Rabbit Recombinant Monoclonal CLCA1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples.
Images
![Western blot - Anti-CLCA1 antibody [EPR12254] (AB174319), expandable thumbnail](https://content.abcam.com/products/images/clca1-antibody-epr12254-ab174319--western-blot-img147551.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CLCA1 antibody [EPR12254] (AB174319)")
![Immunoprecipitation - Anti-CLCA1 antibody [EPR12254] (AB174319), expandable thumbnail](https://content.abcam.com/products/images/clca1-antibody-epr12254-ab174319--immunoprecipitation-img53460.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CLCA1 antibody [EPR12254] (AB174319)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254] (AB174319), expandable thumbnail](https://content.abcam.com/products/images/clca1-antibody-epr12254-ab174319--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34189.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254] (AB174319)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC.
Alternative names
CACC1, CLCA1, Calcium-activated chloride channel regulator 1, Calcium-activated chloride channel family member 1, Calcium-activated chloride channel protein 1, hCLCA1, CaCC-1, hCaCC-1
Recommended products
Rabbit Recombinant Monoclonal CLCA1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12254
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CLCA1 antibody [EPR12254] (ab174319), expandable thumbnail](https://content.abcam.com/products/images/clca1-antibody-epr12254-ab174319--western-blot-img147551.jpg "Western blot - Anti-CLCA1 antibody [EPR12254] (ab174319)")
Western blot - Anti-CLCA1 antibody [EPR12254] (ab174319)
All lanes: Western blot - Anti-CLCA1 antibody [EPR12254] (ab174319) at 1/10000 dilution
Lane 1: Human colon cancer lysate at 10 µg
Lane 2: Human small intestine lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 100 kDa
![Immunoprecipitation - Anti-CLCA1 antibody [EPR12254] (ab174319), expandable thumbnail](https://content.abcam.com/products/images/clca1-antibody-epr12254-ab174319--immunoprecipitation-img53460.jpg "Immunoprecipitation - Anti-CLCA1 antibody [EPR12254] (ab174319)")
Immunoprecipitation - Anti-CLCA1 antibody [EPR12254] (ab174319)
Western blot analysis on immunoprecipitation pellet from Human colon cancer lysate using ab174319 at 1/10 dilution.
All lanes: Immunoprecipitation - Anti-CLCA1 antibody [EPR12254] (ab174319)
Predicted band size: 100 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254] (ab174319), expandable thumbnail](https://content.abcam.com/products/images/clca1-antibody-epr12254-ab174319--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34189.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254] (ab174319)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254] (ab174319)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CLCA1 with ab174319 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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