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AB249106

Anti-ClC-2 antibody [EPR6492(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ClC-2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Chloride channel protein 2, ClC-2, CLCN2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ClC-2 antibody [EPR6492(2)] - BSA and Azide free (AB249106)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ClC-2 antibody [EPR6492(2)] - BSA and Azide free (AB249106)

This data was developed using ab154798, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling ClC-2 with ab154798 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-ClC-2 antibody [EPR6492(2)] - BSA and Azide free (AB249106)
  • WB

Unknown

Western blot - Anti-ClC-2 antibody [EPR6492(2)] - BSA and Azide free (AB249106)

This data was developed using ab154798, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ClC-2 antibody [EPR6492(2)] (<a href='/en-us/products/primary-antibodies/clc-2-antibody-epr64922-ab154798'>ab154798</a>) at 1/1000 dilution

Lane 1:

HT29 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Predicted band size: 99 kDa

false

Western blot - Anti-ClC-2 antibody [EPR6492(2)] - BSA and Azide free (AB249106)
  • WB

Unknown

Western blot - Anti-ClC-2 antibody [EPR6492(2)] - BSA and Azide free (AB249106)

This data was developed using ab154798, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ClC-2 antibody [EPR6492(2)] (<a href='/en-us/products/primary-antibodies/clc-2-antibody-epr64922-ab154798'>ab154798</a>) at 1/1000 dilution

Lane 1:

mouse brain lysate at 10 µg

Lane 2:

mouse heart lysate at 10 µg

Lane 3:

mouse kidney lysate at 10 µg

Lane 4:

mouse spleen lysate at 10 µg

Lane 5:

rat kidney lysate at 10 µg

Lane 6:

rat spleen lysate at 10 µg

Predicted band size: 99 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6492(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab249106 is the carrier-free version of ab154798.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ClC-2 or chloride channel 2 is a voltage-gated chloride channel found in the cell membranes. It belongs to the CLC family of chloride channels and transporters weighing approximately 97 kDa. The expression of ClC-2 can be seen in various tissues throughout the body with high levels observed in the brain and intestinal epithelial cells. ClC-2 functions mechanically by facilitating the passive flow of chloride ions across the cell membrane therefore influencing the membrane potential and cellular excitability.
Biological function summary

Chloride channel 2 plays an important role in maintaining cell volume and electrical stability. By regulating the flow of ions ClC-2 supports processes such as signal transmission in neurons and hydration in the bowel. While not directly part of a larger complex its function is influenced by interactions with other membrane proteins which help modulate its activity under different physiological conditions.

Pathways

Chloride channel 2 is involved in cellular processes including the epithelial transport of ions and neuronal signaling pathways. Within the gastrointestinal tract ClC-2 participates in the signaling pathway that maintains fluid balance and absorption. It also interacts with proteins like CFTR to ensure proper ion transport which is particularly important under conditions that stimulate chloride secretion.

Chloride channel 2 has been associated with conditions such as epilepsy and chloride diarrhea. Mutations or dysregulation of ClC-2 can lead to defective ion homeostasis. In epilepsy abnormal ClC-2 function may contribute to neuronal hyperexcitability. Furthermore the relationship between ClC-2 and CFTR is implicated in chloride diarrhea where impaired chloride transport affects intestinal fluid secretion.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Voltage-gated and osmosensitive chloride channel. Forms a homodimeric channel where each subunit has its own ion conduction pathway. Conducts double-barreled currents controlled by two types of gates, two fast glutamate gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously. Displays inward rectification currents activated upon membrane hyperpolarization and extracellular hypotonicity (PubMed : 16155254, PubMed : 17567819, PubMed : 19191339, PubMed : 23632988, PubMed : 29403011, PubMed : 29403012, PubMed : 36964785, PubMed : 38345841). Contributes to chloride conductance involved in neuron excitability. In hippocampal neurons, generates a significant part of resting membrane conductance and provides an additional chloride efflux pathway to prevent chloride accumulation in dendrites upon GABA receptor activation. In glia, associates with the auxiliary subunit HEPACAM/GlialCAM at astrocytic processes and myelinated fiber tracts where it may regulate transcellular chloride flux buffering extracellular chloride and potassium concentrations (PubMed : 19191339, PubMed : 22405205, PubMed : 23707145). Regulates aldosterone production in adrenal glands. The opening of CLCN2 channels at hyperpolarized membrane potentials in the glomerulosa causes cell membrane depolarization, activation of voltage-gated calcium channels and increased expression of aldosterone synthase, the rate-limiting enzyme for aldosterone biosynthesis (PubMed : 29403011, PubMed : 29403012). Contributes to chloride conductance in retinal pigment epithelium involved in phagocytosis of shed photoreceptor outer segments and photoreceptor renewal (PubMed : 36964785). Conducts chloride currents at the basolateral membrane of epithelial cells with a role in chloride reabsorption rather than secretion (By similarity) (PubMed : 16155254). Permeable to small monovalent anions with chloride > thiocyanate > bromide > nitrate > iodide ion selectivity (By similarity) (PubMed : 29403012).
See full target information CLCN2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze
View all publications
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Product promise

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