended products

Rabbit Recombinant Monoclonal Claudin 18 antibody. Suitable for IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19203
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Claudin18.2 and Claudin18.1 are members of the tight junction protein family known for their role in maintaining cell-cell adhesion and sealing epithelial barriers. These proteins often referred to simply as Claudin18 have a molecular mass of approximately 26 kDa. Claudin18.2 expresses prominently in the stomach lining while Claudin18.1 is found in lung tissue. Both variants localize to tight junctions underlining their importance in forming selective cellular barriers that regulate paracellular transport.

Biological function summary

Claudin18.2 and Claudin18.1 are essential components in maintaining epithelial integrity. Claudin proteins form the backbone of tight junction strands contributing to the segregation and compartmentalization within tissues. These proteins interact with other tight junction proteins like Occludin and Zonula Occludens to form a complex which regulates permeability and protects tissues from pathogens and toxins. Their tight sealing property ensures that epithelial tissues control the passage of molecules between cells maintaining homeostasis and protecting underlying tissues from external environments.

Pathways

Claudin18.2 and Claudin18.1 integrate into the tight junction signaling pathway which is important for cell polarity and barrier function. The tight junction signaling pathway coordinates with other pathways such as the Epithelial-Mesenchymal Transition (EMT) pathway which is important in development and wound healing. Proteins like E-cadherin influence this pathway by balancing adhesion and mobility where Claudin18 proteins act to stabilize the junctions therefore controlling epithelial tissue dynamics.

Associated diseases and disorders

Alterations in Claudin18.2 and Claudin18.1 expression associate with gastric cancer and asthma respectively. In gastric cancer overexpression of Claudin18.2 can contribute to tumor growth by facilitating cellular disorganization. It interacts with proteins like HER2 in cancer pathways suggesting a role in cell proliferation. For asthma Claudin18.1 might influence lung epithelial permeability and facilitate inflammatory processes suggesting it works with cytokines like IL-4 in the inflammatory response. Understanding these connections helps in developing targeted therapies for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (ab222513), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (ab222513)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Claudin18.2 + Claudin18.1 with ab222513 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human gastric epithelial cells (PMID: 21571049). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Antigen retrieval: Heat mediated antigen retrieval performed using citrate buffer, pH 6.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (ab222513), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (ab222513)

    Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Claudin18.2 + Claudin18.1 with ab222513 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human lung epithelial cells (PMID: 21571049). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Antigen retrieval: Heat mediated antigen retrieval performed using pH 9 buffer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (ab222513), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (ab222513)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue labeling Claudin18.2 + Claudin18.1 with ab222513 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human pancreas cancer cells (PMID: 21832145). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Antigen retrieval: Heat mediated antigen retrieval performed using citrate buffer, pH 6.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (ab222513), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203] (ab222513)

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling Claudin18.2 + Claudin18.1 with ab222513 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) Ready to use. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) Ready to use.
    Antigen retrieval: Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
    Positive staining on human gastric cancer. The section was incubated with ab222513 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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