Anti-CLCA1 antibody [EPR12254-88]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (AB180851)

Immunofluorescence analysis of A431 cells labeling CLCA1 using unpurified ab180851 at 1/50 dilution.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CLCA1 antibody [EPR12254-88] (AB180851)

Intracellular Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling CLCA1 with purified ab180851 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (AB180851)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human small intestine tissue sections labeling CLCA1 with Purified ab180851 at 1 : 4000 dilution (0.07 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (AB180851)

Immunocytochemistry/ Immunofluorescence analysis of SW480 (Human colorectal adenocarcinoma epithelial cell) cells labeling CLCA1 with Purified ab180851 at 1 : 100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (AB180851)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CLCA1 using unpurified ab180851 at 1/100 dilution.

• WB

Supplier Data

Western blot - Anti-CLCA1 antibody [EPR12254-88] (AB180851)

All lanes:

Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/1000 dilution

Lane 1:

Human colon cancer lysates at 10 µg

Lane 2:

Human small intestine lysates at 10 µg

Predicted band size: 100 kDa

false

• WB

Lab

Western blot - Anti-CLCA1 antibody [EPR12254-88] (AB180851)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/10000 dilution

All lanes:

Human small intestine lysates at 15 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 100 kDa

false

• WB

Lab

Western blot - Anti-CLCA1 antibody [EPR12254-88] (AB180851)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/50000 dilution

Lane 1:

Rat small intestine lysates at 15 µg

Lane 2:

Mouse small intestine lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 100 kDa

false