Anti-CLCA4 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA4 antibody (AB197347)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue sections labeling CLCA4 with ab197347 at 1/10 dilution.

• WB

Supplier Data

Western blot - Anti-CLCA4 antibody (AB197347)

Gel : 8%SDS-PAGE.

All lanes:

Western blot - Anti-CLCA4 antibody (ab197347) at 1/200 dilution

All lanes:

293T cell lysate at 40 µg

Secondary

All lanes:

Anti-rabbit IgG at 1/8000 dilution

Predicted band size: 101 kDa

false

Exposure time: 30s

• WB

CiteAb

Western blot - Anti-CLCA4 antibody (AB197347)

CLCA4 western blot using anti-CLCA4 antibody ab197347. Publication image and figure legend from Liu, Z., Chen, M., et al., 2018, Aging (Albany NY), PubMed 30312171.

ab197347 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab197347 please see the product overview.

CLCA4expression was correlated with the expression of epithelial-mesenchymal transition (EMT) markers. (A) Serial sections of human HCC tissue were subjected to IHC staining with antibodies against CLCA4, E-cadherin, Vimentin and N-cadherin. In patient #1, low CLCA4 expression in HCC tissues was accompanied by the absence of E-cadherin and elevated Vimentin, N-cadherin. In patient #2, high CLCA4 expression was accompanied by elevated E-cadherin and the absence of Vimentin, N-cadherin. The scale bar represents 50 μm. (B) CLCA4 expression was positively associated with E-cadherin expression and negatively correlated with vimentin and N-cadherin expression. (C) Decreased expression of E-cadherin with increased expression of Vimentin and N-cadherin in CLCA4-silenced cells compared with the control cells. An opposite expression pattern of these genes was observed in CLCA4-transfected cells. (D) The mRNA of E-cadherin was down-regulated, while the vimentin and N-cadherin was up-regulated, when CLCA4 was silenced. And opposite expression was observed in CLCA4-transfected cells.

false