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Rabbit Recombinant Monoclonal GSDMD antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] - BSA and Azide free (AB255984), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Gasdermin-DPrecursor of a pore-forming protein that plays a key role in host defense against pathogen infection and danger signals (PubMed:26375003, PubMed:26375259, PubMed:27281216). This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-D, N-terminal) binds to membranes and forms pores, triggering pyroptosis (PubMed:26375003, PubMed:26375259, PubMed:27281216).Gasdermin-D, N-terminalPromotes pyroptosis in response to microbial infection and danger signals (PubMed:26375003, PubMed:26375259, PubMed:27418190, PubMed:28392147, PubMed:32820063). Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1, CASP4 or CASP5 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators (PubMed:26375003, PubMed:26375259, PubMed:27418190). After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine (PubMed:27281216, PubMed:29898893). Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature IL1B and triggering pyroptosis (PubMed:27418190, PubMed:27281216, PubMed:29898893). Exhibits bactericidal activity (PubMed:27281216). Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity (PubMed:27281216). Under cell culture conditions, also active against intracellular bacteria, such as Listeria monocytogenes (By similarity). Also active in response to MAP3K7/TAK1 inactivation by Yersinia toxin YopJ, which triggers cleavage by CASP8 and subsequent activation (By similarity). Strongly binds to bacterial and mitochondrial lipids, including cardiolipin (PubMed:27281216). Does not bind to unphosphorylated phosphatidylinositol, phosphatidylethanolamine nor phosphatidylcholine (PubMed:27281216).

Alternative names

Gasdermin-D, Gasdermin domain-containing protein 1, GSDMD, DFNA5L, GSDMDC1, FKSG10

Recommended products

Rabbit Recombinant Monoclonal GSDMD antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR20885-203

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab255984 is the carrier-free version of Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] ab227821.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Cleaved C-terminal GSDMD also known as cleaved gasdermin D is a protein involved in cellular processes influencing cell death. It is a fragment derived from gasdermin D (GSDMD) after cleavage which is facilitated by inflammatory caspases. This cleavage removes an inhibitory domain allowing the active N-terminal to initiate its functions. The molecular weight of GSDMD is approximately 53 kDa. Cleaved GSDMD is often detected through methods like Western blot particularly in immune cells such as macrophages and monocytes.

Biological function summary

Cleaved C-terminal GSDMD contributes significantly to the process of pyroptosis a form of programmed cell death that is inflammation-driven. This fragmented form is important for forming pores in the cell membrane leading to cellular lysis and the release of pro-inflammatory signals. Gasdermin D including its cleaved form is not known to be part of a large complex on its own but acts autonomously after activation by other proteins like caspase-1.

Pathways

Cleaved C-terminal GSDMD participates actively in the inflammatory response particularly in the inflammasome pathway. The protein is closely linked to caspases like caspase-1 that activate it leading to pyroptotic cell death. This pathway also intersects with other innate immune components such as the NLRP3 inflammasome to magnify inflammatory signals and recruit additional immune cells to sites of infection or damage.

Associated diseases and disorders

Cleaved C-terminal GSDMD plays a role in conditions featuring excessive inflammation like septicemia and inflammatory bowel disease (IBD). In these diseases aberrant activation and overproduction of cleaved GSDMD can magnify tissue damage through uncontrolled cell death and inflammation. Also in septicemia GSDMD interacts with proteins like caspase-11 which can exacerbate inflammatory cytokine release and worsen the disease course.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] - BSA and Azide free (ab255984), expandable thumbnail

    Western blot - Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] - BSA and Azide free (ab255984)

    The image was kindly provided by our collaborator Dr Feng Shao's lab, NIBS.

    The molecular weight observed is consistent with literature (PMID: 26375003).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] ab227821).

    All lanes: Western blot - Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] (Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] ab227821) at 1/1000 dilution

    Lane 1: SiHa (human cervical squamous cell carcinoma), cell lysate plus concentrated cell supernatant at 20 µg

    Lane 2: SiHa (treated with 5 μg LPS/ 10E6 cells by electroporation for 2h.), cell lysate plus concentrated supernatant. at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/5000 dilution

    Predicted band size: 53 kDa

    Observed band size: 22 kDa

    Exposure time: 5min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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