Anti-CLCC1 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CLCC1 antibody (AB237755)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for CLCC1 (green) using ab237755 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488 conjugated Goat Anti-Rabbit IgG (H+L).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCC1 antibody (AB237755)

Paraffin-embedded human liver cancer tissue stained for CLCC1 using ab237755 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCC1 antibody (AB237755)

Paraffin-embedded human testis tissue stained for CLCC1 using ab237755 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

• IP

Supplier Data

Immunoprecipitation - Anti-CLCC1 antibody (AB237755)

CLCC1 was immunoprecipitated from 500 μg HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab237755 at 1/200 dilution.

Lane 1 : Rabbit control IgG IP in HepG2 whole cell lysate.

Lane 2 : ab237751 IP in HepG2 whole cell lysate.

Lane 3 : HepG2 whole cell lysate 20 μg (Input).

For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

All lanes:

Immunoprecipitation - Anti-CLCC1 antibody (ab237755)

Predicted band size: 62 kDa

false

• WB

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Western blot - Anti-CLCC1 antibody (AB237755)

All lanes:

Western blot - Anti-CLCC1 antibody (ab237755) at 1/500 dilution

Lane 1:

COLO320 whole cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) whole cell lysate

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 4:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 5:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 62 kDa

false