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AB251570

Anti-Claudin18.2+Claudin18.1 antibody [EPR19203] - BSA and Azide free

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Rabbit Recombinant Monoclonal Claudin 18 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

UNQ778/PRO1572, CLDN18, Claudin-18

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2+Claudin18.1 antibody [EPR19203] - BSA and Azide free (AB251570)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2+Claudin18.1 antibody [EPR19203] - BSA and Azide free (AB251570)

This data was developed using ab222513, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Claudin18.2 + Claudin18.1 with ab222513 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human lung epithelial cells (PMID : 21571049). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Antigen retrieval : Heat mediated antigen retrieval performed using pH 9 buffer.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2+Claudin18.1 antibody [EPR19203] - BSA and Azide free (AB251570)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2+Claudin18.1 antibody [EPR19203] - BSA and Azide free (AB251570)

This data was developed using ab222513, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue labeling Claudin18.2 + Claudin18.1 with ab222513 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human pancreas cancer cells (PMID : 21832145). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Antigen retrieval : Heat mediated antigen retrieval performed using citrate buffer, pH 6.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2+Claudin18.1 antibody [EPR19203] - BSA and Azide free (AB251570)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2+Claudin18.1 antibody [EPR19203] - BSA and Azide free (AB251570)

This data was developed using ab222513, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Claudin18.2 + Claudin18.1 with ab222513 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human gastric epithelial cells (PMID : 21571049). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Antigen retrieval : Heat mediated antigen retrieval performed using citrate buffer, pH 6.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2+Claudin18.1 antibody [EPR19203] - BSA and Azide free (AB251570)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2+Claudin18.1 antibody [EPR19203] - BSA and Azide free (AB251570)

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling Claudin18.2 + Claudin18.1 with ab222513 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Antigen retrieval : Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on human gastric cancer. The section was incubated with ab222513 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222513).

  • Unconjugated

    Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 660 APC

    APC Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 578 PE

    PE Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19203

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab251570 is the carrier-free version of ab222513.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin18.2 and Claudin18.1 are integral membrane proteins part of the claudin family which are tight junction proteins. Claudin18.2 has a molecular weight of approximately 27 kDa. These proteins are primarily expressed in epithelial cells particularly within the gastric mucosa and lung tissue. They serve as essential components of cell-cell adhesion influencing paracellular transport and maintaining cell polarity.
Biological function summary

These proteins are vital in forming tight junction strands contributing to cellular barrier functions. Claudin18.2 and Claudin18.1 belong to a multi-protein complex vital for establishing cell permeability limits. Through their integration in tight junctions they regulate ion passage minimize fluid loss and maintain tissue homeostasis. Their dynamic nature allows adaptation in response to physiological stimuli making them central to epithelial barrier integrity.

Pathways

Claudins including Claudin18.2 and Claudin18.1 play roles in signaling pathways like Wnt and MAPK which are pivotal for cellular processes like proliferation and differentiation. These pathways involve interactions with proteins like ZO-1 and occludin contributing to the signaling processes across the epithelial cells. This involvement with important signaling proteins highlights their importance in maintaining cell function and signaling responses.

These claudins have strong associations with gastric cancer and asthma pathophysiology. Overexpression of Claudin18.2 has been shown in gastric cancer indicating its potential as a therapeutic target. In asthma altered expression of Claudin18.1 affects lung epithelial integrity linking these proteins to airway inflammation and remodeling. The connection with proteins like E-cadherin and beta-catenin further highlights their role in tumor progression and epithelial transformation making them relevant in clinical research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in alveolar fluid homeostasis via regulation of alveolar epithelial tight junction composition and therefore ion transport and solute permeability, potentially via downstream regulation of the actin cytoskeleton organization and beta-2-adrenergic signaling (By similarity). Required for lung alveolarization and maintenance of the paracellular alveolar epithelial barrier (By similarity). Acts to maintain epithelial progenitor cell proliferation and organ size, via regulation of YAP1 localization away from the nucleus and thereby restriction of YAP1 target gene transcription (By similarity). Acts as a negative regulator of RANKL-induced osteoclast differentiation, potentially via relocation of TJP2/ZO-2 away from the nucleus, subsequently involved in bone resorption in response to calcium deficiency (By similarity). Mediates the osteoprotective effects of estrogen, potentially via acting downstream of estrogen signaling independently of RANKL signaling pathways (By similarity).. Isoform A1. Involved in the maintenance of homeostasis of the alveolar microenvironment via regulation of pH and subsequent T-cell activation in the alveolar space, is therefore indirectly involved in limiting C.neoformans infection.. Isoform A2. Required for the formation of the gastric paracellular barrier via its role in tight junction formation, thereby involved in the response to gastric acidification.
See full target information CLDN18
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Product promise

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