AB189857

Anti-CLCN1 antibody - C-terminal

Name: Anti-CLCN1 antibody - C-terminal (ab189857) | Abcam
Brand: Abcam Limited
SKU: AB189857

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(10 Publications)

Rabbit Polyclonal CLCN1 antibody. C-terminal. Suitable for WB and reacts with Human, Mouse samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CLCN1.

View Alternative Names

CLC1, CLCN1, Chloride channel protein 1, ClC-1

2 Images

Supplier Data

Western blot - Anti-CLCN1 antibody - C-terminal (AB189857)

Blocking buffer : 3% nonfat dry milk in TBST.

Detection : ECL Enhanced Kit.

All lanes:

Western blot - Anti-CLCN1 antibody - C-terminal (ab189857) at 1/1000 dilution

All lanes:

Mouse skeletal muscle extracts at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 109 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-CLCN1 antibody - C-terminal (AB189857)

All lanes:

Western blot - Anti-CLCN1 antibody - C-terminal (ab189857) at 1/500 dilution

Lane 1:

293T cell extract

Lane 2:

Mouse liver tissue extract

Predicted band size: 109 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human CLCN1. The exact immunogen used to generate this antibody is proprietary information.

P35523

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Voltage-gated chloride channel involved in skeletal muscle excitability. Generates most of the plasma membrane chloride conductance in skeletal muscle fibers, stabilizes the resting membrane potential and contributes to the repolarization phase during action potential firing (PubMed : 12456816, PubMed : 16027167, PubMed : 22521272, PubMed : 22641783, PubMed : 26007199, PubMed : 26502825, PubMed : 26510092, PubMed : 7951242, PubMed : 8112288, PubMed : 8130334, PubMed : 9122265, PubMed : 9565403, PubMed : 9736777). Forms a homodimeric channel where each subunit has its own ion conduction pathway. Conducts double-barreled currents controlled by two types of gates, two fast glutamate gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously. Has a significant open probability at muscle resting potential and is further activated upon membrane depolarization (PubMed : 10051520, PubMed : 10962018, PubMed : 29809153, PubMed : 31022181). Permeable to small monovalent anions with ion selectivity for chloride > thiocyanate > bromide > nitrate > iodide (PubMed : 9122265, PubMed : 9565403).

See full target information CLCN1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 15:1445660 PubMed40171164

2025

Divergent host humoral innate immune response to the smooth-to-rough adaptation of in chronic infection.

Applications

Unspecified application

Species

Unspecified reactive species

Emily A Wheeler,Patricia M Lenhart-Pendergrass,Noel M Rysavy,Katie R Poch,Silvia M Caceres,Kara M Calhoun,Karina A Serban,Jerry A Nick,Kenneth C Malcolm

Frontiers in genetics 15:1486977 PubMed39712484

2024

Case report: Multiple approach analysis in a case of clinically assessed myotonia congenita.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Lucchiari,Francesco Fortunato,Giovanni Meola,Andrea Mignarri,Serena Pagliarani,Stefania Corti,Giacomo P Comi,Dario Ronchi

Physiological reports 12:e70081 PubMed39523499

2024

Associations between skeletal muscle phenotype, positional role, and on-ice performance in elite male ice hockey players.

Applications

Unspecified application

Species

Unspecified reactive species

Jeppe F Vigh-Larsen,Hallur Thorsteinsson,Martin Thomassen,Jeppe Panduro,Bjørn Fristrup,Morten B Randers,Jens L Olesen,Peter Krustrup,Kristian Overgaard,Lars Nybo,Magni Mohr

Journal of muscle research and cell motility 44:25-36 PubMed37014477

2023

Six weeks of high-load resistance and low-load blood flow restricted training increase Na/K-ATPase sub-units α2 and β1 equally, but does not alter ClC-1 abundance in untrained human skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Jakob Wang,Emil Rindom,Thomas Groennebaek,Peter Sieljacks,Jesper Emil Jakobsgaard,Jean Farup,Kristian Vissing,Thomas Holm Pedersen,Frank Vincenzo de Paoli

International journal of molecular sciences 24: PubMed36835205

2023

Vorinostat Improves Myotonic Dystrophy Type 1 Splicing Abnormalities in DM1 Muscle Cell Lines and Skeletal Muscle from a DM1 Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Nafisa Neault,Aymeric Ravel-Chapuis,Stephen D Baird,John A Lunde,Mathieu Poirier,Emiliyan Staykov,Julio Plaza-Diaz,Gerardo Medina,Francisco Abadía-Molina,Bernard J Jasmin,Alex E MacKenzie

American journal of physiology. Cell physiology 322:C395-C409 PubMed35080921

2022

Chloride channels with ClC-1-like properties differentially regulate the excitability of dopamine receptor D1- and D2-expressing striatal medium spiny neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Viktor Yarotskyy,Arianna R S Lark,Sara R Nass,Yun K Hahn,Michael G Marone,A Rory McQuiston,Pamela E Knapp,Kurt F Hauser

Scandinavian journal of medicine & science in sports 32 Suppl 1:39-53 PubMed34427373

2021

Skeletal muscle phenotype and game performance in elite women football players.

Applications

Unspecified application

Species

Unspecified reactive species

Magni Mohr,Ioannis G Fatouros,Athanasios Z Jamurtas,Dimitrios Draganidis,Martin Thomassen,Christina Ørntoft,Georgios Ermidis,Georgios Loules,Dimitrios Batsilas,Athanasios Poulios,Konstantinos Papanikolaou,Morten B Randers,Peter Krustrup,Lars Nybo

Frontiers in neurology 11:1019 PubMed33013670

2020

Pathomechanisms of a Mutation Found in a Russian Family Suffering From Becker's Myotonia.

Applications

Unspecified application

Species

Unspecified reactive species

Concetta Altamura,Evgeniya A Ivanova,Paola Imbrici,Elena Conte,Giulia Maria Camerino,Elena L Dadali,Alexander V Polyakov,Sergei Aleksandrovich Kurbatov,Francesco Girolamo,Maria Rosaria Carratù,Jean-François Desaphy

Cell death & disease 11:149 PubMed32094348

2020

Suppressing STAT5 signaling affects osteosarcoma growth and stemness.

Applications

Unspecified application

Species

Unspecified reactive species

Dharmalingam Subramaniam,Pablo Angulo,Sivapriya Ponnurangam,Prasad Dandawate,Prabhu Ramamoorthy,Pugazhendhi Srinivasan,Tomoo Iwakuma,Scott J Weir,Katherine Chastain,Shrikant Anant

Journal of applied physiology (Bethesda, Md. : 198 125:470-478 PubMed29722626

2018

Abundance of ClC-1 chloride channel in human skeletal muscle: fiber type specific differences and effect of training.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Thomassen,Morten Hostrup,Robyn M Murphy,Brett A Cromer,Casper Skovgaard,Thomas P Gunnarsson,Peter M Christensen,Jens Bangsbo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CLCN1 antibody - C-terminal

Western blot - Anti-CLCN1 antibody - C-terminal (AB189857)

Western blot - Anti-CLCN1 antibody - C-terminal (AB189857)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (10)

Product promise